pWJ66
pWJ66
编号 | 载体名称 |
北京华越洋VECT75206 | pWJ66 |
pWJ66载体基本信息：
载体名称: | pwtCas9-bacteria |
质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
高拷贝/低拷贝: | 低拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | -- |
载体大小: | 9552 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | rpoZ (C ter) |
载体抗性: | Chloramphenicol |
克隆菌株: | Top10 |
宿主细胞（系）: | 细菌，如大肠杆菌 |
备注: | |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pWJ66载体质粒图谱和多克隆位点信息：
pWJ66载体序列：
ORIGIN
1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
1681 ATTACGAAAT CATCCTGTGG AGCTTAGTAG GTTTAGCAAG ATGGCAGCGC CTAAATGTAG
1741 AATGATAAAA GGATTAAGAG ATTAATTTCC CTAAAAATGA TAAAACAAGC GTTTTGAAAG
1801 CGCTTGTTTT TTTGGTTTGC AGTCAGAGTA GAATAGAAGT ATCAAAAAAA GCACCGACTC
1861 GGTGCCACTT TTTCAAGTTG ATAACGGACT AGCCTTATTT TAACTTGCTA TGCTGTTTTG
1921 AATGGTTCCA ACAAGATTAT TTTATAACTT TTATAACAAA TAATCAAGGA GAAATTCAAA
1981 GAAATTTATC AGCCATAAAA CAATACTTAA TACTATAGAA TGATAACAAA ATAAACTACT
2041 TTTTAAAAGA ATTTTGTGTT ATAATCTATT TATTATTAAG TATTGGGTAA TATTTTTTGA
2101 AGAGATATTT TGAAAAAGAA AAATTAAAGC ATATTAAACT AATTTCGGAG GTCATTAAAA
2161 CTATTATTGA AATCATCAAA CTCATTATGG ATTTAATTTA AACTTTTTAT TTTAGGAGGC
2221 AAAAATGGAT AAGAAATACT CAATAGGCTT AGCTATCGGC ACAAATAGCG TCGGATGGGC
2281 GGTGATCACT GATGAATATA AGGTTCCGTC TAAAAAGTTC AAGGTTCTGG GAAATACAGA
2341 CCGCCACAGT ATCAAAAAAA ATCTTATAGG GGCTCTTTTA TTTGACAGTG GAGAGACAGC
2401 GGAAGCGACT CGTCTCAAAC GGACAGCTCG TAGAAGGTAT ACACGTCGGA AGAATCGTAT
2461 TTGTTATCTA CAGGAGATTT TTTCAAATGA GATGGCGAAA GTAGATGATA GTTTCTTTCA
2521 TCGACTTGAA GAGTCTTTTT TGGTGGAAGA AGACAAGAAG CATGAACGTC ATCCTATTTT
2581 TGGAAATATA GTAGATGAAG TTGCTTATCA TGAGAAATAT CCAACTATCT ATCATCTGCG
2641 AAAAAAATTG GTAGATTCTA CTGATAAAGC GGATTTGCGC TTAATCTATT TGGCCTTAGC
2701 GCATATGATT AAGTTTCGTG GTCATTTTTT GATTGAGGGA GATTTAAATC CTGATAATAG
2761 TGATGTGGAC AAACTATTTA TCCAGTTGGT ACAAACCTAC AATCAATTAT TTGAAGAAAA
2821 CCCTATTAAC GCAAGTGGAG TAGATGCTAA AGCGATTCTT TCTGCACGAT TGAGTAAATC
2881 AAGACGATTA GAAAATCTCA TTGCTCAGCT CCCCGGTGAG AAGAAAAATG GCTTATTTGG
2941 GAATCTCATT GCTTTGTCAT TGGGTTTGAC CCCTAATTTT AAATCAAATT TTGATTTGGC
3001 AGAAGATGCT AAATTACAGC TTTCAAAAGA TACTTACGAT GATGATTTAG ATAATTTATT
3061 GGCGCAAATT GGAGATCAAT ATGCTGATTT GTTTTTGGCA GCTAAGAATT TATCAGATGC
3121 TATTTTACTT TCAGATATCC TAAGAGTAAA TACTGAAATA ACTAAGGCTC CCCTATCAGC
3181 TTCAATGATT AAACGCTACG ATGAACATCA TCAAGACTTG ACTCTTTTAA AAGCTTTAGT
3241 TCGACAACAA CTTCCAGAAA AGTATAAAGA AATCTTTTTT GATCAATCAA AAAACGGATA
3301 TGCAGGTTAT ATTGATGGGG GAGCTAGCCA AGAAGAATTT TATAAATTTA TCAAACCAAT
3361 TTTAGAAAAA ATGGATGGTA CTGAGGAATT ATTGGTGAAA CTAAATCGTG AAGATTTGCT
3421 GCGCAAGCAA CGGACCTTTG ACAACGGCTC TATTCCCCAT CAAATTCACT TGGGTGAGCT
3481 GCATGCTATT TTGAGAAGAC AAGAAGACTT TTATCCATTT TTAAAAGACA ATCGTGAGAA
3541 GATTGAAAAA ATCTTGACTT TTCGAATTCC TTATTATGTT GGTCCATTGG CGCGTGGCAA
3601 TAGTCGTTTT GCATGGATGA CTCGGAAGTC TGAAGAAACA ATTACCCCAT GGAATTTTGA
3661 AGAAGTTGTC GATAAAGGTG CTTCAGCTCA ATCATTTATT GAACGCATGA CAAACTTTGA
3721 TAAAAATCTT CCAAATGAAA AAGTACTACC AAAACATAGT TTGCTTTATG AGTATTTTAC
3781 GGTTTATAAC GAATTGACAA AGGTCAAATA TGTTACTGAA GGAATGCGAA AACCAGCATT
3841 TCTTTCAGGT GAACAGAAGA AAGCCATTGT TGATTTACTC TTCAAAACAA ATCGAAAAGT
3901 AACCGTTAAG CAATTAAAAG AAGATTATTT CAAAAAAATA GAATGTTTTG ATAGTGTTGA
