pX851
pX851
编号 | 载体名称 |
北京华越洋VECT75266 | pX851 |
pX851载体基本信息：
载体名称: | pX851 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | Age1，EcoR1 |
启动子: | CBh |
载体大小: | 6181 bp |
5' 测序引物及序列: | ctggagcacctgcctgaaatcact |
3' 测序引物及序列: | GGCTGATCAGCGAGCTCTAG |
载体标签: | NES、PTK2(N ter)；FRB(C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4) |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pX851载体质粒图谱和多克隆位点信息：
pX851载体序列：
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGCTAGATT
1261 TAGCTAGCTT AATCCTCGAC AAGAAGTACA GCATCGGCCT GGACATCGGC ACCAACTCTG
1321 TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG
1381 GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG
1441 GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA
1501 AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA
1561 GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC
1621 ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT
1681 ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC
1741 TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC
1801 CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT
1861 TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC
1921 TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG
1981 GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT
2041 TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG
2101 ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC
2161 TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC
2221 CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA
2281 AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA
2341 AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA
2401 TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG
2461 AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC
2521 TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA
2581 ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG
2641 CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT
2701 GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA
2761 CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG
2821 AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAG
2881 GTGGCGGTGG CtcgGGTGGC GGTGGCtcgG GTGGCGGTGG CtcgGAGATG TGGCACGAGG
2941 GCCTGGAGGA GGCCAGCAGA CTGTACTTCG GCGAGAGAAA CGTGAAGGGC ATGTTCGAGG
3001 TGCTGGAGCC CCTGCACGCC ATGATGGAGA GAGGCCCCCA GACCCTGAAG GAGACCAGCT
3061 TCAACCAGGC CTACGGCAGA GACCTGATGG AGGCCCAGGA GTGGTGCAGA AAGTACATGA
3121 AGAGCGGCAA CGTGAAGGAC CTGACCCAGG CCTGGGACCT GTACTACCAC GTGTTCAGAA
3181 GAATCAGCAA GCAGtaagaa ttcCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG
3241 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC
3301 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA
3361 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGAgAATAG
3421 CAGGCATGCT GGGGAgcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
3481 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
3541 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
3601 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
3661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
3721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
3781 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
3841 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
3901 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
3961 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
4021 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4081 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4141 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4201 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4261 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4321 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
4381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
4441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
4501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
4561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
4621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
4681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
4741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
4801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
4861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
4921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
4981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
5221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
5341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
5401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
5641 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
5701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
5761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
5821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
5881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
5941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6181 t
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pX851载体其他相关CRISPR/Cas9载体：
