pTALETF_v2 (NI)
pTALETF_v2 (NI)
pTALETF_v2 (NI)
编号 | 载体名称 |
北京华越洋VECT75346 | pTALETF_v2 (NI) |
pTALETF_v2 (NI)载体基本信息：
载体名称: | pTALETF_v2 (NI) |
质粒类型: | 哺乳细胞表达；靶向基因操作载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 8901 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | Ampicillin 和Chloramphenicol |
筛选标记: | -- |
备注: | 克隆菌株为ccdB Survival。 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pTALETF_v2 (NI)载体质粒图谱和多克隆位点信息
pTALETF_v2 (NI)载体序列：
ORIGIN
1 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGCGCGTC GTTCCACTGA GCGTCAGACC
61 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
121 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
181 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG
241 TGTAGCCGTA GTTAGCCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC
301 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG
361 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
421 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT
481 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG
541 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
601 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC
661 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
721 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG
781 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA
841 GCGAGGAAGC GGAAGGCGAG AGTAGGGAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCCC
901 TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTCTGT GTTGTAAAAC GACGGCCAGT
961 CTTAAGCTCG GGCCCCCTGG GCGGTTCTGA TAACGAGTAA TCGTTAATCC GCAAATAACG
1021 TAAAAACCCG CTTCGGCGGG TTTTTTTATG GGGGGAGTTT AGGGAAAGAG CATTTGTCAG
1081 AATATTTAAG GGCGCCTGTC ACTTTGCTTG ATATATGAGA ATTATTTAAC CTTATAAATG
1141 AGAAAAAAGC AACGCACTTT AAATAAGATA CGTTGCTTTT TCGATTGATG AACACCTATA
1201 ATTAAACTAT TCATCTATTA TTTATGATTT TTTGTATATA CAATATTTCT AGTTTGTTAA
1261 AGAGAATTAA GAAAATAAAT CTCGAAAATA ATAAAGGGAA AATCAGTTTT TGATATCAAA
1321 ATTATACATG TCAACGATAA TACAAAATAT AATACAAACT ATAAGATGTT ATCAGTATTT
1381 ATTATCATTT AGAATAAATT TTGTGTCGCC CTTAATTGTG AGCGGATAAC AATTACGAGC
1441 TTCATGCACA GTGGCGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
1501 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
1561 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
1621 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
1681 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
1741 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
1801 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA
1861 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG
1921 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT
1981 CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
2041 CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT
2101 ATAGGGGCGT ACGGCCACCA TGTCGCGGAC CCGGCTCCCT TCCCCACCCG CACCCAGCCC
2161 AGCGTTTTCG GCCGACTCGT TCTCAGACCT GCTTAGGCAG TTCGACCCCT CACTGTTTAA
2221 CACATCGTTG TTCGACTCCC TTCCTCCGTT TGGGGCGCAC CATACGGAGG CGGCCACCGG
2281 GGAGTGGGAT GAGGTGCAGT CGGGATTGAG AGCTGCGGAT GCACCACCCC CAACCATGCG
2341 GGTGGCCGTC ACCGCTGCCC GACCGCCGAG GGCGAAGCCC GCACCAAGGC GGAGGGCAGC
2401 GCAACCGTCC GACGCAAGCC CCGCAGCGCA AGTAGATTTG AGAACTTTGG GATATTCACA
2461 GCAGCAGCAG GAAAAGATCA AGCCCAAAGT GAGGTCGACA GTCGCGCAGC ATCACGAAGC
2521 GCTGGTGGGT CATGGGTTTA CACATGCCCA CATCGTAGCC TTGTCGCAGC ACCCTGCAGC
2581 CCTTGGCACG GTCGCCGTCA AGTACCAGGA CATGATTGCG GCGTTGCCGG AAGCCACACA
2641 TGAGGCGATC GTCGGTGTGG GGAAACAGTG GAGCGGAGCC CGAGCGCTTG AGGCCCTGTT
2701 GACGGTCGCG GGAGAGCTGA GAGGGCCTCC CCTTCAGCTG GACACGGGCC AGTTGCTGAA
2761 GATCGCGAAG CGGGGAGGAG TCACGGCGGT CGAGGCGGTG CACGCGTGGC GCAATGCGCT
2821 CACGGGAGCA CCCCTCAACC TGACAgagac cGCGGCCGCA TTAGGCACCC CAGGCTTTAC
2881 ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC
2941 AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC
3001 CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA
3061 CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA
3121 GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG
3181 TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT
3241 TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG
3301 GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT
3361 CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC
3421 CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG
3481 CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC
3541 CGTTTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA
3601 GTGGCAGGGC GGGGCGTAAA GATCTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC
3661 GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT
3721 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
3781 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA
3841 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
3901 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GGCTGGTGAA
3961 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
4021 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
4081 CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
4141 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
