北京华越洋生物
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首页    载体及质粒    pBAD30
PBAD30

pBAD30

pBAD30

pBAD30

 



编号

载体名称

北京华越洋VECT90122

pBAD30

 

pBAD30载体基本信息:

启动子:

araC

复制子:

p15A ori,F1 ori

质粒分类:

大肠杆菌载体;pBAD系列表达质粒

质粒大小:

4919bp

原核抗性:

氨苄青霉素Amp

克隆菌株:

Top10

培养条件:

37,有氧,LB

表达宿主:

推荐LMG194

诱导方式:

阿拉伯糖

5'测序引物:

pBAD-F:   ATGCCATAGCATTTTTATCC

3'测序引物:

pBAD-R: GATTTAATCTGTATCAGG

 

pBAD30载体质粒图谱和多克隆位点信息:

 

pBAD30载体简介

pBAD30载体序列:

ORIGIN
        1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC
       61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA
      121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA
      181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA
      241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG
      301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG
      361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG
      421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT
      481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC
      541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC
      601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA
      661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA
      721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA
      781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA
      841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC
      901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT
      961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT
     1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA
     1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT
     1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA
     1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA
     1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAATT CGAGCTCGGT
     1321 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA
     1381 GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG
     1441 AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG
     1501 AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG
     1561 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT
     1621 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA
     1681 GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA
     1741 GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT
     1801 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     1861 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     1921 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     1981 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     2041 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     2101 TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     2161 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     2221 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     2281 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     2341 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     2401 ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     2461 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     2521 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     2581 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     2641 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     2701 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     2761 CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG
     2821 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT
     2881 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG
     2941 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG
     3001 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG
     3061 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC
     3121 TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT
     3181 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA
     3241 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT
     3301 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     3361 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     3421 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     3481 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     3541 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTCAGGCATT TGAGAAGCAC
     3601 ACGGTCACAC TGCTTCCGGT AGTCAATAAA CCGGTAAACC AGCAATAGAC ATAAGCGGCT
     3661 ATTTAACGAC CCTGCCCTGA ACCGACGACC GGGTCGAATT TGCTTTCGAA TTTCTGCCAT
     3721 TCATCCGCTT ATTATCACTT ATTCAGGCGT AGCACCAGGC GTTTAAGGGC ACCAATAACT
     3781 GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG
     3841 CATTCTGCCG ACATGGAAGC CATCACAGAC GGCATGATGA ACCTGAATCG CCAGCGGCAT
     3901 CAGCACCTTG TCGCCTTGCG TATAATATTT GCCGCTAGCG GAGTGTATAC TGGCTTACTA
     3961 TGTTGGCACT GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA AAAGGCTGCA
     4021 CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT CACTGACTCG
     4081 CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT ACGAACGGGG CGGAGATTTC
     4141 CTGGAAGATG CCAGGAAGAT ACTTAACAGG GAAGTGAGAG GGCCGCGGCA AAGCCGTTTT
     4201 TCCATAGGCT CCGCCCCCCT GACAAGCATC ACGAAATCTG ACGCTCAAAT CAGTGGTGGC
     4261 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGCGGCTCC CTCGTGCGCT
     4321 CTCCTGTTCC TGCCTTTCGG TTTACCGGTG TCATTCCGCT GTTATGGCCG CGTTTGTCTC
     4381 ATTCCACGCC TGACACTCAG TTCCGGGTAG GCAGTTCGCT CCAAGCTGGA CTGTATGCAC
     4441 GAACCCCCCG TTCAGTCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     4501 CCGGAAAGAC ATGCAAAAGC ACCACTGGCA GCAGCCACTG GTAATTGATT TAGAGGAGTT
     4561 AGTCTTGAAG TCATGCGCCG GTTAAGGCTA AACTGAAAGG ACAAGTTTTG GTGACTGCGC
     4621 TCCTCCAAGC CAGTTACCTC GGTTCAAAGA GTTGGTAGCT CAGAGAACCT TCGAAAAACC
     4681 GCCCTGCAAG GCGGTTTTTT CGTTTTCAGA GCAAGAGATT ACGCGCAGAC CAAAACGATC
     4741 TCAAGAAGAT CATCTTATTA ATCAGATAAA ATATTTGCTC ATGAGCCCGA AGTGGCGAGC
     4801 CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC
     4861 GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCTGC TCATGTTTGA CAGCTTATC
//

 

pBAD30其他相关pBad系列表达载体:

pETBlue-2

pQE-30   UA   (linearized)

pET30a-EcoRV

pQE-22

pET28a-SUMO

pQE-19

pET28a-OFP

pQE-18

pET28a-mCherry

pQE-17

pET28a-EYFP

pQE-15

pET28a-EGFP

pQE-14

pET28a-ECFP

pQE-13

pET28a-EBFP

pQE-12

pET28a-DsRed2

pQE-11

pTrc-CKS

pQE-10

pMBP-P

pQE-8

pMBP-C

pQE-7

pLpp-OmpA

pQE-6

pLLP-STII/Plpp-STII

pQE-5

pET-Trx

pQE-4

pETM-30

pQE-3

pETM-11

pCold-SUMO-His-FLAG

pET-GST

pCold-SUMO-His-FLAG

pET-DsbA

pCold-SUMO

pET22b-EGFP

pCold-NusA

TAGZyme     pQE-2

pCold-MBP

TAGZyme     pQE-1

pCold-DsbA

pQE-TriSystem-7

pUC57-Kan

pQE-TriSystem-6

pUC57-Simple

pQE-TriSystem-5

pUC57

pQE-TriSystem     Strep

pUC119

pQE-TriSystem     His-Strep2

pUC118

pQE-TriSystem     His-Strep1

pJET1.2   T   Recombined

pQE-T7-2

pMD-19TSimple

pQE-T7-1

pGEM-3Zf(+)

pQE-T7(cloning     region)

pBluescript     II SK(+)

pQE-100     DoubleTaq

pBluescript     II KS(+)

pQE-82L-Kan

pUC19

pQE-81L-Kan

pUC18

pQE-80L-Kan

pMD-18T

pQE-80

pUC57-Tac-mCherry

pQE-52

pTYB21

pQE-51

pTYB4

pQE-50

pTrcHisC

pQE-42

pTrcHisB

pQE-40

pTrcHisA

pQE-30   Xa

pTrcHis2C

pQE-30   UA

pTrcHis2B

pTrcHis2A

pBAD/Myc-HisA

pRSETC

pBAD/His     /LacZ

pRSETB

pBAD/His-C

pMCSG19

pBAD/His-B

pMCSG9

pBAD/His-A

pLAFR-3

pBAD30

pEGFP-Lac

pGEX-Zsgreen1

pDrive

pGEX-TagRFP

pBV222

pGEX-His

pBAD/Myc-HisC

pGEX-20T

pBAD/Myc-HisB

pGEX-1λT     EcoRI/BAP