3961 AATTTCAGGA GTTGAAGATA GATTTAATGC TTCATTAGGT ACCTACCATG ATTTGCTAAA
4021 AATTATTAAA GATAAAGATT TTTTGGATAA TGAAGAAAAT GAAGATATCT TAGAGGATAT
4081 TGTTTTAACA TTGACCTTAT TTGAAGATAG GGAGATGATT GAGGAAAGAC TTAAAACATA
4141 TGCTCACCTC TTTGATGATA AGGTGATGAA ACAGCTTAAA CGTCGCCGTT ATACTGGTTG
4201 GGGACGTTTG TCTCGAAAAT TGATTAATGG TATTAGGGAT AAGCAATCTG GCAAAACAAT
4261 ATTAGATTTT TTGAAATCAG ATGGTTTTGC CAATCGCAAT TTTATGCAGC TGATCCATGA
4321 TGATAGTTTG ACATTTAAAG AAGACATTCA AAAAGCACAA GTGTCTGGAC AAGGCGATAG
4381 TTTACATGAA CATATTGCAA ATTTAGCTGG TAGCCCTGCT ATTAAAAAAG GTATTTTACA
4441 GACTGTAAAA GTTGTTGATG AATTGGTCAA AGTAATGGGG CGGCATAAGC CAGAAAATAT
4501 CGTTATTGAA ATGGCACGTG AAAATCAGAC AACTCAAAAG GGCCAGAAAA ATTCGCGAGA
4561 GCGTATGAAA CGAATCGAAG AAGGTATCAA AGAATTAGGA AGTCAGATTC TTAAAGAGCA
4621 TCCTGTTGAA AATACTCAAT TGCAAAATGA AAAGCTCTAT CTCTATTATC TCCAAAATGG
4681 AAGAGACATG TATGTGGACC AAGAATTAGA TATTAATCGT TTAAGTGATT ATGATGTCGA
4741 TGCCATTGTT CCACAAAGTT TCCTTAAAGA CGATTCAATA GACAATAAGG TCTTAACGCG
4801 TTCTGATAAA AATCGTGGTA AATCGGATAA CGTTCCAAGT GAAGAAGTAG TCAAAAAGAT
4861 GAAAAACTAT TGGAGACAAC TTCTAAACGC CAAGTTAATC ACTCAACGTA AGTTTGATAA
4921 TTTAACGAAA GCTGAACGTG GAGGTTTGAG TGAACTTGAT AAAGCTGGTT TTATCAAACG
4981 CCAATTGGTT GAAACTCGCC AAATCACTAA GCATGTGGCA CAAATTTTGG ATAGTCGCAT
5041 GAATACTAAA TACGATGAAA ATGATAAACT TATTCGAGAG GTTAAAGTGA TTACCTTAAA
5101 ATCTAAATTA GTTTCTGACT TCCGAAAAGA TTTCCAATTC TATAAAGTAC GTGAGATTAA
5161 CAATTACCAT CATGCCCATG ATGCGTATCT AAATGCCGTC GTTGGAACTG CTTTGATTAA
5221 GAAATATCCA AAACTTGAAT CGGAGTTTGT CTATGGTGAT TATAAAGTTT ATGATGTTCG
5281 TAAAATGATT GCTAAGTCTG AGCAAGAAAT AGGCAAAGCA ACCGCAAAAT ATTTCTTTTA
5341 CTCTAATATC ATGAACTTCT TCAAAACAGA AATTACACTT GCAAATGGAG AGATTCGCAA
5401 ACGCCCTCTA ATCGAAACTA ATGGGGAAAC TGGAGAAATT GTCTGGGATA AAGGGCGAGA
5461 TTTTGCCACA GTGCGCAAAG TATTGTCCAT GCCCCAAGTC AATATTGTCA AGAAAACAGA
5521 AGTACAGACA GGCGGATTCT CCAAGGAGTC AATTTTACCA AAAAGAAATT CGGACAAGCT
5581 TATTGCTCGT AAAAAAGACT GGGATCCAAA AAAATATGGT GGTTTTGATA GTCCAACGGT
5641 AGCTTATTCA GTCCTAGTGG TTGCTAAGGT GGAAAAAGGG AAATCGAAGA AGTTAAAATC
5701 CGTTAAAGAG TTACTAGGGA TCACAATTAT GGAAAGAAGT TCCTTTGAAA AAAATCCGAT
5761 TGACTTTTTA GAAGCTAAAG GATATAAGGA AGTTAAAAAA GACTTAATCA TTAAACTACC
5821 TAAATATAGT CTTTTTGAGT TAGAAAACGG TCGTAAACGG ATGCTGGCTA GTGCCGGAGA
5881 ATTACAAAAA GGAAATGAGC TGGCTCTGCC AAGCAAATAT GTGAATTTTT TATATTTAGC
5941 TAGTCATTAT GAAAAGTTGA AGGGTAGTCC AGAAGATAAC GAACAAAAAC AATTGTTTGT
6001 GGAGCAGCAT AAGCATTATT TAGATGAGAT TATTGAGCAA ATCAGTGAAT TTTCTAAGCG
6061 TGTTATTTTA GCAGATGCCA ATTTAGATAA AGTTCTTAGT GCATATAACA AACATAGAGA
6121 CAAACCAATA CGTGAACAAG CAGAAAATAT TATTCATTTA TTTACGTTGA CGAATCTTGG
6181 AGCTCCCGCT GCTTTTAAAT ATTTTGATAC AACAATTGAT CGTAAACGAT ATACGTCTAC
6241 AAAAGAAGTT TTAGATGCCA CTCTTATCCA TCAATCCATC ACTGGTCTTT ATGAAACACG
6301 CATTGATTTG AGTCAGCTAG GAGGTGACGC AGCTGCACGC GTAACTGTTC AGGACGCTGT
6361 AGAGAAAATT GGTAACCGTT TTGACCTGGT ACTGGTCGCC GCGCGTCGCG CTCGTCAGAT
6421 GCAGGTAGGC GGAAAGGATC CGCTGGTACC GGAAGAAAAC GATAAAACCA CTGTAATCGC
6481 GCTGCGCGAA ATCGAAGAAG GTCTGATCAA CAACCAGATC CTCGACGTTC GCGAACGCCA
6541 GGAACAGCAA GAGCAGGAAG CCGCTGAATT ACAAGCCGTT ACCGCTATTG CTGAAGGTCG
6601 TCGTTAATAC TCTTAATAAA TGCAGTAATA CAGGGGCTTT TCAAGACTGA AGTCTAGCTG
