RNAi-Ready pSIREN-RetroQ-DsRed-Express
RNAi-Ready pSIREN-RetroQ-DsRed-Express
编号 | 载体名称 |
北京华越洋VECT55176 | RNAi-Ready pSIREN-RetroQ-DsRed-Express |
RNAi-Ready pSIREN-RetroQ-DsRed-Express逆病毒载体基本信息：
载体名称: | RNAi-Ready pSIREN-RetroQ-DsRed-Express |
质粒类型: | 哺乳动物细胞自失活基因沉默逆病毒载体，shRNA |
高拷贝/低拷贝: | 低拷贝 |
启动子: | Human U6 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 6543 bp |
5' 测序引物及序列: | U6-F: ATGGACTATCATATGCTTACCGTA (Clontech ) |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
备注: | 载体表达DsRed红色荧光蛋白作为基因沉默标志。 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
RNAi-Ready pSIREN-RetroQ-DsRed-Express载体质粒图谱和多克隆位点信息：
RNAi-Ready pSIREN-RetroQ-DsRed-Express载体简介
RNAi-Ready pSIREN-RetroQ-DsRed-Express载体序列：
ORIGIN
1 AATTCTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
61 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
121 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
181 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT
241 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC
301 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
361 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
421 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
481 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
541 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG
601 CGCTACCGGT CGCCACCATG GCCTCCTCCG AGGACGTCAT CAAGGAGTTC ATGCGCTTCA
661 AGGTGCGCAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG
721 GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGC GGCCCCCTGC
781 CCTTCGCCTG GGACATCCTG TCCCCCCAGT TCCAGTACGG CTCCAAGGTG TACGTGAAGC
841 ACCCCGCCGA CATCCCCGAC TACAAGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC
901 GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG
961 ACGGCTCCTT CATCTACAAG GTGAAGTTCA TCGGCGTGAA CTTCCCCTCC GACGGCCCCG
1021 TAATGCAGAA GAAGACTATG GGCTGGGAGG CCTCCACCGA GCGCCTGTAC CCCCGCGACG
1081 GCGTGCTGAA GGGCGAGATC CACAAGGCCC TGAAGCTGAA GGACGGCGGC CACTACCTGG
1141 TGGAGTTCAA GTCCATCTAC ATGGCCAAGA AGCCCGTGCA GCTGCCCGGC TACTACTACG
1201 TGGACTCCAA GCTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAGCAGTACG
1261 AGCGCGCCGA GGGCCGCCAC CACCTGTTCC TGTAGCTCGA GATATCAGTG GTCCAGGCTC
1321 TAGTTTTGAC TCAACAATAT CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAAAAT
1381 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG
1441 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG
1501 AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGGGTC GACCCTAGAG AACCATCAGA
1561 TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC
1621 AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC
1681 AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC
1741 CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC
1801 CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG
1861 GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GTAAGCTGGC TGCCTCGCGC
1921 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT
1981 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG
2041 GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAG ATCCGGCTGT
2101 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC
2161 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
2221 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC
2281 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA
2341 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT
2401 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TACTGGCTTA ACTATGCGGC
2461 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT
2521 AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
2581 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
2641 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
2701 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
2761 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
2821 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
2881 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
2941 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
3001 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
3061 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
3121 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
3181 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
3241 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
3301 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
3361 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
3421 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
3481 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
3541 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
3601 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
3661 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
3721 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
3781 GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
3841 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
3901 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
3961 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
4021 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4081 GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
4141 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
4201 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
4261 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
4321 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4381 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
4441 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
4501 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTAGC
4561 TTGGCCATTG CATACGTTGT ATCCATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
4621 AACATTACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
4681 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
4741 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
4801 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
4861 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA
4921 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG
4981 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT
5041 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
5101 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC
5161 CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC
5221 TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CTTCCGATAG ACTGCGTCGC
5281 CCGGGTACCC GTATTCCCAA TAAAGCCTCT TGCTGTTTGC ATCCGAATCG TGGTCTCGCT
5341 GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCAC GACGGGGGTC TTTCATTTGG
5401 GGGCTCGTCC GGGATTTGGA GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGTA
5461 AGCTGGCCAG CAACTTATCT GTGTCTGTCC GATTGTCTAG TGTCTATGTT TGATGTTATG
5521 CGCCTGCGTC TGTACTAGTT AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT
5581 GACGAGTTCT GAACACCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT TGGGGGCCGT
5641 TTTTGTGGCC CGACCTGAGG AAGGGAGTCG ATGTGGAATC CGACCCCGTC AGGATATGTG
5701 GTTCTGGTAG GAGACGAGAA CCTAAAACAG TTCCCGCCTC CGTCTGAATT TTTGCTTTCG
5761 GTTTGGAACC GAAGCCGCGC GTCTTGTCTG CTGCAGCGCT GCAGCATCGT TCTGTGTTGT
5821 CTCTGTCTGA CTGTGTTTCT GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC
5881 TTAAGTTTGA CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA
5941 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC
6001 GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC
6061 TTTTCACCTG GCCCGCATGG ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC
6121 TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT
6181 CTTCCTCCAT CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC
6241 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCGGAA TTGAAGATCT GGGCAGGAAG
6301 AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG ATACAAGGCT GTTAGAGAGA
6361 TAATTAGAAT TAATTTGACT GTAAACACAA AGATATTAGT ACAAAATACG TGACGTAGAA
6421 AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG TTTTAAAATG GACTATCATA
6481 TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC
6541 GAG
//
RNAi-Ready pSIREN-RetroQ-DsRed-Express其他相关逆病毒载体：
