北京华越洋生物
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首页    载体及质粒    pRetroX-TRE3G
pRetroX-TRE3Gvectormap

pRetroX-TRE3G

pRetroX-TRE3G

pRetroX-TRE3G

 



编号

载体名称

北京华越洋VECT55152

pRetroX-TRE3G

 

pRetroX-TRE3G逆病毒载体基本信息:

载体名称:

pRetroX-TRE3G

质粒类型:

逆病毒载体;四环素调控载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

TRE3GV

载体大小:

6600 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

载体抗性:

氨苄青霉素

筛选标记:

嘌呤霉素(Puromycin

克隆菌株:

DH5α

宿主细胞(系):

常规细胞系(293CV-1CHO等)

备注:

逆病毒载体pRetroX-TRE3G是四环素调控系统的应答载体,需要与相应的调控载体pRetroX-Tet3G共同使用。

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

逆转录病毒

 

pRetroX-TRE3G载体质粒图谱和多克隆位点信息:

  

 

pRetroX-TRE3G载体简介:

pRetroX-TRE3G载体序列:

ORIGIN

    1 TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC

   61 CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC

  121 GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA

  181 TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCTCTG GCATTATGCC

  241 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT

  301 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA

  361 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT

  421 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG

  481 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCAATAAA AGAGCCCACA ACCCCTCACT

