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首页    载体及质粒    pIκB-EGFP
pIκB-EGFP载体图谱

pIκB-EGFP

pIκB-EGFP

pIκB-EGFP

 



编号

载体名称

北京华越洋VECT75383

PIkB-EGFP

 

PIkB-EGFP载体基本信息:

载体名称:

PIkB-EGFP

质粒类型:

信号通路报告系统载体

高拷贝/低拷贝:

--

启动子:

CMV

克隆方法:

多克隆位点,限制性内切酶

载体大小:

5.7kb

5'   测序引物及序列:

--

3'   测序引物及序列:

--

载体标签:

EGFP

载体抗性:

卡纳霉素(Kanamycin)

筛选标记:

Neomycin_1.html'   target='_blank'>新霉素(Neomycin)

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

PIkB-EGFP载体质粒图谱和多克隆位点信息:

 

PIkB-EGFP载体简介:

 

The pIκB-EGFP Vector encodes the IκB-EGFP Signaling Probe, which is a fusion of enhanced green

fluorescent protein (EGFP) and IκB. IκB is an inhibitor of NFκB, a transcription factor involved in the

immune response and inflammatory diseases. When the NFκB pathway is inactive, IκB and NFκB

exist as an inactive complex in the cytosol. Upon stimulation, IκB is degraded. In cells transfected

with pIκB-EGFP, degradation of the IκB-EGFP fusion protein is observed as a decrease in EGFP

fluorescence (1). The IκB-EGFP Signaling Probe is constitutively expressed and resides in the

cytosol.

EGFP is a red-shifted, human codon-optimized variant of GFP (26) that has been engineered for

brighter fluorescence and higher expression in mammalian cells. Its excitation maximum is 488 nm

and emission maximum is 509 nm. For more information on the properties of EGFP, please refer to

the BD Living Colors? User Manual (PT2040-1) included with the vector.

Expression of the IκB-EGFP is driven by the human CMV immediate-early promoter. The SV40 poly-A

sequence directs proper processing of the 3' end of the fusion construct. The vector backbone also

contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A

neomycin resistance cassette (Neor) allows kanamycin selection in E. coli and neomycin (G418)

selection in eukaryotic cells. This cassette consists of the SV40 early promoter, the neomycin/

kanamycin resistance gene of Tn5, and poly-A signals from the Herpes simplex virus thymidine

kinase (HSV TK) gene. The vector backbone also provides a pUC origin of replication for

propagation in E. coli and an f1 origin for single-stranded DNA production.

 

 

PIkB-EGFP载体其他相关的其他类型载体:

TOPFlash

pISRE-TA-Luc

TEV protease

pIL253

SuperTopFlash

pIJ8860

pZsGreen1-DR

pHY300PLK

pZsGreen1-1

pHY300

pZL507

pHT304

pYCT

pHT43

pXMJ19

pHT01

pVLT33

pHSE-Luc

pUG6

pHIS1525

pTimer-1

pHGF9050

pTimer

pHD_v2

pTA-Luc

pHcRed1-DR

pTALETF_v2 (NN)

pHcRed1-1

pTALETF_v2 (NI)

pHCMC05

pTALETF_v2 (NG)

pHCMC04

pTALETF_v2 (HD)

pGRE-luc

pTALEN_v2 (NN)

pGFP315

pTALEN_v2 (NI)

pGFP22

pTALEN_v2 (NG)

pGEM-3

pTALEN_v2 (HD)

pGAS-TA-Luc

pSRE-Luc

pE2F-TA-Luc

pSH65

pDsRed-Express-DR

pSH63

pDsRed-Express2-1

pSH47

pDsRed-Express-1

pSG1154

pDsRed2-1

pset152

pDG1363

pRK2013

pCSN44

pQE-TriSystem

pDD-tdTomato Reporter

pOS7001

pCRE-Luc

pNZ8048

pCR-Blunt

pNN_v2

pCMV-Raf-1

pNI v2

pCMV-p53

pNG_v2

pCMV-IκBα

pMUTIN4

pCMV-CREB

pMG36e

pCaspase3-sensor

pMetLuc2-Reporter

pCantab 5E

pMetLuc2-Control

pBT2

pmCherry-1

pBR322

pMA09-J

pBIG2R-HPH2

pMA09-H

pAP-1-Luc

pMA5MCS

pAN8-1

pKOR1

pAD43-25

pkc1139

pAD43-25

pK18mobSacB

pAcGFP1-1

pJLA502

HRV 3C protease

pIκB-EGFP

GG499

pAN7-1

p53-Luc

pAP1(PMA)-TA-Luc