PX396
PX396
编号 | 载体名称 |
北京华越洋VECT75227 | PX396 |
PX396载体基本信息：
载体名称: | PX396 或 PX396 Parvibaculum lavamentivorans Cas9 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 10307 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter)；HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 昆虫细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
PX396载体质粒图谱和多克隆位点信息：
PX396载体序列：
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatggag aggattttcg gctttgacat cggcacaaca
1921 agtatcggat tcagcgtgat tgattacagt agcacccagt ccgcaggcaa catccagagg
1981 ctgggcgtgc gcattttccc tgaggcaagg gacccagatg ggacccccct gaaccagcag
2041 cggagacaga aacgcatgat gaggcgccag ctgcgacgga gaaggattcg ccgaaaggca
2101 ctgaatgaga cactgcacga agccggcttt ctgccagctt acgggtctgc agattggccc
2161 gtggtcatgg ccgacgagcc ttatgaactg cggagaaggg gactggagga aggcctgagt
2221 gcttacgagt tcggacgggc aatctatcat ctggcccagc accggcattt taaaggcaga
2281 gaactggagg aatccgatac acccgaccct gatgtggacg atgagaagga agccgctaac
2341 gagagagcag ccactctgaa ggccctgaaa aatgaacaga ccacactggg agcatggctg
2401 gcccgccgac ccccttctga ccgcaagcga ggaatccacg cccataggaa cgtggtcgct
2461 gaggagttcg agcgcctgtg ggaagtgcag tccaagtttc accccgctct gaaatctgag
2521 gaaatgcggg caagaatcag tgatacaatt ttcgcccaga ggcctgtgtt ttggcgcaag
2581 aacactctgg gagagtgcag attcatgcct ggcgaaccac tgtgtcccaa ggggtcctgg
2641 ctgtctcagc agcggagaat gctggagaaa ctgaacaatc tggctatcgc aggcgggaat
2701 gctaggccac tggatgcaga ggaacgcgac gccattctga gtaagctgca gcagcaggcc
2761 agcatgtcct ggccaggcgt gcggtcagct ctgaaggcac tgtacaaaca gagaggcgag
2821 cccggggctg aaaagagcct gaaattcaac ctggagctgg gaggcgaatc caagctgctg
2881 ggaaatgccc tggaggctaa actggcagat atgtttggcc ctgactggcc agctcacccc
2941 cgaaagcagg agatccggca cgcagtgcat gaacggctgt gggctgcaga ttacggcgag
3001 acacccgaca agaaaagagt catcattctg tccgagaagg atcgaaaagc tcatcgggaa
3061 gccgctgcaa actctttcgt ggcagacttt ggaattactg gcgagcaggc agctcagctg
3121 caggccctga agctgccaac cggctgggaa ccttatagca tcccagcact gaacctgttc
3181 ctggccgagc tggaaaaggg ggagaggttt ggagccctgg tgaatggacc tgattgggaa
3241 ggctggaggc gcacaaactt cccccaccgc aatcagccta ctggggagat cctggacaag
3301 ctgccaagtc ccgcctcaaa agaggaaagg gaacgcatta gccagctgcg caacccaacc
3361 gtggtccgaa cacagaatga gctgagaaag gtggtcaaca atctgatcgg gctgtatgga
3421 aaacccgatc gaatccggat tgaagtgggc cgggacgtcg ggaagtccaa aagagaaagg
3481 gaggaaatcc agtctggcat tcgacggaac gagaagcaga gaaagaaagc cactgaagat
3541 ctgatcaaaa acggaattgc taatcctagc cgggacgatg tggagaagtg gatcctgtgg
3601 aaagagggcc aggaaagatg cccatacacc ggcgaccaga ttggcttcaa tgccctgttt
3661 agagaaggca gatatgaggt ggaacacatc tggcctcgct ctcgaagttt tgataacagc
3721 ccaaggaata agacactgtg tcgcaaagac gtgaacatcg agaagggaaa taggatgcct
3781 ttcgaggcat ttggccatga cgaagatcgg tggagcgcca tccagattag actgcagggc
3841 atggtgtcag ccaaaggggg aactgggatg agccccggaa aggtcaaacg cttcctggct
3901 aagaccatgc ctgaggattt tgcagcccgg cagctgaacg acacaagata cgctgcaaag
3961 cagatcctgg cccagctgaa aaggctgtgg ccagacatgg gacctgaggc tccagtgaag
4021 gtcgaagcag tgactggaca ggtcaccgcc cagctgcgca aactgtggac tctgaacaat
4081 attctggctg acgatgggga gaaaaccaga gcagatcaca ggcaccatgc catcgacgct
4141 ctgacagtgg cctgcactca tcctggaatg accaacaagc tgagcaggta ttggcagctg
4201 cgcgacgatc cacgagcaga gaagccagct ctgactccac cctgggatac catccgcgcc
4261 gacgctgaga aagccgtgtc tgaaattgtg gtcagtcacc gggtgagaaa gaaagtcagc
4321 ggcccactgc ataaggagac tacctacggc gatacaggga ctgacattaa gaccaaatcc
4381 ggcacatata gacagttcgt gaccaggaag aaaatcgagt cactgagcaa gggggagctg
4441 gatgaaattc gcgacccccg aatcaaagaa attgtggcag ctcacgtcgc aggacgagga
4501 ggcgacccca agaaggcctt ccctccatac ccctgtgtgt ctcccggagg ccctgagatc
4561 cggaaggtca gactgaccag taaacagcag ctgaacctga tggcccagac agggaatgga
4621 tacgctgacc tgggctccaa ccaccatatc gcaatctacc ggctgcccga tgggaaggcc
4681 gacttcgaga ttgtgtcact gtttgatgct agcagaaggc tggcacagag aaatccaatc
4741 gtgcagagga cacgagcaga cggagccagc ttcgtcatgt ccctggcagc cggagaggcc
4801 atcatgattc ccgaaggctc aaagaaaggg atctggattg tgcagggagt ctgggcaagc
4861 ggacaggtgg tcctggagag ggacaccgat gctgaccact ctacaactac ccgccctatg
4921 ccaaacccca tcctgaagga cgatgccaag aaagtgagta tcgatcctat tggccgagtc
4981 cggccatcaa atgactaaga attcggtacc gagctcaagc ttgcggccgc ctatagtgtc
5041 acctaaatcg taacccagct ttcttgtaca aagtggtcgg gtggcatccc tgtgacccct
