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TEV protease

TEV protease

TEV protease



编号

载体名称

北京华越洋VECT75331

TEV protease

 

 

TEV protease载体基本信息:

载体名称:

TEV   protease

质粒类型:

大肠杆菌表达载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

--

5'   测序引物及序列:

--

3'   测序引物及序列:

--

载体标签:

6xHis

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

 

 

TEV protease载体简介:

 

AcTEV Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it

 

useful for removing affinity tags from fusion proteins. AcTEV Protease is an improved version of Tobacco Etch Virus (TEV) protease that is

 

highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity. AcTEV

 

Protease features:

 

Highly specific cleavage activity

Enhanced enzyme stability for prolonged protease activity

Activity over a broad temperature (+4°C to 30°C) and pH (6.0 to 8.5) range

Six-histidine sequence to facilitate its removal from the digested protein sample

Greater than 85% single-band purity with no nonspecific protease contaminationApplications

Incubation with AcTEV Protease releases the protein of interest from the fusion tag. This is an effective way to remove solubility, secretion,

 

detection, and purification tags from recombinant proteins.Enzyme specifications

Purified from E. coli expressing the AcTEV Protease gene.

 

Unit definition

One unit of AcTEV Protease cleaves 85% of a 3 µg control substrate in 1 hr at 30°C.

 

Unit reaction conditions

50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 1 mM DTT, 3 µg control substrate, and 1 unit enzyme in 30 µL for 1 hr at 30°C. AcTEV Protease is

 

functionally tested for the absence of any nonspecific protease activity.

 

 

 

TEV protease载体其他相关的其他类型载体:

TOPFlash

pISRE-TA-Luc

TEV protease

pIL253

SuperTopFlash

pIJ8860

pZsGreen1-DR

pHY300PLK

pZsGreen1-1

pHY300

pZL507

pHT304

pYCT

pHT43

pXMJ19

pHT01

pVLT33

pHSE-Luc

pUG6

pHIS1525

pTimer-1

pHGF9050

pTimer

pHD_v2

pTA-Luc

pHcRed1-DR

pTALETF_v2 (NN)

pHcRed1-1

pTALETF_v2 (NI)

pHCMC05

pTALETF_v2 (NG)

pHCMC04

pTALETF_v2 (HD)

pGRE-luc

pTALEN_v2 (NN)

pGFP315

pTALEN_v2 (NI)

pGFP22

pTALEN_v2 (NG)

pGEM-3

pTALEN_v2 (HD)

pGAS-TA-Luc

pSRE-Luc

pE2F-TA-Luc

pSH65

pDsRed-Express-DR

pSH63

pDsRed-Express2-1

pSH47

pDsRed-Express-1

pSG1154

pDsRed2-1

pset152

pDG1363

pRK2013

pCSN44

pQE-TriSystem

pDD-tdTomato Reporter

pOS7001

pCRE-Luc

pNZ8048

pCR-Blunt

pNN_v2

pCMV-Raf-1

pNI v2

pCMV-p53

pNG_v2

pCMV-IκBα

pMUTIN4

pCMV-CREB

pMG36e

pCaspase3-sensor

pMetLuc2-Reporter

pCantab 5E

pMetLuc2-Control

pBT2

pmCherry-1

pBR322

pMA09-J

pBIG2R-HPH2

pMA09-H

pAP-1-Luc

pMA5MCS

pAN8-1

pKOR1

pAD43-25

pkc1139

pAD43-25

pK18mobSacB

pAcGFP1-1

pJLA502

HRV 3C protease

pIκB-EGFP

GG499

pAN7-1

p53-Luc

pAP1(PMA)-TA-Luc