pUG6

pUG6

pUG6载体质粒图谱和多克隆位点信息

pUG6载体简介：

Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30114.

Transformed E.coli cells are resistant to ampicillin and kanamycin. The plasmid contains the kanMX gene from Tn903 that confers resistance to the aminoglycosid antibiotic G418 of transformed yeasts. Due to the flanking loxP sites the deletion marker can be removed by expressing cre recombinase.

Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants.

pUG6载体序列：

ORIGIN      

        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acaaccctta atataacttc

       61 gtataatgta tgctatacga agttattagg tctagagatc tgtttagctt gcctcgtccc

      121 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg

      181 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg

      241 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct

      301 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc

      361 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc

      421 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac

      481 aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca acatggatgc

      541 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta

      601 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt

      661 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct

      721 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat

      781 ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt

      841 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt

      901 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt

      961 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga

     1021 aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact

     1081 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg

     1141 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc

     1201 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt

     1261 gcagtttcat ttgatgctcg atgagttttt ctaatcagta ctgacaataa aaagattctt

     1321 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca

     1381 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta

     1441 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg

     1501 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctctcgaga acccttaata

     1561 taacttcgta taatgtatgc tatacgaagt tattaggtga tatcagatcc actagtggcc

     1621 tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag

     1681 gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc

     1741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta

     1801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag

     1861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg

     1921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg

     1981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg

     2041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga

     2101 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc

     2161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt

     2221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact

     2281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg

     2341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt

     2401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt

     2461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct

     2521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg

     2581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt

     2641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt

     2701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

     2761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

     2821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

     2881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

     2941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca

     3001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

     3061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

     3121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

     3181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

     3241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata

     3301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

     3361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

     3421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

     3481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

     3541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga

     3601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

     3661 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg

     3721 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac

     3781 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc

     3841 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca

     3901 gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg cggctacaat

     3961 taatacataa ccttatgtat catacacata cgatttaggt gacactata

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pUG6载体其他相关的其他类型载体：