4201 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
4261 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACggtc tcGACTCACG
4321 CCTGAGCAGG TAGTGGCTAT TGCATCCAAT ATCGGGGGCA GACCCGCACT GGAGTCAATC
4381 GTGGCCCAGC TTTCGAGGCC GGACCCCGCG CTGGCCGCAC TCACTAATGA TCATCTTGTA
4441 GCGCTGGCCT GCCTCGGCGG ACGACCCGCC TTGGATGCGG TGAAGAAGGG GCTCCCGCAC
4501 GCGCCTGCAT TGATTAAGCG GACCAACAGA AGGATTCCCG AGAGGACATC ACATCGAGTG
4561 GCAGATCACG CGCAAGTGGT CCGCGTGCTC GGATTCTTCC AGTGTCACTC CCACCCCGCA
4621 CAAGCGTTCG ATGACGCCAT GACTCAATTT GGTATGTCGA GACACGGACT GCTGCAGCTC
4681 TTTCGTAGAG TCGGTGTCAC AGAACTCGAG GCCCGCTCGG GCACACTGCC TCCCGCCTCC
4741 CAGCGGTGGG ACAGGATTCT CCAAGCGAGC GGTATGAAAC GCGCGAAGCC TTCACCTACG
4801 TCAACTCAGA CACCTGACCA GGCGAGCCTT CATGCGTTCG CAGACTCGCT GGAGAGGGAT
4861 TTGGACGCGC CCTCGCCCAT GCATGAAGGG GACCAAACTC GCGCGTCAGC TAGCCCCAAG
4921 AAGAAGAGAA AGGTGGAGGC CAGCGGTTCC GGACGGGCTG ACGCATTGGA CGATTTTGAT
4981 CTGGATATGC TGGGAAGTGA CGCCCTCGAT GATTTTGACC TTGACATGCT TGGTTCGGAT
5041 GCCCTTGATG ACTTTGACCT CGACATGCTC GGCAGTGACG CCCTTGATGA TTTCGACCTG
5101 GACATGCTGA TTAACTCTAG AGGCAGTGGA GAGGGCAGAG GAAGTCTGCT AACATGCGGT
5161 GACGTCGAGG AGAATCCTGG CCCAGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
5221 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
5281 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG
5341 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC
5401 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
5461 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
5521 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
5581 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
5641 ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG
5701 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
5761 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC
5821 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
5881 ATGGACGAGC TGTACAAGTA AGAATTCAAA ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
5941 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
6001 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCACAACAC TCAACCCTAT CTCGGTCTAT
6061 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
6121 TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT
6181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
6241 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT
6301 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT
6361 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG
6421 CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
6481 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGATGAAAA
6541 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT
6601 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG
6661 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG
6721 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT
6781 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC
6841 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG
6901 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC
6961 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC
7021 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC
7081 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA
7141 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG
7201 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG
7261 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC
7321 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT
7381 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA
7441 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG
7501 AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGAGGGCA AAGGAATAGC
7561 ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG
7621 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG
7681 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
7741 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
7801 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT
7861 CATTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
7921 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
7981 CCCAGCGCTG CGATGATACC GCGAGAACCA CGCTCACCGG CTCCGGATTT ATCAGCAATA
8041 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
8101 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
8161 AACGTTGTTG CCATCGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
8221 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
8281 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
8341 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
8401 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
8461 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
8521 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
8581 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
8641 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
8701 ACACGGAAAT GTTGAATACT CATATTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
8761 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
8821 GTCAGTGTTA CAACCAATTA ACCAATTCTG AACATTATCG CGAGCCCATT TATACCTGAA
8881 TATGGCTCAT AACACCCCTT G
//
pTALETF_v2 (NI)载体其他相关的其他类型载体：
