RNAi-Ready pSIREN-RetroQ-ZsGreen1
RNAi-Ready pSIREN-RetroQ-ZsGreen1
编号 | 载体名称 |
北京华越洋VECT55178 | RNAi-Ready pSIREN-RetroQ-ZsGreen1 |
RNAi-Ready pSIREN-RetroQ-ZsGreen1逆病毒载体基本信息：
载体名称: | RNAi-Ready pSIREN-RetroQ-ZsGreen1 |
质粒类型: | 哺乳动物细胞自失活基因沉默逆病毒载体，shRNA |
高拷贝/低拷贝: | 低拷贝 |
启动子: | Human U6 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 6561 bp |
5' 测序引物及序列: | U6-F: ATGGACTATCATATGCTTACCGTA (Clontech ) |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | -- |
备注: | 载体在沉默基因 的同时表达ZsGreen1荧光蛋白。 |
稳定性: | / |
组成型: | 诱导型 |
病毒/非病毒: | 逆病毒 |
RNAi-Ready pSIREN-RetroQ-ZsGreen1载体质粒图谱和多克隆位点信息：
RNAi-Ready pSIREN-RetroQ-ZsGreen1载体简介
RNAi-Ready pSIREN-RetroQ-ZsGreen1载体序列：
ORIGIN
1 AATTCTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
61 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
121 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
181 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT
241 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC
301 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
361 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
421 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
481 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
541 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG
601 CGCTACCGGT CGCCACCATG GCCCAGTCCA AGCACGGCCT GACCAAGGAG ATGACCATGA
661 AGTACCGCAT GGAGGGCTGC GTGGACGGCC ACAAGTTCGT GATCACCGGC GAGGGCATCG
721 GCTACCCCTT CAAGGGCAAG CAGGCCATCA ACCTGTGCGT GGTGGAGGGC GGCCCCTTGC
781 CCTTCGCCGA GGACATCTTG TCCGCCGCCT TCATGTACGG CAACCGCGTG TTCACCGAGT
841 ACCCCCAGGA CATCGTCGAC TACTTCAAGA ACTCCTGCCC CGCCGGCTAC ACCTGGGACC
901 GCTCCTTCCT GTTCGAGGAC GGCGCCGTGT GCATCTGCAA CGCCGACATC ACCGTGAGCG
961 TGGAGGAGAA CTGCATGTAC CACGAGTCCA AGTTCTACGG CGTGAACTTC CCCGCCGACG
1021 GCCCCGTGAT GAAGAAGATG ACCGACAACT GGGAGCCCTC CTGCGAGAAG ATCATCCCCG
1081 TGCCCAAGCA GGGCATCTTG AAGGGCGACG TGAGCATGTA CCTGCTGCTG AAGGACGGTG
1141 GCCGCTTGCG CTGCCAGTTC GACACCGTGT ACAAGGCCAA GTCCGTGCCC CGCAAGATGC
1201 CCGACTGGCA CTTCATCCAG CACAAGCTGA CCCGCGAGGA CCGCAGCGAC GCCAAGAACC
1261 AGAAGTGGCA CCTGACCGAG CACGCCATCG CCTCCGGCTC CGCCTTGCCC TAACTCGAGA
1321 TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT
1381 ACGAGCCATA GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG
1441 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA
1501 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGGGTCGA
1561 CCCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT
1621 TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG
1681 CTCAATAAAA GAGCCCACAA CCCCTCACTC GGGGCGCCAG TCCTCCGATT GACTGAGTCG
1741 CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG TGGTCTCGCT
1801 GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTTCATTTG
1861 GGGGCTCGTC CGGGATCGGG AGACCCCTGC CCAGGGACCA CCGACCCACC ACCGGGAGGT
1921 AAGCTGGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
1981 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
2041 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG
2101 GAGTGTAGAT CCGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
2161 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
2221 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
2281 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
2341 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
2401 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA
2461 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA
2521 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT
2581 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
2641 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
2701 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
2761 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
2821 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
2881 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
2941 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
3001 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
3061 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
3121 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
3181 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
3241 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
3301 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
3361 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
3421 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
3481 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
3541 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
3601 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
3661 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
3721 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
3781 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT GCAGGCATCG TGGTGTCACG
3841 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
3901 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
3961 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
4021 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
4081 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ACACGGGATA ATACCGCGCC
4141 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
4201 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
4261 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
4321 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
4381 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
4441 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT
4501 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT
4561 TCGTCTTCAA GAATTAGCTT GGCCATTGCA TACGTTGTAT CCATATCATA ATATGTACAT
4621 TTATATTGGC TCATGTCCAA CATTACCGCC ATGTTGACAT TGATTATTGA CTAGTTATTA
4681 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA
4741 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT
4801 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA
4861 GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC
4921 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT
4981 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT
5041 GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG
5101 TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC
5161 AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA
5221 GGTCTATATA AGCAGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCT
5281 TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA AAGCCTCTTG CTGTTTGCAT
5341 CCGAATCGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCACGA
5401 CGGGGGTCTT TCATTTGGGG GCTCGTCCGG GATTTGGAGA CCCCTGCCCA GGGACCACCG
5461 ACCCACCACC GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG
5521 TCTATGTTTG ATGTTATGCG CCTGCGTCTG TACTAGTTAG CTAACTAGCT CTGTATCTGG
5581 CGGACCCGTG GTGGAACTGA CGAGTTCTGA ACACCCGGCC GCAACCCTGG GAGACGTCCC
5641 AGGGACTTTG GGGGCCGTTT TTGTGGCCCG ACCTGAGGAA GGGAGTCGAT GTGGAATCCG
5701 ACCCCGTCAG GATATGTGGT TCTGGTAGGA GACGAGAACC TAAAACAGTT CCCGCCTCCG
5761 TCTGAATTTT TGCTTTCGGT TTGGAACCGA AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC
5821 AGCATCGTTC TGTGTTGTCT CTGTCTGACT GTGTTTCTGT ATTTGTCTGA AAATTAGGGC
5881 CAGACTGTTA CCACTCCCTT AAGTTTGACC TTAGGTCACT GGAAAGATGT CGAGCGGATC
5941 GCTCACAACC AGTCGGTAGA TGTCAAGAAG AGACGTTGGG TTACCTTCTG CTCTGCAGAA
6001 TGGCCAACCT TTAACGTCGG ATGGCCGCGA GACGGCACCT TTAACCGAGA CCTCATCACC
6061 CAGGTTAAGA TCAAGGTCTT TTCACCTGGC CCGCATGGAC ACCCAGACCA GGTCCCCTAC
6121 ATCGTGACCT GGGAAGCCTT GGCTTTTGAC CCCCCTCCCT GGGTCAAGCC CTTTGTACAC
6181 CCTAAGCCTC CGCCTCCTCT TCCTCCATCC GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT
6241 TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC CTTCTCTAGG CGCCGGAATT
6301 GAAGATCTGG GCAGGAAGAG GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT
6361 ACAAGGCTGT TAGAGAGATA ATTAGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC
6421 AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT
6481 TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT
6541 ATATCTTGTG GAAAGGACGA G
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