pRSV-rev
pRSV-rev
编号 | 载体名称 |
北京华越洋VECT231343 | pRSV-rev |
pRSV-rev载体基本信息：
载体名称: | pRSV-rev |
质粒类型: | 慢病毒包装载体；哺乳动物表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | RSV U3 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 4174bp |
5' 测序引物及序列: | pREP fwd: GCTCGATACAATAAACGCC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素（Ampicillian） |
筛选标记: | -- |
备注: | pRSV-rev第三代慢病毒包装载体，与之配套的包装载体为pMD2.G、pMDLg/pRRE。 |
稳定性: | 瞬表达 |
组成型: | 组成型 |
病毒/非病毒: | 慢病毒 |
pRSV-rev载体质粒图谱和多克隆位点信息：
pRSV-rev载体简介：
Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system.
pRSV-rev 载体序列：
ORIGIN9
1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GAATTCGATG
241 TACGGGCCAG ATATACGCGT ATCTGAGGGG ACTAGGTGTG TTTAGGCGAA AAGCGGGGCT
301 TCGGTTGTAC GCGGTTAGGA GTCCCCTCAG GATTAGTAGT TTCGCTTTTG CATAGGGAGG
361 GGGAAATGTA GTCTTATGCA ATACACTTGT AGTCTTGCAA CATGGTAACG ATGAGTTAGC
421 AACATGCCTT ACAAGGAGAG AAAAAGCACC GTGCATGCCG ATTGGTGGAA GTAAGGTGGT
481 ACGATCGTGC CTTATTAGGA AGGCAACAGA CAGGTCTGAC ATGGATTGGA CGAACCACTG
541 AATTCCGCAT TGCAGAGATA ATTGTATTTA AGTGCCTAGC TCGATACAAT AAACGCCATT
601 TGACCATTCA CCACATTGGT GTGCACCTCC AAGCTCGAGC TCGTTTAGTG AACCGTCAGA
661 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
721 GCCTCCCCTC GAAGCTAGTC GATTAGGCAT CTCCTATGGC AGGAAGAAGC GGAGACAGCG
781 ACGAAGACCT CCTCAAGGCA GTCAGACTCA TCAAGTTTCT CTATCAAAGC AACCCACCTC
841 CCAATCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC
901 AGAGACAGAT CCATTCGATT AGTGAACGGA TCCTTAGCAC TTATCTGGGA CGATCTGCGG
961 AGCCTGTGCC TCTTCAGCTA CCACCGCTTG AGAGACTTAC TCTTGATTGT AACGAGGATT
1021 GTGGAACTTC TGGGACGCAG GGGGTGGGAA GCCCTCAAAT ATTGGTGGAA TCTCCTACAA
1081 TATTGGAGTC AGGAGCTAAA GAATAGTGCT GTTAGCTTGC TCAATGCCAC AGCTATAGCA
1141 GTAGCTGAGG GGACAGATAG GGTTATAGAA GTAGTACAAG AAGCTTGGCA CTGGCCGTCG
1201 TTTTACATGA TCTGAGCCTG GGAGATCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT
1261 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT
1321 AACTAGAGAT CAGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC
1381 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG
1441 CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT
1501 TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG
1561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
1621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA
1681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA
1741 CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT
1801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC
1861 AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
1921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT
1981 ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
2041 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
2101 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
2161 CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG
2221 ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT
2281 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA
2341 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC
2401 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG
2461 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC
2521 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT
2581 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC
2641 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG
2701 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT
2761 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT
2821 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA
2881 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC
2941 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG
3001 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT
3061 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA
3121 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT
3181 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA
3241 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
3301 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
3361 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
3421 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
3481 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
3541 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
3601 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
3661 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
3721 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
3781 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
3841 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
3901 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
3961 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
4021 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
4081 GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC
4141 GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAG
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