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pMHE5Tc

pMHE5Tc

pMHE5Tc

 



编号

载体名称

北京华越洋VECT75102

pMHE5Tc

 

pMHE5Tc载体基本信息:

载体名称:

pMHE5Tc

质粒类型:

广宿主载体;细菌表达载体;体外表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

6032 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

6XHis tagN-ter);FLAG-tagStrep-tag II (C-ter)

载体抗性:

四环素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE5Tc载体质粒图谱和多克隆位点信息:

 

pMHE5Tc载体序列:

       ORIGIN      

        1 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag

       61 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct

      121 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg

      181 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa

      241 taattttgtt taactttaag aaggagatat accatggggc atcatcatca tcatcatatc

      301 gagggaaggc ctgtcgacgg tatcgataag cttgatatcg aattcctgca gctcgaggga

      361 tccgactaca aggacgacga cgacaagaac tggagccatc cgcagttcga gaagtgaaga

      421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata

      481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg

      541 aactatatcc ggatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt

      601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa

      661 ccaataggcc gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg

      721 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga

      781 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt

      841 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct

      901 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga

      961 tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc

     1021 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca

     1081 gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg

     1141 ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg

     1201 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc

     1261 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg

     1321 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg

     1381 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa

     1441 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca

     1501 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga

     1561 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga

     1621 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca

     1681 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct

     1741 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga

     1801 tttctctgga ctgcgtggcc atgcttatct ccatgcggta gggtgccgca cggttgcggc

     1861 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa

     1921 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc

     1981 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg

     2041 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct

     2101 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc

     2161 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg

     2221 aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc ccccactcgc ataggcttgg

     2281 gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc

     2341 cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc

     2401 ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg

     2461 ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag

     2521 gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca

     2581 gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga

     2641 ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc gtaggcggtc

     2701 acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc cgccggaggc

     2761 accgccttgc gctgcccccg tcgagccggt tggacaccaa aagggagggg caggcatggc

     2821 ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg cggccagtct

     2881 caagcacgcc taccgcgagc gcgagacgcc caacgctgac gccagcagga cgccagagaa

     2941 cgagcactgg gcggccagca gcaccgatga agcgatgggc cgactgcgcg agttgctgcc

     3001 agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg ccagcccgga

     3061 atggtggaag tcggccagcc aagaacagca ggcggcgttc ttcgagaagg cgcacaagtg

     3121 gctggcggac aagtacgggg cggatcgcat cgtgacggcc agcatccacc gtgacgaaac

     3181 cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc tgtcggccaa

     3241 ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag accacgtttg cggccgctgt

     3301 ggccgatcta gggctgcaac ggggcatcga gggcagcaag gcacgtcaca cgcgcattca

     3361 ggcgttctac gaggccctgg agcggccacc agtgggccac gtcaccatca gcccgcaagc

     3421 ggtcgagcca cgcgcctatg caccgcaggg attggccgaa aagctgggaa tctcaaagcg

     3481 cgttgagacg ccggaagccg tggccgaccg gctgacaaaa gcggttcggc aggggtatga

     3541 gcctgcccta caggccgccg caggagcgcg tgagatgcgc aagaaggccg atcaagccca

     3601 agagacggcc cgagaccttc gggagcgcct gaagcccgtt ctggacgccc tggggccgtt

     3661 gaatcgggat atgcaggcca aggccgccgc gatcatcaag gccgtgggcg aaaagctgct

     3721 gacggaacag cgggaagtcc agcgccagaa acaggcccag cgccagcagg aacgcgggcg

     3781 cgcacatttc cccgaaaagt gccacctggc ggcgttgtga caatttaccg aacaactccg

     3841 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatag

     3901 ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg

     3961 gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca tccaccggat

     4021 caattcccct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc

     4081 ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc

     4141 cgcagttgca aaccctcact gatccgccga gatgcgccgc gtgcggctgc tggagatggc

     4201 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa

     4261 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt

     4321 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat

     4381 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc

     4441 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct

     4501 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc

     4561 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc

     4621 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc

     4681 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag

     4741 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg

     4801 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca

     4861 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg

     4921 aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag

     4981 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag

     5041 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg

     5101 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg

     5161 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc

     5221 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg

     5281 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga

     5341 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa

     5401 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca

     5461 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc

     5521 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctcgcg cagatcagtt

     5581 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct

     5641 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac

     5701 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag

     5761 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg

     5821 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta

     5881 ctaagctgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa

     5941 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa

     6001 gcataaagct caaggagatg gcgcccaaca gt

//

 

 

 

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