5101 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
5161 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
5221 ggggggtggt atggagcaag gggcaagttg ggatgacaac ctgtagggcc tgcggggtct
5281 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
5341 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
5401 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
5461 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
5521 tacaggcgtg aaccactgct cccttccctg tccttctagc ataacccctt ggggcctcta
5581 aacgggtctt gaggggtttt ttgtctagaa gacaatagca ggcatgctgg ggatgcggtg
5641 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
5701 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
5761 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
5821 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
5881 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
5941 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
6001 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
6061 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
6121 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
6181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
6241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
6301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
6361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
6421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
6481 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
6541 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
6601 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
6661 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
6721 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
6781 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
6841 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
6901 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
6961 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
7021 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
7081 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
7141 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
7201 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
7261 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
7321 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
7381 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
7441 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
7501 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
7621 ttatcatgtc tgttcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
7681 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
7741 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
7801 catatgtaac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
7861 aaagcatgcg gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc
7921 accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta
7981 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt
8041 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt
8101 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt
8161 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt
8221 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt
8281 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt
8341 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc
8401 ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca
8461 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt
8521 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattgcgat
8581 cgcataactt cgtatagcat acattatacg aagttataag ctcggaacgc tgcgctcggt
8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
8821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
8881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
8941 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
9001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
9061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
9121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
9181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
9241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
9301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
9361 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
10261 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtt
//
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