6661 AGACAAATAG TGCGATTACG AAATTTTTTA GACAAAAATA GTCTACGAGG TTTTAGAGCT
6721 ATGCTGTTTT GAATGGTCCC AAAACTGAGA CCAGTCTCGG AAGCTCAAAG GTCTCGTTTT
6781 AGAGCTATGC TGTTTTGAAT GGTCCCAAAA CTTCAGCACA CTGAGACTTG TTGAGTTCCA
6841 TGTTTTAGAG CTATGCTGTT TTGAATGGAC TCCATTCAAC ATTGCCGATG ATAACTTGAG
6901 AAAGAGGGTT AATACCAGCA GTCGGATACC TTCCTATTCT TTCTGTTAAA GCGTTTTCAT
6961 GTTATAATAG GCAAAAGAAG AGTAGTGTGA TCGTCCATTC CGACAGCATC GCCAGTCACT
7021 ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC GTTCTCGGAG
7081 CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT GGAGCCACTA
7141 TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CCTCTACGCC GGACGCATCG
7201 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG
7261 ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG
7321 TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG
7381 CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC
7441 ATAAGGGAGA GCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT
7501 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG
7561 TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG
7621 CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT
7681 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC GCCGGCATGG
7741 CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC
7801 CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT
7861 CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA
7921 GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA
7981 CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT
8041 TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC
8101 TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC
8161 GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG
8221 GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT
8281 GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG
8341 CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT
8401 GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCCCCTA CGTGCTGCTG
8461 AAGTTGCCCG CAACAGAGAG TGGAACCAAC CGGTGATACC ACGATACTAT GACTGAGAGT
8521 CAACGCCATG AGCGGCCTCA TTTCTTATTC TGAGTTACAA CAGTCCGCAC CGCTGTCCGG
8581 TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT
8641 TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCCCAAC AGTCCCCCGG CCACGGGGCC
8701 TGCCACCATA CCCACGCCGA AACAAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC
8761 AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTAACCGTT
8821 TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC CTCCACGGGG
8881 AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA CGGTCACACT GCTTCCGGTA
8941 GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC CTGCCCTGAA
9001 CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA
9061 TTCAGGCGTA GCACCAGGCG TTTAAGGGCA CCAATAACTG CCTTAAAAAA ATTACGCCCC
9121 GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC
9181 ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT
9241 ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA
9301 ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA
9361 CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG
9421 TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG
9481 CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT
9541 CATTGCCATA CG
//
pWJ66载体其他相关CRISPR/Cas9载体：
