pFB
pFB
编号 | 载体名称 |
北京华越洋VECT55189 | pFB |
pFB逆病毒载体基本信息：
载体名称: | pFB |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | -- |
载体大小: | 5117 bp |
5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 无 |
克隆菌株: | DH5α 等通用菌株 |
宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
pFB载体质粒图谱和多克隆位点信息：
pFB载体简介：
pFB载体描述
The Agilent pFB and pFB-Neo plasmid vectors for retroviral gene delivery and expression are derived from the Moloney murine leukemia virus (MMLV) and can be used to produce high titer viral stocks with MMLVbased packaging cell lines.1,2 Both vectors contain the bacterial origin of replication and ampicillin-resistance gene from pBR322, an extended MMLV packaging signal (ψ+), and a multiple cloning site (MCS) located between the MMLV 5′ and 3′ long terminal repeat sequences (LTRs).
The pFB vector is a minimal MMLV-based vector that can accommodate larger inserts than pFB-Neo. The pFB vector does not contain any exogenous sequence other than the sequence of the MCS.
The pFB-Neo vector differs from the pFB vector only by the presence of a cassette consisting of the internal ribosome entry site (IRES) from the encephalomyocarditis virus (EMCV) and the neomycin-resistance gene (neor). The open reading frame (ORF) for the neor gene is positioned downstream of the MCS and follows the IRES. A dicistronic message encoding the gene of interest and the neor gene is expressed from the viral promoter within the 5′ LTR.
pFB载体限制性酶切位点
pFB, 5117 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 2 978, 5043
Acc65I 3 647, 1846, 2643
AccI 3 1967, 2057, 3003
AflII 3 202, 1265, 2198
AflIII 2 164, 3232
AgeI 1 1840
AhdI 6 687, 733, 1274, 2683, 2729
4120
Alw44I 4 1054, 3048, 3546, 4792
AlwNI 5 323, 398, 2319, 2394, 3643
ApaI 1 1238
ApoI 3 87, 1128, 2063
AvaI 9 577, 610, 643, 1241, 2018
2074, 2573, 2606, 2639
BamHI 1 2069
BanII 7 580, 593, 764, 1238, 2576
2589, 2760
BbeI 3 615, 1656, 2611
BbsI 1 5110
BbvCI 2 453, 2449
BciVI 5 656, 1986, 2652, 3441, 4968
BglI 1 4239
BglII 1 1678
BpmI 3 1799, 2146, 4210
Bpu10I 7 337, 412, 453, 1548, 2333
2408, 2449
BsaAI 3 1816, 2000, 2984
BsaHI 6 615, 978, 1656, 2611, 4661
5043
BsaI 9 694, 715, 782, 1414, 1802
2690, 2711, 2778, 4192
BsaWI 5 138, 1840, 3438, 3585, 4416
BseRI 5 718, 1068, 1561, 1600, 2714
BsiEI 8 961, 1034, 1838, 2080, 3145
3569, 4492, 4641
BsiHKAI 7 580, 1054, 2576, 3048, 3546
4707, 4792
BsmBI 6 976, 1093, 1337, 1396, 1582
2881
Bsp120I 1 1238
BspHI 3 3952, 4960, 5065
BspLU11I 2 164, 3232
BsrBI 5 1299, 2077, 2102, 3163, 4964
BsrDI 2 4179, 4361
BsrGI 1 1541
BssHII 2 563, 2559
BssSI 3 3405, 4789, 5096
Bst1107I 1 3003
BstAPI 5 322, 397, 2318, 2393, 3050
BstEII 1 1346
BstF5I 9 682, 1386, 1573, 2031, 2678
2907, 4105, 4286, 4573
Bsu36I 1 1276
Cfr10I 2 1840, 4205
Csp6I 8 648, 895, 1542, 1819, 1847
2644, 3039, 4604
DraI 3 3989, 4008, 4700
DraIII 1 1873
DrdI 2 2921, 3334
DsaI 2 151, 931
EaeI 7 827, 961, 1368, 1389, 1668
2080, 4513
EarI 4 1334, 1564, 3110, 4914
Ecl136II 2 580, 2576
Eco52I 2 961, 2080
Eco57I 2 3759, 4807
Eco72I 1 2000
EcoNI 1 1647
EcoO109I 5 499, 1477, 1924, 2495, 5100
EcoRI 1 2063
EcoRV 4 308, 383, 2304, 2379
EheI 3 615, 1656, 2611
FokI 9 682, 1386, 1573, 2031, 2678
2907, 4105, 4286, 4573
FspI 1 4345
HaeII 5 615, 1656, 2611, 3106, 3476
HgaI 6 889, 1166, 2938, 3334, 3912
4662
HincII 2 2057, 4664
HinfI 10 637, 1020, 1045, 1863, 2108
2633, 3132, 3207, 3603, 4120
KasI 3 615, 1656, 2611
KpnI 3 647, 1846, 2643
MaeII 10 979, 1340, 1381, 1817, 2001
2985, 3935, 4351, 4724, 5044
MboII 10 1334, 1565, 2096, 3111, 3882
3973, 4728, 4806, 4915, 5111
MscI 3 827, 1368, 1668
MslI 4 159, 4373, 4532, 4891
MunI 2 11, 21
NarI 3 615, 1656, 2611
NdeI 3 1664, 1672, 3054
NheI 5 6, 16, 26, 197, 2193
NotI 1 2079
NspI 3 164, 2867, 3232
PleI 9 637, 1020, 1045, 1863, 2108
2633, 3132, 3603, 4120
PpuMI 4 499, 1477, 1924, 2495
PshAI 1 1015
Psp1406I 2 4350, 4723
PstI 3 1178, 1360, 4366
PvuI 2 1034, 4492
PvuII 4 286, 361, 2282, 2357
RsaI 8 648, 895, 1542, 1819, 1847
2644, 3039, 4604
SacI 2 580, 2576
SacII 1 151
SalI 1 2057
SapI 1 3109
ScaI 1 4603
SchI 9 637, 1020, 1045, 1863, 2108
2633, 3132, 3603, 4120
SexAI 1 1474
SfcI 7 182, 1178, 1360, 2178, 3497
3688, 4366
SmaI 4 643, 1241, 2018, 2639
SmlI 8 202, 1265, 2074, 2198, 3338
3600, 3877, 4745
SpeI 1 897
SrfI 1 1240
SspI 1 4927
StyI 5 496, 706, 1504, 2492, 2702
TaiI 10 979, 1340, 1381, 1817, 2001
2985, 3935, 4351, 4724, 5044
TaqI 9 1297, 1608, 1619, 2050, 2058
2075, 2119, 3332, 4776
TatI 3 1541, 3038, 4603
TfiI 1 3207
Tsp45I 6 1282, 1491, 2887, 2982, 4382
4593
TspRI 10 1284, 1875, 3128, 3634, 3647
3918, 4067, 4172, 4519, 4546
Tth111I 4 633, 1473, 2629, 2976
VspI 1 4296
XbaI 3 464, 2036, 2460
XhoI 1 2074
XhoII 8 1678, 2069, 3873, 3884, 3970
3982, 4750, 4767
XmaI 4 643, 1241, 2018, 2639
XmnI 1 4720
Enzymes that do NOT cut molecule:
AccIII AscI BclI BlnI Bpu1102I
BsaBI BsgI BsiWI BsmI BspMI
BstBI BstXI ClaI Eco47III FseI
HindIII HpaI MluI NaeI NcoI
NgoMIV NruI NsiI PacI PmeI
Ppu10I RsrII SanDI SbfI SfiI
SgfI SgrAI SnaBI SphI StuI
SwaI Van91I XcmI
pFB载体序列：
pFB, 5117 bp version 013001
NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been
obtained from existing data.
1 GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG
51 AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT
101 CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG
151 CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
201 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT
251 AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC
301 CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
351 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
401 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA
451 GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG
501 GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC
551 TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC
601 AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA
651 CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
701 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG
751 GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC
801 CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG
851 TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA
901 GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT
951 TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC
1001 CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT
1051 TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG
1101 AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA
1151 CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC
1201 TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG
1251 ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA
1301 GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA
1351 CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC
1401 GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC
1451 ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG
1501 AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT
1551 AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC
1601 TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT
1651 CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG
1701 CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC
1751 CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT
1801 GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC
1851 CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
1901 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC
1951 CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC
2001 ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT
2051 CGAATTGTCG ACGAATTCGG ATCCTCGAGC GGCCGCGATC CGAGTTCTTC
2101 TGAGCGGGAC TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA
2151 GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT
2201 AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAG
2251 AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA
2301 CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
2351 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC
2401 TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
2451 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC
2501 TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC
2551 TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC
2601 CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG
2651 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT
2701 TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT
2751 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC
2801 GACCCACCAC CGGGAGGTAA GCTGGCTGCC TCGCGCGTTT CGGTGATGAC
2851 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT
2901 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
2951 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA
3001 GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG
3051 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
3101 CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
3151 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
3201 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
3251 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
3301 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
3351 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
3401 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
3451 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
3501 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
3551 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
3601 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
3651 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
3701 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT
3751 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
3801 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
3851 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
3901 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
3951 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
4001 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
4051 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
4101 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
4151 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
4201 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
4251 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
4301 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA
4351 ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
4401 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
4451 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
4501 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
4551 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
4601 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT
4651 GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT
4701 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
4751 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
4801 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
4851 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
4901 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG
4951 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
5001 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA
5051 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
5101 GGCCCTTTCG TCTTCAA
pFB其他相关逆病毒载体：
