北京华越洋生物
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首页    载体及质粒    pENTR-GUS
pENTR-GUSMap

pENTR-GUS

pENTR-GUS

pENTR-GUS

 



编号

载体名称

北京华越洋VECT511131

pENTR-GUS

 

pENTR-GUS  Gateway载体基本信息:

载体名称:

pENTR-GUS

质粒类型:

Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

3841 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

克隆菌株:

DH5aTOP10感受态细胞

备注:

--

病毒/非病毒:

非病毒

 

pENTR-GUS载体质粒图谱和多克隆位点信息:

pENTR-GUS载体简介

pENTR-GUS载体序列:

ORIGIN

        1 TTTTATTGTT CATGATGATA TATTTTTATC TTGTGCAATG TAACATCAGA GATTTTGAGA

       61 CACGGGCCAG AGCTGCAGCT AGCATGGATC TCGGGCCCCA AATAATGATT TTATTTTGAC

      121 TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT

      181 TGTACAAAAA AGCAGGCTCA TTTAACTTTA AGAAGGAGAT ATATACCATG GTCCGTCCTG

      241 TAGAAACCCC AACCCGTGAA ATCAAAAAAC TCGACGGCCT GTGGGCATTC AGTCTGGATC

      301 GCGAAAACTG TGGAATTGAT CAGCGTTGGT GGGAAAGCGC GTTACAAGAA AGCCGGGCAA

      361 TTGCTGTGCC AGGCAGTTTT AACGATCAGT TCGCCGATGC AGATATTCGT AATTATGCGG

      421 GCAACGTCTG GTATCAGCGC GAAGTCTTTA TACCGAAAGG TTGGGCAGGC CAGCGTATCG

      481 TGCTGCGTTT CGATGCGGTC ACTCATTACG GCAAAGTGTG GGTCAATAAT CAGGAAGTGA

      541 TGGAGCATCA GGGCGGCTAT ACGCCATTTG AAGCCGATGT CACGCCGTAT GTTATTGCCG

      601 GGAAAAGTGT ACGTATCACC GTTTGTGTGA ACAACGAACT GAACTGGCAG ACTATCCCGC

      661 CGGGAATGGT GATTACCGAC GAAAACGGCA AGAAAAAGCA GTCTTACTTC CATGATTTCT

      721 TTAACTATGC CGGAATCCAT CGCAGCGTAA TGCTCTACAC CACGCCGAAC ACCTGGGTGG

      781 ACGATATCAC CGTGGTGACG CATGTCGCGC AAGACTGTAA CCACGCGTCT GTTGACTGGC

      841 AGGTGGTGGC CAATGGTGAT GTCAGCGTTG AACTGCGTGA TGCGGATCAA CAGGTGGTTG

      901 CAACTGGACA AGGCACTAGC GGGACTTTGC AAGTGGTGAA TCCGCACCTC TGGCAACCGG

      961 GTGAAGGTTA TCTCTATGAA CTGTGCGTCA CAGCCAAAAG CCAGACAGAG TGTGATATCT

     1021 ACCCGCTTCG CGTCGGCATC CGGTCAGTGG CAGTGAAGGG CGAACAGTTC CTGATTAACC

     1081 ACAAACCGTT CTACTTTACT GGCTTTGGTC GTCATGAAGA TGCGGACTTA CGTGGCAAAG

     1141 GATTCGATAA CGTGCTGATG GTGCACGACC ACGCATTAAT GGACTGGATT GGGGCCAACT

     1201 CCTACCGTAC CTCGCATTAC CCTTACGCTG AAGAGATGCT CGACTGGGCA GATGAACATG

     1261 GCATCGTGGT GATTGATGAA ACTGCTGCTG TCGGCTTTAA CCTCTCTTTA GGCATTGGTT

     1321 TCGAAGCGGG CAACAAGCCG AAAGAACTGT ACAGCGAAGA GGCAGTCAAC GGGGAAACTC

     1381 AGCAAGCGCA CTTACAGGCG ATTAAAGAGC TGATAGCGCG TGACAAAAAC CACCCAAGCG

     1441 TGGTGATGTG GAGTATTGCC AACGAACCGG ATACCCGTCC GCAAGGTGCA CGGGAATATT

     1501 TCGCGCCACT GGCGGAAGCA ACGCGTAAAC TCGACCCGAC GCGTCCGATC ACCTGCGTCA

     1561 ATGTAATGTT CTGCGACGCT CACACCGATA CCATCAGCGA TCTCTTTGAT GTGCTGTGCC

     1621 TGAACCGTTA TTACGGATGG TATGTCCAAA GCGGCGATTT GGAAACGGCA GAGAAGGTAC

     1681 TGGAAAAAGA ACTTCTGGCC TGGCAGGAGA AACTGCATCA GCCGATTATC ATCACCGAAT

     1741 ACGGCGTGGA TACGTTAGCC GGGCTGCACT CAATGTACAC CGACATGTGG AGTGAAGAGT

     1801 ATCAGTGTGC ATGGCTGGAT ATGTATCACC GCGTCTTTGA TCGCGTCAGC GCCGTCGTCG

     1861 GTGAACAGGT ATGGAATTTC GCCGATTTTG CGACCTCGCA AGGCATATTG CGCGTTGGCG

     1921 GTAACAAGAA AGGGATCTTC ACTCGCGACC GCAAACCGAA GTCGGCGGCT TTTCTGCTGC

     1981 AAAAACGCTG GACTGGCATG AACTTCGGTG AAAAACCGCA GCAGGGAGGC AAACAATAAT

     2041 ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA

     2101 ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT AGCGGTAATA

     2161 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA

     2221 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT

     2281 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA

     2341 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG

     2401 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA

     2461 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA

     2521 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG

     2581 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG

     2641 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA

     2701 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC

     2761 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG

     2821 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC

     2881 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGCTTGCG CCGTCCCGTC

     2941 AAGTCAGCGT AATGCTCTGC CAGTGTTACA ACCAATTAAC CAATTCTGAT TAGAAAAACT

     3001 CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT

     3061 GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA

     3121 GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC

     3181 CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG

     3241 AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT

     3301 CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA

     3361 GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC

     3421 GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA

     3481 CCTGGAATGC TGTTTTTCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC

     3541 GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA

     3601 TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG

     3661 CATCGGGCTT CCCATACAAG CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG

     3721 CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTCGAGCAAG

     3781 ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA

     3841 G

//

 

pENTR-GUS其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39