  541 CGGCGCGCCA GTCTTCCGAT AGACTGCGTC GCCCGGGTAC CCGTATTCCC AATAAAGCCT

  601 CTTGCTGTTT GCATCCGAAT CGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA

  661 TTGACTACCC ACGACGGGGG TCTTTCATTT GGGGGCTCGT CCGGGATTTG GAGACCCCTG

  721 CCCAGGGACC ACCGACCCAC CACCGGGAGG TAAGCTGGCC AGCAACTTAT CTGTGTCTGT

  781 CCGATTGTCT AGTGTCTATG TTTGATGTTA TGCGCCTGCG TCTGTACTAG TTAGCTAACT

  841 AGCTCTGTAT CTGGCGGACC CGTGGTGGAA CTGACGAGTT CTGAACACCC GGCCGCAACC

  901 CTGGGAGACG TCCCAGGGAC TTTGGGGGCC GTTTTTGTGG CCCGACCTGA GGAAGGGAGT

  961 CGATGTGGAA TCCGACCCCG TCAGGATATG TGGTTCTGGT AGGAGACGAG AACCTAAAAC

 1021 AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC

 1081 TGCTGCAGCG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT

 1141 CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG

 1201 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT

 1261 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC

 1321 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG

 1381 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA

 1441 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC

 1501 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC

 1561 TAGGCGCCGG AATTGAAGAT CTCGATGAGG CCCTTTCGTC TTCACTCGAG TTTACTCCCT

 1621 ATCAGTGATA GAGAACGTAT GAAGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGCAG

 1681 ACTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGGAGTT TACTCCCTAT CAGTGATAGA

 1741 GAACGTATGA CCAGTTTACT CCCTATCAGT GATAGAGAAC GTATCTACAG TTTACTCCCT

 1801 ATCAGTGATA GAGAACGTAT ATCCAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG

 1861 AGGTAGGCGT GTACGGTGGG CGCCTATAAA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC

 1921 GCCTGGAGCA ATTCCACAAC ACTTTTGTCT TATACTTGGA TCCATCGATA CGCGTGCGGC

 1981 CGCGCCGGCG GGCCCGAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA

 2041 GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC

 2101 ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG

 2161 CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC

 2221 AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT

 2281 GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT

 2341 TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG

 2401 GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC

 2461 ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CAGCCCAAGC

 2521 TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG

 2581 CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC

 2641 CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC

 2701 TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC

 2761 CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA

 2821 GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA

 2881 AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC

 2941 TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT

 3001 TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA

 3061 CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG

 3121 CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT

 3181 CGGCGTCTCG AGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG

 3241 AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG

 3301 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC

 3361 AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGAACAGAT

 3421 GGAACAGGGT CGACCCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA

 3481 TGACCCTGTG CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT

 3541 TCTGCTCCCC GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG

 3601 ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC

 3661 TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG

 3721 GGTCTTTCAT TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC

 3781 ACCACCGGGA GGTAAGCTGG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA

 3841 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA

 3901 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA

 3961 CGTAGCGATA GCGGAGTGTA GATCCGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG

 4021 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC

 4081 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT

 4141 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT

 4201 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC

 4261 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT

 4321 TGCAAAAAGC TTACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC

 4381 ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT

 4441 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG

 4501 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA

 4561 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT

 4621 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC

 4681 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT

 4741 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG

 4801 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA

 4861 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT

 4921 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA

 4981 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA

 5041 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT

 5101 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT

 5161 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA

 5221 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA

 5281 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT

 5341 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG

 5401 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT

 5461 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG

 5521 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC

 5581 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG

 5641 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA

 5701 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA

 5761 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA

 5821 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA

 5881 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA

 5941 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG

 6001 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG

 6061 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG

 6121 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG

 6181 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC

 6241 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA

 6301 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG

 6361 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA

 6421 TCACGAGGCC CTTTCGTCTT CAAGAATTAG CTTGGCCATT GCATACGTTG TATCCATATC

 6481 ATAATATGTA CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT

 6541 TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT

//

pRetroX-TRE3G其他相关逆病毒载体:

pVSV-G

pBABE

pBABE-hygro-hTERT

pBABE-neo-SV40LT

pMKO.1-GFP

pBABE-neo

pBABE-GFP

pVPack-10A1

pBABE-Puro

pCMV-Gag-Pol

pVPack-Eco

pCMV-VSV-G

pCL-Eco

pCMV-VSV-G

pMSCV-PIG

pFB-ERV

pFB-hrGFP

pFB-Neo

pCFB

pRetroX-Tight-Pur-Luc

pRetroX-TetOne

pRetroX-PTuner2

pRetroX-PTuner-IRES

pRetroX-TRE3G-Luc

pRetroX-Tet-On     Advanced

pRetroQ-mCherry-N1

pRetroX-Tight-Pur

pRetroQ-AcGFP1-C1

pRetroQ-DsRed-Monomer-C1

RNAi-Ready     pSIREN-RetroQ-DsRed-Express

pRetroX-SG2M-Cyan

pQCXIX

pLXSN

RNAi-Ready     pSIREN-RetroQ-ZsGreen1

PSUPER.RETRO.PURO

pMSCVhyg

PRETROX-IRES-ZSGREEN1

pSuper.Retro.Neo

pMSCVpuro

pMKO.1-puro

pMSCV-FLIP-puro-dsRed-GFP-miRNA

pBABE-HAII

pBABE-puro-IRES-EGFP

pBABE-Puro     SV40LT

pBABE-neo-hTERT

pBABE-zeo

pBABE-hygro

pVPack-VSV-G

pVPack-GP

pCL-Ampho

pVPack-Ampho

pCMV-VSVG

pUMVC

pCFB-EGSH

pQCXIN

pFB

pFB-Luc

pRetroX-TetOne-luc

pRetro-Lib

pRetroX-PTuner2-C

pRetroX-PTuner

pRetroX-Tet3G

pRetroX-TRE3G

pRetroX-Tight-Hyg-Luc

pRetroX-Tet-Off     Advanced

pRetroQ-mCherry-C1

pRetroX-G1-Red

pRetroQ-AcGFP1-N1

pRetroQ-DsRed-Monomer-N1

pLXIN

pLNCX2

pSuper.Retro.Neo+GFP

pSilencer5.1-U6     Retro

pRetroX-IRES-DsRedExpress

RNAi-Ready     pSIREN-RetroQ

pMSCVneo

pQCXIH

pSilencer5.1-H1     Retro

pCLXSN