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pMHE3Tc

pMHE3Tc

pMHE3Tc



 

编号

载体名称

北京华越洋VECT75101

pMHE3Tc

 

pMHE3Tc载体基本信息:

载体名称:

pMHE3Tc

质粒类型:

广宿主载体;细菌表达载体;体外表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

6384 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

T7 tagN-ter);6xHis tag  (C-ter)

载体抗性:

四环素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE3Tc载体质粒图谱和多克隆位点信息:


 

pMHE3Tc载体序列:

       ORIGIN      

        1 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca

       61 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag

      121 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga

      181 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc

      241 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagcatgact

      301 ggtggacagc aaatgggtcg ggatccgaat tcgagctccg tcgacaagct tgcggccgca

      361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct

      421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg

      481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc

      541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac

      601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg

      661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt

      721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc

      781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct

      841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga

      901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga

      961 attttaacaa aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt

     1021 taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt aaggcagtta

     1081 ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc

     1141 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg

     1201 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct

     1261 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata

     1321 tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta gcgaggtgcc

     1381 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag

     1441 gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca gcgcacttac

     1501 gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc ggcggctcca

     1561 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc

     1621 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc

     1681 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca

     1741 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat

     1801 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg

     1861 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg

     1921 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc

     1981 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg

     2041 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg

     2101 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc

     2161 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc gcacggttgc

     2221 ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta

     2281 aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc

     2341 cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt

     2401 tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg

     2461 ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg

     2521 cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc

     2581 gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact cgcataggct

     2641 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc

     2701 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt

     2761 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg

     2821 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg

     2881 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag

     2941 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag

     3001 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc gccgtaggcg

     3061 gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg gcccgccgga

     3121 ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag gggcaggcat

     3181 ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg tggcggccag

     3241 tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca ggacgccaga

     3301 gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc gcgagttgct

     3361 gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga cggccagccc

     3421 ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga aggcgcacaa

     3481 gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc accgtgacga

     3541 aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca ggctgtcggc

     3601 caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt ttgcggccgc

     3661 tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc acacgcgcat

     3721 tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca tcagcccgca

     3781 agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg gaatctcaaa

     3841 gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc ggcaggggta

     3901 tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg ccgatcaagc

     3961 ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg ccctggggcc

     4021 gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg gcgaaaagct

     4081 gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc aggaacgcgg

     4141 gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta ccgaacaact

     4201 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga

     4261 tagctttatg cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct

     4321 agggtcccca attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg

     4381 gatcaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga

     4441 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg

     4501 acccgcagtt gcaaaccctc actgatccgc cgagatgcgc cgcgtgcggc tgctggagat

     4561 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa

     4621 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc

     4681 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg

     4741 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa

     4801 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat

     4861 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg

     4921 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc

     4981 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc

     5041 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc

     5101 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc

     5161 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag

     5221 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg

     5281 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag

     5341 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag

     5401 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa

     5461 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag

     5521 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg

     5581 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa

     5641 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat

     5701 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg

     5761 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca

     5821 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt

     5881 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctc gcgcagatca

     5941 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg

     6001 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg

     6061 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt

     6121 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc

     6181 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct

     6241 ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat

     6301 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta

     6361 caagcataaa gctcaaggag atgg

//

 

 

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