pHcRed1-1
pHcRed1-1
pHcRed1-1
编号 | 载体名称 |
北京华越洋VECT75328 | pHcRed1-1 |
pHcRed1-1载体基本信息：
载体名称: | pHcRed1-1 |
质粒类型: | 无启动子载体；荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | 无启动子 |
载体大小: | 4118 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | 红色荧光蛋白HcRed1 |
载体抗性: | 卡那霉素 |
筛选标记: | Neomycin_1.html' target='_blank'>新霉素（Neomycin） |
克隆菌株: | TOP10, DH5α, HB101 |
宿主细胞（系）: | 真核细胞 |
备注: | pHcRed1-1载体不含启动子，将待研究的目的启动子插入MCS区，用于研究启动子及顺式转录元件在细胞内的作用水平。 |
稳定性: | 瞬表达 或 稳表达 |
组成型/诱导型: | -- |
病毒/非病毒: | 非病毒 |
pHcRed1-1载体质粒图谱和多克隆位点信息：
pHcRed1-1载体序列：
ORIGIN
1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCGAAT TCTGCAGTCG
61 ACGGTACCGC GGGCCCGGGA TCCACCGGTC GCCACCATGG TGAGCGGCCT GCTGAAGGAG
121 AGTATGCGCA TCAAGATGTA CATGGAGGGC ACCGTGAACG GCCACTACTT CAAGTGCGAG
181 GGCGAGGGCG ACGGCAACCC CTTCGCCGGC ACCCAGAGCA TGAGAATCCA CGTGACCGAG
241 GGCGCCCCCC TGCCCTTCGC CTTCGACATC CTGGCCCCCT GCTGCGAGTA CGGCAGCAGG
301 ACCTTCGTGC ACCACACCGC CGAGATCCCC GACTTCTTCA AGCAGAGCTT CCCCGAGGGC
361 TTCACCTGGG AGAGAACCAC CACCTACGAG GACGGCGGCA TCCTGACCGC CCACCAGGAC
421 ACCAGCCTGG AGGGCAACTG CCTGATCTAC AAGGTGAAGG TGCACGGCAC CAACTTCCCC
481 GCCGACGGCC CCGTGATGAA GAACAAGAGC GGCGGCTGGG AGCCCAGCAC CGAGGTGGTG
541 TACCCCGAGA ACGGCGTGCT GTGCGGCCGG AACGTGATGG CCCTGAAGGT GGGCGACCGG
601 CACCTGATCT GCCACCACTA CACCAGCTAC CGGAGCAAGA AGGCCGTGCG CGCCCTGACC
661 ATGCCCGGCT TCCACTTCAC CGACATCCGG CTCCAGATGC TGCGGAAGAA GAAGGACGAG
721 TACTTCGAGC TGTACGAGGC CAGCGTGGCC CGGTACAGCG ACCTGCCCGA GAAGGCCAAC
781 TGAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG
841 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
901 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
961 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
1021 TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT
1081 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
1141 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG
1201 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT
1261 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC
1321 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG
1381 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
1441 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT
1501 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
1561 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
1621 CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG
1681 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG
1741 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
1801 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC
1861 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG
1921 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG
1981 ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
2041 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
2101 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
2161 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG
2221 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
2281 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
2341 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
2401 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
2461 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
2521 CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
2581 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT
2641 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
2701 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC
2761 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG
2821 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG
2881 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
2941 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA
3001 CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA
3061 AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT
3121 CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC
3181 CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG
3241 GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
3301 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
3361 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
3421 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
3481 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
3541 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
3601 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
3661 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
3721 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
3781 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
3841 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
3901 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
3961 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
4021 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
4081 CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT
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pHcRed1-1载体其他相关的其他类型载体：
