pWJ68
pWJ68
编号 | 载体名称 |
北京华越洋VECT75207 | pWJ68 |
pWJ68载体基本信息：
载体名称: | pwtCas9-bacteria |
质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
高拷贝/低拷贝: | 低拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | -- |
载体大小: | 9622 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | rpoZ (C ter) |
载体抗性: | Chloramphenicol |
克隆菌株: | Top10 |
宿主细胞（系）: | 细菌，如大肠杆菌 |
备注: | |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pWJ68载体质粒图谱和多克隆位点信息：
pWJ68载体序列：
ORIGIN
1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
1681 ATTACGAAAT CATCCTGTGG AGCTTAGTAG GTTTAGCAAG ATGGCAGCGC CTAAATGTAG
1741 AATGATAAAA GGATTAAGAG ATTAATTTCC CTAAAAATGA TAAAACAAGC GTTTTGAAAG
1801 CGCTTGTTTT TTTGGTTTGC AGTCAGAGTA GAATAGAAGT ATCAAAAAAA GCACCGACTC
1861 GGTGCCACTT TTTCAAGTTG ATAACGGACT AGCCTTATTT TAACTTGCTA TGCTGTTTTG
1921 AATGGTTCCA ACAAGATTAT TTTATAACTT TTATAACAAA TAATCAAGGA GAAATTCAAA
1981 GAAATTTATC AGCCATAAAA CAATACTTAA TACTATAGAA TGATAACAAA ATAAACTACT
2041 TTTTAAAAGA ATTTTGTGTT ATAATCTATT TATTATTAAG TATTGGGTAA TATTTTTTGA
2101 AGAGATATTT TGAAAAAGAA AAATTAAAGC ATATTAAACT AATTTCGGAG GTCATTAAAA
2161 CTATTATTGA ACAAATTGTT GGCAGACTGA ACCTGATTTC AGTATCATGC CCAGTCATTT
2221 CTTCACCTGT GGAGCTTTTT AAGTATGGCA CGCGTAACTG TTCAGGACGC TGTAGAGAAA
2281 ATTGGTAACC GTTTTGACCT GGTACTGGTC GCCGCGCGTC GCGCTCGTCA GATGCAGGTA
2341 GGCGGAAAGG ATCCGCTGGT ACCGGAAGAA AACGATAAAA CCACTGTAAT CGCGCTGCGC
2401 GAAATCGAAG AAGGTCTGAT CAACAACCAG ATCCTCGACG TTCGCGAACG CCAGGAACAG
2461 CAAGAGCAGG AAGCCGCTGA ATTACAAGCC GTTACCGCTA TTGCTGAAGG TCGTCGTGCT
2521 GCAGATAAGA AATACTCAAT AGGCTTAGCT ATCGGCACAA ATAGCGTCGG ATGGGCGGTG
2581 ATCACTGATG AATATAAGGT TCCGTCTAAA AAGTTCAAGG TTCTGGGAAA TACAGACCGC
2641 CACAGTATCA AAAAAAATCT TATAGGGGCT CTTTTATTTG ACAGTGGAGA GACAGCGGAA
2701 GCGACTCGTC TCAAACGGAC AGCTCGTAGA AGGTATACAC GTCGGAAGAA TCGTATTTGT
2761 TATCTACAGG AGATTTTTTC AAATGAGATG GCGAAAGTAG ATGATAGTTT CTTTCATCGA
2821 CTTGAAGAGT CTTTTTTGGT GGAAGAAGAC AAGAAGCATG AACGTCATCC TATTTTTGGA
2881 AATATAGTAG ATGAAGTTGC TTATCATGAG AAATATCCAA CTATCTATCA TCTGCGAAAA
2941 AAATTGGTAG ATTCTACTGA TAAAGCGGAT TTGCGCTTAA TCTATTTGGC CTTAGCGCAT
3001 ATGATTAAGT TTCGTGGTCA TTTTTTGATT GAGGGAGATT TAAATCCTGA TAATAGTGAT
3061 GTGGACAAAC TATTTATCCA GTTGGTACAA ACCTACAATC AATTATTTGA AGAAAACCCT
3121 ATTAACGCAA GTGGAGTAGA TGCTAAAGCG ATTCTTTCTG CACGATTGAG TAAATCAAGA
3181 CGATTAGAAA ATCTCATTGC TCAGCTCCCC GGTGAGAAGA AAAATGGCTT ATTTGGGAAT
3241 CTCATTGCTT TGTCATTGGG TTTGACCCCT AATTTTAAAT CAAATTTTGA TTTGGCAGAA
3301 GATGCTAAAT TACAGCTTTC AAAAGATACT TACGATGATG ATTTAGATAA TTTATTGGCG
3361 CAAATTGGAG ATCAATATGC TGATTTGTTT TTGGCAGCTA AGAATTTATC AGATGCTATT
3421 TTACTTTCAG ATATCCTAAG AGTAAATACT GAAATAACTA AGGCTCCCCT ATCAGCTTCA
3481 ATGATTAAAC GCTACGATGA ACATCATCAA GACTTGACTC TTTTAAAAGC TTTAGTTCGA
3541 CAACAACTTC CAGAAAAGTA TAAAGAAATC TTTTTTGATC AATCAAAAAA CGGATATGCA
3601 GGTTATATTG ATGGGGGAGC TAGCCAAGAA GAATTTTATA AATTTATCAA ACCAATTTTA
3661 GAAAAAATGG ATGGTACTGA GGAATTATTG GTGAAACTAA ATCGTGAAGA TTTGCTGCGC
3721 AAGCAACGGA CCTTTGACAA CGGCTCTATT CCCCATCAAA TTCACTTGGG TGAGCTGCAT
3781 GCTATTTTGA GAAGACAAGA AGACTTTTAT CCATTTTTAA AAGACAATCG TGAGAAGATT
3841 GAAAAAATCT TGACTTTTCG AATTCCTTAT TATGTTGGTC CATTGGCGCG TGGCAATAGT
3901 CGTTTTGCAT GGATGACTCG GAAGTCTGAA GAAACAATTA CCCCATGGAA TTTTGAAGAA
3961 GTTGTCGATA AAGGTGCTTC AGCTCAATCA TTTATTGAAC GCATGACAAA CTTTGATAAA
