pPRIME-TET-GFP-FF3
pPRIME-TET-GFP-FF3
编号 | 载体名称 |
北京华越洋VECT231252 | pPRIME-TET-GFP-FF3 |
pPRIME-TET-GFP-FF3载体基本信息：
载体名称: | pPRIME-TET-GFP-FF3 |
质粒类型: | 慢病毒载体；RNAi载体；四环素诱导载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | TET |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 8226bp |
5' 测序引物及序列: | EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG |
3' 测序引物及序列: | -- |
载体标签: | GFP |
载体抗性: | 氨苄青霉素 |
筛选标记: | 氯霉素 |
备注: | 克隆宿主菌为Stbl3，在30度条件下进行质粒扩增。 |
稳定性: | 稳表达 |
组成型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
pPRIME-TET-GFP-FF3载体质粒图谱和多克隆位点信息：
pPRIME-TET-GFP-FF3载体序列：
ORIGIN
1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA GACCAGATCT GAGCCTGGGA GCTCTCTGGC
841 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG
901 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG
961 TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG
1021 AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC
1081 GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG
1141 AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC
1201 CAGGGGGAAA GAAAAAATAT AAATAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA
1261 TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG
1321 CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA
1381 ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG
1441 ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCGCTGAT
1501 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
1561 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
1621 GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG
1681 AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG
1741 TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA
1801 ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT
1861 AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTCATTT GCACCACTGC
1921 TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC
1981 CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA
2041 AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC
2101 AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT
2161 GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG
2221 AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC
2281 CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG
2341 ATTAGTGAAC GGATCGGCAC TGCGTGCGCC AATTCTGCAG ACAAATGGCA GTATTCATCC
2401 ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA
2461 TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT
2521 TTCGGGTTTA TTACAGGGAC AGCAGAGATC CAGTTTGGTT AGTACCGGGC CCGCTCTAGA
2581 CCTTTCGTCT TCATTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGTTTAC
2641 TCCCTATCAG TGATAGAGAA CGATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT
2701 GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA
2761 TAGAGAACGT ATGTCGAGTT TATCCCTATC AGTGATAGAG AACGTATGTC GAGTTTACTC
2821 CCTATCAGTG ATAGAGAACG TATGTCGAGG TAGGCGTGTA CGGTGGGAGG CCTATATAAG
2881 CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGACCGGT CGCCACCATG GTGAGCAAGG
2941 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
3001 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
3061 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
3121 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
3181 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
3241 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
3301 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
3361 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
3421 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
3481 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
3541 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
3601 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAGCGG CCGCAAGCCT
3661 TGTTAAGTGC TCGCTTCGGC AGCACATATA CTATGTTTGA ATGAGGCTTC AGTACTTTAC
3721 AGAATCGTTG CCTGCACATC TTGGAAACAC TTGCTGGGAT TACTTCTTCA GGTTAACCCA
3781 ACAGAAGGCT CGAGCAACCA GAATTCAAGG GGCTACTTTA GGAGCAATTA TCTTGTTTAC
3841 TAAAACTGAA TACCTTGCTA TCTCTTTGAT ACATTTTTAC AAAGCTGAAT TAAAATGGTA
3901 TAAATTAAAT CACTTTTTTC AATTGGAAGA CTAATGCGTT TAAACACGCG GCGACGCGTT
3961 CGACCGAATA AAACCTGTGA CGGAAGATCA CTTCGCAGAA TAAATAAATC CTGGTGTCCC
4021 TGTTGATACC GGGAAGCCCT GGGCCAACTT TTGGCGAAAA TGAGACGTTG ATCGGCACGT
4081 AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT
4141 GTCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC
4201 CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA
4261 ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA
4321 AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA
4381 TCCGGAATTA CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC
4441 TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA
4501 CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA
4561 CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG
4621 GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT
4681 TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA
4741 GGTTCATCAT GCCGTTTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA
4801 GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA
4861 AACGCCTGGT TGCTACGCCT GAATAAGTGA TAATAAGCGG ATGAATGGCA GAAATTCGGA
4921 TCTCGACCGC GTTTGGGCGG TGGCTCCCTG CCACGCGGCT CCGAACAGAA GCTGATCTCC
4981 GAAGAGGATC TGACATGTGT TTAAACCTCG ACTTAATTAA GTCGAGGGTC GACGGTATCG
5041 ATAAGCTCGC TTCACGAGAT CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA
5101 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC
5161 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG
5221 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC
5281 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG
5341 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG
5401 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA
5461 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT
5521 TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG
5581 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC
5641 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC
5701 CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC
5761 AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG
5821 GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT
5881 CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA
5941 CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC
6001 TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA
6061 CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC
6121 CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC
6181 AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC
6241 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG
6301 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT
6361 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA
6421 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
6481 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
6541 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
6601 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
6661 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
6721 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
6781 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
6841 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
6901 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
6961 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
7021 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
7081 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
7141 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
7201 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
7261 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
7321 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
7381 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
7441 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
7501 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
7561 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
7621 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
7681 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
7741 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
7801 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
7861 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
7921 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
7981 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
8041 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
8221 CCTGAC
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pPRIME-TET-GFP-FF3其他相关慢病毒载体：
