PX386
PX386
编号 | 载体名称 |
北京华越洋VECT75237 | PX386 |
PX386载体基本信息：
载体名称: | PX386 或 PX386 Lactobacillus farciminis Cas9 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 10574 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter)；HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 昆虫细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
PX386载体质粒图谱和多克隆位点信息：
PX386载体序列：
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatggcc gaccgaatct ctctggggct ggacattggg
1921 gtggcaagcg tggggttctc agtgctggac attgataagg gaaaagtcat tgagctgggc
1981 gccaggctgt tctctgctac tgtggccgct ggcaaccagg atcgaagaga catgcgagga
2041 gccaggcgcc tgctgaaccg gaacaagcag cgacggcagg ataccggaaa gctgttcaag
2101 aaatttggcc tgatcgacga ttttgataag ggcagcttct acgacaactt taatcagaac
2161 ctgaatcctt atgagctgag agtgaaaggc ctgacagaac agctgactaa ggaggaactg
2221 gccgagtctc tgtaccagat cgtgaaacat agggggatta gttatgcact gaaggacgcc
2281 gatgtggacg aaggcgggac agactactca gtcagcctga aaatcaacag ccaggagctg
2341 gcagaaaaga ctccagccca gattcagctg cagagactga atgattatgg aaaggtgagg
2401 ggccaggtgg tcatcggcga cgatccagac aaccagaagg tgctgctgaa tgtgttcccc
2461 acatcagctt acgagaaaga agcaaagcag atcattgcca ctcagcagca gttctatcct
2521 gagagcctga ccgacaagtt caccgaggaa tactgccaga tcctgactcg caagcgagat
2581 tattttgtgg ggccaggaaa cgagaaaagc cggaccgact acgggatcta caagactgat
2641 ggaagaaccc tggacaatct gttcgaggaa ctgatcggcc acgataagat ctaccccgag
2701 gaactgcggg catctgcagc cagttatacc gcccagctgt ttaacgtgct gaatgacctg
2761 aacaatctga gaatcctgaa ctacgaggat gggaaactga caaaggagga caaggaaaag
2821 atcatcgctg aaattaagaa caacaccaca actatcaaca tgctgaatgt gattaagaaa
2881 gtcgccgggt gttccaagga cgatatcaaa ggattccgag tgaatgagaa ggataaaccc
2941 gaaatcagct ccatgcctgt gtaccgcaaa attcataagg acctgctgaa ggccggcgtg
3001 gatatctcag actggcccgt cgagttcatc gacgaactga gctttattct gacactgaac
3061 actgagaatg gggaaattcg caaacagctg aacaatcgac tggcccctaa gttcgatttt
3121 ctgaacgctg acctgatcca gctgatcatt gataataagg actcctttga gattaagact
3181 aacaacaagt ggcacagatt cagcgtgaaa accatgaaca aactgatccc agagatgatg
3241 gaaagacccg tggagcagat gaccctgctg aatgaaatgg gactggtcaa gaaagataag
3301 aaacgctttg agaacaataa gtacctgcct tacaaggaaa tcgcaaagga cattttcaac
3361 ccagtggcct ccaaatctgt ccgcgaggcc ctgaagatcg tgaatgctgt cctgaagaaa
3421 tacggccaca ttgattatct ggtggtcgaa atgcctcggg ataaaaacct gaaggaggaa
3481 caggacaata tcaaggagtt ccagaacaaa aataagaaag ctaaggacgc tgcattcgaa
3541 gcatttgtga aatcagtcgg gagcgagcag agagtgaagg aagccctgtc taaaaaccgg
3601 aagctgcaga tgaagatgag actgtggtat cagcagcagg agatcgatcc atataatgga
3661 aagacaatcg atgccactga cctgattaac aatcctgata agttcgagat tgaccatatc
3721 attccacaga gtatctcatt ttacgacagt attaacaata agaccctgtg cttcgcctca
3781 atgaaccagg tgaaaggaca gaaaaccccc tacgagttta tgctggaagg ccacgggcag
3841 tcctatgaca agttcaaagc tacagtgatg gcaaacaaga attttggcaa ggctaaaagg
3901 gcaaactacc tgttcgagga aaatgtgagc gatatcgaga ctcggaagag attcctgtcc
3961 cgcaacctgg tggacacccg atattctagt cgggtggtgc tgaacagcct gcaggatttc
4021 tttcgggaga aatctgccga caccaaggtg acagtcattc gcggcaagtt tacctccaac
4081 atgcgaaaac attggcacat cgataagact agggagacat tccaccatca cgccattgac
4141 gcttctatca ttgccgctac accatttctg cgcatgtgga agaaaggagg cactatcttc
4201 cccgtgaagg tcggagaaga aagtatcgat attgagacag gcgaaattct ggacgataag
4261 aattttgaca aagcaatgta cgaggaaccc tatagtggct tcgtgtcaga gatcatgaac
4321 gccgacgatc ggatcaagtt cagccaccag gtggataaga aaatgaatag gaaggtgagc
4381 gacgccacca tctacagtac tcgcaccggg aaactggcta aggataagaa agacgctgag
4441 tacatcgtgg caaaggtcaa agatatctac agcgtggacg gattcaagaa gttcaagaaa
4501 gtctacgata aggacaaaac caagtttctg ctgtacaaat atgatcctag gacattctca
4561 aagctggagc gcatcattag cgattgccca gacaaagtgg aaaaggtcca gacaaacggc
4621 aaagtgaagg ctgtcgatat cagtccattc gagatgtaca gaagggacca tgggatgatc
4681 aagaaatact caaagaaagg aaacggcccc gccatcaagc agctgaagta cctggataag
4741 aaactgggca gccacatcga cattaccccc gcaaacgcca atggaaaaca cgtgatcctg
4801 cagagtctga agccttggag aaccgacgtc tatctgaacc acgagacagg cgagtacgaa
4861 atcatgggga ttaagtatag cgatctgaag ttcaacaaga atgaggggta cggaatcaag
4921 aaagacaagt atctggaaat taagaaagtg gaggaagtct ccgagaagtc tgagttcatg
4981 tttagcctgt acaggaagga tcgcgtgaaa gtccaggaca tgaaaaccgg cgagtccgtg
5041 gaactgctgt tctggagcag gaacttttcc aataagaaat acgctgagct gaagcccatc
5101 tcccaggcag aaaacgacaa gaaactgcct gtgtatggca aagggagact gatcaagagg
5161 ctgattccca aaaactgtaa gatctggaaa gtgaatacca caattctggg cgatccctac
5221 tatctggaga aagaaagcga ctcccctaag gatatcctgg actaagaatt cggtaccgag
5281 ctcaagcttg cggccgccta tagtgtcacc taaatcgtaa cccagctttc ttgtacaaag
5341 tggtcgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
5401 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
5461 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
5521 tgacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