4021 AATCTTCCAA ATGAAAAAGT ACTACCAAAA CATAGTTTGC TTTATGAGTA TTTTACGGTT
4081 TATAACGAAT TGACAAAGGT CAAATATGTT ACTGAAGGAA TGCGAAAACC AGCATTTCTT
4141 TCAGGTGAAC AGAAGAAAGC CATTGTTGAT TTACTCTTCA AAACAAATCG AAAAGTAACC
4201 GTTAAGCAAT TAAAAGAAGA TTATTTCAAA AAAATAGAAT GTTTTGATAG TGTTGAAATT
4261 TCAGGAGTTG AAGATAGATT TAATGCTTCA TTAGGTACCT ACCATGATTT GCTAAAAATT
4321 ATTAAAGATA AAGATTTTTT GGATAATGAA GAAAATGAAG ATATCTTAGA GGATATTGTT
4381 TTAACATTGA CCTTATTTGA AGATAGGGAG ATGATTGAGG AAAGACTTAA AACATATGCT
4441 CACCTCTTTG ATGATAAGGT GATGAAACAG CTTAAACGTC GCCGTTATAC TGGTTGGGGA
4501 CGTTTGTCTC GAAAATTGAT TAATGGTATT AGGGATAAGC AATCTGGCAA AACAATATTA
4561 GATTTTTTGA AATCAGATGG TTTTGCCAAT CGCAATTTTA TGCAGCTGAT CCATGATGAT
4621 AGTTTGACAT TTAAAGAAGA CATTCAAAAA GCACAAGTGT CTGGACAAGG CGATAGTTTA
4681 CATGAACATA TTGCAAATTT AGCTGGTAGC CCTGCTATTA AAAAAGGTAT TTTACAGACT
4741 GTAAAAGTTG TTGATGAATT GGTCAAAGTA ATGGGGCGGC ATAAGCCAGA AAATATCGTT
4801 ATTGAAATGG CACGTGAAAA TCAGACAACT CAAAAGGGCC AGAAAAATTC GCGAGAGCGT
4861 ATGAAACGAA TCGAAGAAGG TATCAAAGAA TTAGGAAGTC AGATTCTTAA AGAGCATCCT
4921 GTTGAAAATA CTCAATTGCA AAATGAAAAG CTCTATCTCT ATTATCTCCA AAATGGAAGA
4981 GACATGTATG TGGACCAAGA ATTAGATATT AATCGTTTAA GTGATTATGA TGTCGATGCC
5041 ATTGTTCCAC AAAGTTTCCT TAAAGACGAT TCAATAGACA ATAAGGTCTT AACGCGTTCT
5101 GATAAAAATC GTGGTAAATC GGATAACGTT CCAAGTGAAG AAGTAGTCAA AAAGATGAAA
5161 AACTATTGGA GACAACTTCT AAACGCCAAG TTAATCACTC AACGTAAGTT TGATAATTTA
5221 ACGAAAGCTG AACGTGGAGG TTTGAGTGAA CTTGATAAAG CTGGTTTTAT CAAACGCCAA
5281 TTGGTTGAAA CTCGCCAAAT CACTAAGCAT GTGGCACAAA TTTTGGATAG TCGCATGAAT
5341 ACTAAATACG ATGAAAATGA TAAACTTATT CGAGAGGTTA AAGTGATTAC CTTAAAATCT
5401 AAATTAGTTT CTGACTTCCG AAAAGATTTC CAATTCTATA AAGTACGTGA GATTAACAAT
5461 TACCATCATG CCCATGATGC GTATCTAAAT GCCGTCGTTG GAACTGCTTT GATTAAGAAA
5521 TATCCAAAAC TTGAATCGGA GTTTGTCTAT GGTGATTATA AAGTTTATGA TGTTCGTAAA
5581 ATGATTGCTA AGTCTGAGCA AGAAATAGGC AAAGCAACCG CAAAATATTT CTTTTACTCT
5641 AATATCATGA ACTTCTTCAA AACAGAAATT ACACTTGCAA ATGGAGAGAT TCGCAAACGC
5701 CCTCTAATCG AAACTAATGG GGAAACTGGA GAAATTGTCT GGGATAAAGG GCGAGATTTT
5761 GCCACAGTGC GCAAAGTATT GTCCATGCCC CAAGTCAATA TTGTCAAGAA AACAGAAGTA
5821 CAGACAGGCG GATTCTCCAA GGAGTCAATT TTACCAAAAA GAAATTCGGA CAAGCTTATT
5881 GCTCGTAAAA AAGACTGGGA TCCAAAAAAA TATGGTGGTT TTGATAGTCC AACGGTAGCT
5941 TATTCAGTCC TAGTGGTTGC TAAGGTGGAA AAAGGGAAAT CGAAGAAGTT AAAATCCGTT
6001 AAAGAGTTAC TAGGGATCAC AATTATGGAA AGAAGTTCCT TTGAAAAAAA TCCGATTGAC
6061 TTTTTAGAAG CTAAAGGATA TAAGGAAGTT AAAAAAGACT TAATCATTAA ACTACCTAAA
6121 TATAGTCTTT TTGAGTTAGA AAACGGTCGT AAACGGATGC TGGCTAGTGC CGGAGAATTA
6181 CAAAAAGGAA ATGAGCTGGC TCTGCCAAGC AAATATGTGA ATTTTTTATA TTTAGCTAGT
6241 CATTATGAAA AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT GTTTGTGGAG
6301 CAGCATAAGC ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC TAAGCGTGTT
6361 ATTTTAGCAG ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA TAGAGACAAA
6421 CCAATACGTG AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA TCTTGGAGCT
6481 CCCGCTGCTT TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC GTCTACAAAA
6541 GAAGTTTTAG ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA AACACGCATT
6601 GATTTGAGTC AGCTAGGAGG TGACTGAAGT ATATTTTAGA TGAAGATTAT TTCTTAATAA
6661 CTAAAAATAT GGTATAATAC TCTTAATAAA TGCAGTAATA CAGGGGCTTT TCAAGACTGA
