pET-Cas9-NLS-6xHis
pET-Cas9-NLS-6xHis
编号 | 载体名称 |
北京华越洋VECT75211 | pET-Cas9-NLS-6xHis |
pET-Cas9-NLS-6xHis载体基本信息：
载体名称: | pET-Cas9-NLS-6xHis |
质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | lac/T7 |
载体大小: | 9265 bp |
5' 测序引物及序列: | T7 forward：5’TAATACGACTCACTATAGGG 3’ |
3' 测序引物及序列: | T7 Terminal：5’GCTAGTTATTGCTCAGCGG 3’ |
载体标签: | NLS 、 His tag (C-ter） |
载体抗性: | Ampicillin |
克隆菌株: | Mach1 |
宿主细胞（系）: | 细菌，如大肠杆菌 |
备注: | |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 非病毒 |
pET-Cas9-NLS-6xHis载体质粒图谱和多克隆位点信息：
pET-Cas9-NLS-6xHis载体序列：
ORIGIN
1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
61 gagatatacc atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg
121 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
181 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
241 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
301 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
361 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
421 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
481 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
541 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
601 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
661 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
721 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
781 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga
841 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa
901 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc
961 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct
1021 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc
1081 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa
1141 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa
1201 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga
1261 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg
1321 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg
1381 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg
1441 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa
1501 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa
1561 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta
1621 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc
1681 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg
1741 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag
1801 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt
1861 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga
1921 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa
1981 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac
2041 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa
2101 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat
2161 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg
2221 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat
2281 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga
2341 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc
2401 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa
2461 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca
2521 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga
2581 tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt
2641 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa
2701 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt
2761 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat
2821 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag
2881 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac
2941 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga
3001 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt
3061 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga
3121 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt
3181 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat
3241 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg
3301 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa
3361 aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga
3421 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc
3481 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt
3541 aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa
3601 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa
3661 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc
3721 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata
3781 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt
3841 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc
3901 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca
3961 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa
4021 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac
4081 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga
4141 aacacgcatt gatttgagtc agctaggagg tgaccccaag aagaagagga aggtgatgga
4201 taagcatcac caccaccatc actaatgata atttgaacgc cagcacatgg actcgtctac
4261 tagcgcagct taattaacct aggctgctgc caccgctgag caataactag cataacccct
4321 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
4381 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
4501 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4561 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
4621 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4681 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
4741 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
4801 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttctgg cggcacgatg
4861 gcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
4921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
4981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
5041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
5101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
5161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
5221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
5281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
5341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
5401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
5461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
5521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
5581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
5641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
5701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
5761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
5821 tactcttcct ttttcaatca tgattgaagc atttatcagg gttattgtct catgagcgga
5881 tacatatttg aatgtattta gaaaaataaa caaataggtc atgaccaaaa tcccttaacg
5941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6121 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
6181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
6241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
6301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
6361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
6421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
6481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
6541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
6601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
6661 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
6721 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
6781 ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca
6841 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
6901 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
6961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
7021 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
7081 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
7141 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
7201 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
7261 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
7321 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
7381 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
7441 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
7501 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
7561 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
7621 gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg ccccgcgccc
7681 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
7741 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
7861 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
7921 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
7981 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
8041 tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
8101 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
8161 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
8221 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
8281 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
8341 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
8401 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
8461 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
8521 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
8581 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
8641 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
8701 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
8761 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
8821 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
8881 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
8941 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
9001 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
9061 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
9121 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
9181 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
9241 cgcgaaatta atacgactca ctata
//
pET-Cas9-NLS-6xHis载体其他相关CRISPR/Cas9载体：
