北京华越洋生物
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首页    载体及质粒    pBABE-HAII
pBABE-HAIIMap

pBABE-HAII

pBABE-HAII

pBABE-HAII

 



编号

载体名称

北京华越洋VECT55182

pBABE-HAII

 

pBABE-HAII逆病毒载体基本信息:

载体名称:

pBABE-HAII

质粒类型:

逆病毒载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

MSCV

载体大小:

5206 bp

5' 测序引物及序列:

pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'

3' 测序引物及序列:

pBABE 3' primer   5'ACCCTAACTGACACACATTCC3'

载体标签:

载体抗性:

氨苄青霉素

筛选标记:

Puromycin

克隆菌株:

DH5αHB101

宿主细胞(系):

常规哺乳动物细胞系

备注:

 

推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
   

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

逆转录病毒

 

pBABE-HAII载体质粒图谱和多克隆位点信息:

 

pBABE-HAII载体简介

pBABE-HAII载体序列:

ORIGIN

    1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA

   61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT

  121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG

  181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC

  241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA

  301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC

  361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC

  421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT

  481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG

  541 TAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA

  601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA

  661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC

  721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG

  781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT

  841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT

  901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT

  961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC

 1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG

 1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA

 1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT

 1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT

 1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT

 1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCA ATGGCTTCAA GTTATCCATA

 1381 TGATGTGCCC GACTATGCCT CACTTGGAGG ACCCTCTGGA TCCGGAATTC TCCGTCGACC

 1441 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT

 1501 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA

 1561 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA

 1621 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA

 1681 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG

 1741 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTACCAT GACCGAGTAC

 1801 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC

 1861 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG

 1921 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG

 1981 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG

 2041 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC

 2101 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC

 2161 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG

 2221 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG

 2281 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGTGC

 2341 CCGAAGGACC GCGCGACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC

 2401 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGATAAA ATAAAAGATT

 2461 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA

 2521 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC

 2581 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA

 2641 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC

 2701 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG

 2761 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG

 2821 GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT

 2881 TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC

 2941 AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT

 3001 GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC

 3061 GTCAGCGGGG GTCTTTCATT TCCGACTTGT GGTCTCGCTG CCTTGGGAGG GTCTCCTCTG

 3121 AGTGATTGAC TACCCGTCAG CGGGGGTCTT CACATGCAGC ATGTATCAAA ATTAATTTGG

 3181 TTTTTTTTCT TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGAAT CGGCCAACGC

 3241 GCGGGGAGAG GCGGTTTGCG TATTGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

 3301 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT

 3361 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA

 3421 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC

 3481 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC

 3541 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

 3601 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA

 3661 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG

 3721 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC

 3781 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG

 3841 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT

 3901 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT

 3961 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC

 4021 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT

 4081 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT

 4141 AGATCCTTTT AAATTAAAAA TGAAGTTTGC GGCCGCAAAT CAATCTAAAG TATATATGAG

 4201 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT

 4261 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG

 4321 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA

 4381 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT

 4441 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA

 4501 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG

 4561 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC

 4621 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG

 4681 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA

 4741 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT

 4801 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC

 4861 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC

 4921 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA

 4981 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA

 5041 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT

 5101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA

 5161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC

//

 

pBABE-HAII其他相关逆病毒载体:

pVSV-G

pBABE

pBABE-hygro-hTERT

pBABE-neo-SV40LT

pMKO.1-GFP

pBABE-neo

pBABE-GFP

pVPack-10A1

pBABE-Puro

pCMV-Gag-Pol

pVPack-Eco

pCMV-VSV-G

pCL-Eco

pCMV-VSV-G

pMSCV-PIG

pFB-ERV

pFB-hrGFP

pFB-Neo

pCFB

pRetroX-Tight-Pur-Luc

pRetroX-TetOne

pRetroX-PTuner2

pRetroX-PTuner-IRES

pRetroX-TRE3G-Luc

pRetroX-Tet-On     Advanced

pRetroQ-mCherry-N1

pRetroX-Tight-Pur

pRetroQ-AcGFP1-C1

pRetroQ-DsRed-Monomer-C1

RNAi-Ready     pSIREN-RetroQ-DsRed-Express

pRetroX-SG2M-Cyan

pQCXIX

pLXSN

RNAi-Ready     pSIREN-RetroQ-ZsGreen1

PSUPER.RETRO.PURO

pMSCVhyg

PRETROX-IRES-ZSGREEN1

pSuper.Retro.Neo

pMSCVpuro

pMKO.1-puro

pMSCV-FLIP-puro-dsRed-GFP-miRNA

pBABE-HAII

pBABE-puro-IRES-EGFP

pBABE-Puro     SV40LT

pBABE-neo-hTERT

pBABE-zeo

pBABE-hygro

pVPack-VSV-G

pVPack-GP

pCL-Ampho

pVPack-Ampho

pCMV-VSVG

pUMVC

pCFB-EGSH

pQCXIN

pFB

pFB-Luc

pRetroX-TetOne-luc

pRetro-Lib

pRetroX-PTuner2-C

pRetroX-PTuner

pRetroX-Tet3G

pRetroX-TRE3G

pRetroX-Tight-Hyg-Luc

pRetroX-Tet-Off     Advanced

pRetroQ-mCherry-C1

pRetroX-G1-Red

pRetroQ-AcGFP1-N1

pRetroQ-DsRed-Monomer-N1

pLXIN

pLNCX2

pSuper.Retro.Neo+GFP

pSilencer5.1-U6     Retro

pRetroX-IRES-DsRedExpress

RNAi-Ready     pSIREN-RetroQ

pMSCVneo

pQCXIH

pSilencer5.1-H1     Retro

pCLXSN