6721 AGTCTAGCTG AGACAAATAG TGCGATTACG AAATTTTTTA GACAAAAATA GTCTACGAGG
6781 TTTTAGAGCT ATGCTGTTTT GAATGGTCCC AAAACTGAGA CCAGTCTCGG AAGCTCAAAG
6841 GTCTCGTTTT AGAGCTATGC TGTTTTGAAT GGTCCCAAAA CTTCAGCACA CTGAGACTTG
6901 TTGAGTTCCA TGTTTTAGAG CTATGCTGTT TTGAATGGAC TCCATTCAAC ATTGCCGATG
6961 ATAACTTGAG AAAGAGGGTT AATACCAGCA GTCGGATACC TTCCTATTCT TTCTGTTAAA
7021 GCGTTTTCAT GTTATAATAG GCAAAAGAAG AGTAGTGTGA TCGTCCATTC CGACAGCATC
7081 GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC
7141 GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT
7201 GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CCTCTACGCC
7261 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
7321 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
7381 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
7441 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
7501 CAGGAGTCGC ATAAGGGAGA GCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC
7561 TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC
7621 ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA GGACCGCTTT
7681 CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC
7741 GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC
7801 GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG
7861 ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG
7921 GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG
7981 GCTCTTACCA GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC
8041 TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC
8101 CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC
8161 GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA
8221 CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA
8281 GCCGCACGCG GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC
8341 TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT
8401 CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA
8461 CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCCCCTA
8521 CGTGCTGCTG AAGTTGCCCG CAACAGAGAG TGGAACCAAC CGGTGATACC ACGATACTAT
8581 GACTGAGAGT CAACGCCATG AGCGGCCTCA TTTCTTATTC TGAGTTACAA CAGTCCGCAC
8641 CGCTGTCCGG TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA
8701 CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCCCAAC AGTCCCCCGG
8761 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCC CTGCACCATT ATGTTCCGGA
8821 TCTGCATCGC AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC
8881 GCTAACCGTT TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC
8941 CTCCACGGGG AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA CGGTCACACT
9001 GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC
9061 CTGCCCTGAA CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA
9121 TTATCACTTA TTCAGGCGTA GCACCAGGCG TTTAAGGGCA CCAATAACTG CCTTAAAAAA
9181 ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA
9241 CATGGAAGCC ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT
9301 CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG
9361 CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT
9421 TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG
9481 AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG
9541 TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT
9601 CACCGTCTTT CATTGCCATA CG
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pWJ68载体其他相关CRISPR/Cas9载体：
