PX852
PX852
编号 | 载体名称 |
北京华越洋VECT75261 | PX852 |
PX852载体基本信息：
载体名称: | PX852 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | Age1，EcoR1 |
启动子: | CBh |
载体大小: | 7201 bp |
5' 测序引物及序列: | ctggagcacctgcctgaaatcact |
3' 测序引物及序列: | GGCTGATCAGCGAGCTCTAG |
载体标签: | NLS、SV40、FKBP12(N ter)；NLS nucleoplasmin(C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | A portion of this plasmid was derived from a plasmid made by SpCas9 piece was PCR amplified of PX330 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
PX852载体质粒图谱和多克隆位点信息：
PX852载体序列：
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGGCCCCAA
1261 AGAAGAAGCG GAAGGTCGGT ATCCACGGAG TCCCAGCAGC CGGCGTGCAG GTGGAGACCA
1321 TCAGCCCCGG CGACGGCAGA ACCTTCCCCA AGAGAGGCCA GACCTGCGTG GTGCACTACA
1381 CCGGCATGCT GGAGGACGGC AAGAAGTTCG ACAGCAGCAG AGACAGAAAC AAGCCCTTCA
1441 AGTTCATGCT GGGCAAGCAG GAGGTGATCA GAGGCTGGGA GGAGGGCGTG GCCCAGATGA
1501 GCGTGGGCCA GAGAGCCAAG CTGACCATCA GCCCCGACTA CGCCTACGGC GCCACCGGCC
1561 ACCCCGGCAT CATCCCTCCT CACGCCACCC TGGTGTTCGA CGTGGAGCTG CTGAAGCTGG
1621 AGGGTGGCGG TGGCtcgGGT GGCGGTGGCt cgGGTGGCGG TGGCtcgAAG CCCGCCTTCC
1681 TGAGCGGCGA GCAGAAAAAG GCCATCGTGG ACCTGCTGTT CAAGACCAAC CGGAAAGTGA
1741 CCGTGAAGCA GCTGAAAGAG GACTACTTCA AGAAAATCGA GTGCTTCGAC TCCGTGGAAA
1801 TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCAC ATACCACGAT CTGCTGAAAA
1861 TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG GAAGATATCG
1921 TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG AAAACCTATG
1981 CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC ACCGGCTGGG
2041 GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC AAGACAATCC
2101 TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG ATCCACGACG
2161 ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG GGCGATAGCC
2221 TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA
2281 CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC GAGAACATCG
2341 TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA
2401 GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG AAAGAACACC
2461 CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG CAGAATGGGC
2521 GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC GATGTGGACC
2581 ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG CTGACCAGAA
2641 GCGACAAGAA CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG AAGAAGATGA
2701 AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG TTCGACAATC
2761 TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC ATCAAGAGAC
2821 AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC TCCCGGATGA
2881 ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC ACCCTGAAGT
2941 CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC GAGATCAACA
3001 ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC CTGATCAAAA
3061 AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC GACGTGCGGA
3121 AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC TTCTTCTACA
3181 GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG ATCCGGAAGC
3241 GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG GGCCGGGATT
3301 TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA AAGACCGAGG
3361 TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC GATAAGCTGA
3421 TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC CCCACCGTGG
3481 CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG
3541 TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG AATCCCATCG
3601 ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC AAGCTGCCTA
3661 AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC
3721 TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG TACCTGGCCA
3781 GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG CTGTTTGTGG
3841 AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG
3901 TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG CACCGGGATA
3961 AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC AATCTGGGAG
4021 CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC ACCAGCACCA
4081 AAGAGGTGCT GGACGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC GAGACACGGA
4141 TCGACCTGTC TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG GCCGGCCAGG
4201 CAAAAAAGAA AAAGtaagaa ttcCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG
4261 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC
4321 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA
4381 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGAgAATAG
4441 CAGGCATGCT GGGGAgcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
4501 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
4561 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
4621 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4861 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4981 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
5041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
5101 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
5161 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
5221 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
5281 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
5341 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
5401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
5521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
5641 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
6001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
6061 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
6121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
6181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
6241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
6301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
6361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6541 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6601 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6661 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
6721 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6781 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6841 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6901 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6961 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
7021 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
7081 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
7141 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
7201 t
//
PX852载体其他相关CRISPR/Cas9载体：