5581 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
5641 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
5701 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
5761 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
5821 ttctagcata accccttggg gcctctaaac gggtcttgag gggttttttg tctagaagac
5881 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
5941 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
6301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
6361 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
6421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
6481 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
6541 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
6601 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
6661 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
6721 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
6781 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
6841 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
6901 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
6961 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
7021 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
7081 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
7141 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
7201 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
7261 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
7321 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
7381 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
7441 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
7501 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
7561 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
7621 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
7681 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
7741 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
7801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
7861 tggtttgtcc aaactcatca atgtatctta tcatgtctgt tcatgaagcc ccttgagcat
7921 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
7981 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
8041 ttggtttaga gtttggcaac atatgcccat atgtaactag cataacccct tggggcctct
8101 aaacgggtct tgaggggttt tttgctgaaa gcatgcggag gaaattctcc ttgaagtttc
8161 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta
8221 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
8281 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
8341 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
8401 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
8461 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
8521 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
8581 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
8641 actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt
8701 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
8761 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
8821 tgtagttgag ctttttggaa ttgcgatcgc ataacttcgt atagcataca ttatacgaag
8881 ttataagctc ggaacgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
8941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
9001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
9061 gctccgcccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
9121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
9181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
9241 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
9301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
9361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
9421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
9481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
9541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
9601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gttaccaatg
9661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
9721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
9781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
9841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
9901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
9961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
10021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
10081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
10141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
10201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
10261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
10321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
10381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
10441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
10501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
10561 catgtccgcg cgtt
//
PX386载体其他相关CRISPR/Cas9载体：
