pLOX-CW-CRE
pLOX-CW-CRE
编号 | 载体名称 |
北京华越洋VECT231254 | pLOX-CW-CRE |
pLOX-CW-CRE载体基本信息：
载体名称: | pLOX-CW-CRE |
质粒类型: | 慢病毒系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 9886bp |
5' 测序引物及序列: | CMV forward : CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | SP6 : ATTTAGGTGACACTATAG |
载体标签: | -- |
载体抗性: | 氨苄青霉素（Ampicillian） |
筛选标记: | -- |
备注: | 克隆菌株为 Stbl3或HB101。 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pLOX-CW-CRE载体质粒图谱和多克隆位点信息：
pLOX-CW-CRE载体序列：
ORIGIN
1 AGTTGGAAGG GCTAATTCAC TCCCAAAGAA GACAAGATAT CCTTGATCTG TGGATCTACC
61 ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC
121 CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG
181 CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC
241 CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC
301 GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT
361 CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA
421 GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC
481 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG
541 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC
601 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA
661 AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA
721 CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT
781 AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT
841 GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT
901 GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG
961 GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA
1021 GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG
1081 ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC
1141 AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT
1201 GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA
1261 AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG
1321 TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC
1381 AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG
1441 CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG
1501 GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA
1561 CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG
1621 ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA
1681 ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG
1741 GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT
1801 ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA
1861 CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC
1921 CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC
1981 AGAGACAGAT CCATTCGATT AGTGAACGGA TCTCGACGGT ATCGATAAGC TTGGGAGTTC
2041 CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA
2101 TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT
2161 CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG
2221 CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG
2281 TACATGACCT TACGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT
2341 ACCATGGTGA TGCGGTTTTG GCAGTACACC AATGGGCGTG GATAGCGGTT TGACTCACGG
2401 GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA
2461 CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT
2521 GTACGGTGGG AGGTCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA
2581 CGCCATCCAC GCTGTTTTGA CCTCCATAGA AGACACCGAC TCTAGCTAGA GGATCTACCA
2641 TGCCCAAGAA GAAGAGGAAG GTGTCCAATT TACTGACCGT ACACCAAAAT TTGCCTGCAT
2701 TACCGGTCGA TGCAACGAGT GATGAGGTTC GCAAGAACCT GATGGACATG TTCAGGGATC
2761 GCCAGGCGTT TTCTGAGCAT ACCTGGAAAA TGCTTCTGTC CGTTTGCCGG TCGTGGGCGG
2821 CATGGTGCAA GTTGAATAAC CGGAAATGGT TTCCCGCAGA ACCTGAAGAT GTTCGCGATT
2881 ATCTTCTATA TCTTCAGGCG CGCGGTCTGG CAGTAAAAAC TATCCAGCAA CATTTGGGCC
2941 AGCTAAACAT GCTTCATCGT CGGTCCGGGC TGCCACGACC AAGTGACAGC AATGCTGTTT
3001 CACTGGTTAT GCGGCGGATC CGAAAAGAAA ACGTTGATGC CGGTGAACGT GCAAAACAGG
3061 CTCTAGCGTT CGAACGCACT GATTTCGACC AGGTTCGTTC ACTCATGGAA AATAGCGATC
3121 GCTGCCAGGA TATACGTAAT CTGGCATTTC TGGGGATTGC TTATAACACC CTGTTACGTA
3181 TAGCCGAAAT TGCCAGGATC AGGGTTAAAG ATATCTCACG TACTGACGGT GGGAGAATGT
3241 TAATCCATAT TGGCAGAACG AAAACGCTGG TTAGCACCGC AGGTGTAGAG AAGGCACTTA
3301 GCCTGGGGGT AACTAAACTG GTCGAGCGAT GGATTTCCGT CTCTGGTGTA GCTGATGATC
3361 CGAATAACTA CCTGTTTTGC CGGGTCAGAA AAAATGGTGT TGCCGCGCCA TCTGCCACCA
3421 GCCAGCTATC AACTCGCGCC CTGGAAGGGA TTTTTGAAGC AACTCATCGA TTGATTTACG
3481 GCGCTAAGGA TGACTCTGGT CAGAGATACC TGGCCTGGTC TGGACACAGT GCCCGTGTCG
3541 GAGCCGCGCG AGATATGGCC CGCGCTGGAG TTTCAATACC GGAGATCATG CAAGCTGGTG
3601 GCTGGACCAA TGTAAATATT GTCATGAACT ATATCCGTAA CCTGGATAGT GAAACAGGGG
3661 CAATGGTGCG CCTGCTGGAA GATGGCGATT AGGTCGAGCT CAAGCTTCGA ATTCGATAAT
3721 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT
3781 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG
3841 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG
3901 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT
3961 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT
4021 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG
4081 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC
4141 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT
4201 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC
4261 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG GGAATTCGAG
4321 CTCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA
4381 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATG GGATCAATTC
4441 ACCATGGGAA TAACTTCGTA TAGCATACAT TATACGAAGT TATGCTGCTT TTTGCTTGTA
4501 CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC
4561 CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT
4621 TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA
4681 GCAGCATCTA GAATTAATTC CGTGTATTCT ATAGTGTCAC CTAAATCGTA TGTGTATGAT
4741 ACATAAGGTT ATGTATTAAT TGTAGCCGCG TTCTAACGAC AATATGTACA AGCCTAATTG
4801 TGTAGCATCT GGCTTACTGA AGCAGACCCT ATCATCTCTC TCGTAAACTG CCGTCAGAGT
4861 CGGTTTGGTT GGACGAACCT TCTGAGTTTC TGGTAACGCC GTCCCGCACC CGGAAATGGT
4921 CAGCGAACCA ATCAGCAGGG TCATCGCTAG CCAGATCCTC TACGCCGGAC GCATCGTGGC
4981 CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG
5041 GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC
5101 AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC
5161 GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA
5221 GGGAGAGCGT CGAATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC
5281 AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT
5341 CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT
5401 CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG
5461 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
5521 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
5581 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
5641 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
5701 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
5761 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
5821 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC
5881 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
5941 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
6001 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
6061 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
6121 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT
6181 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
6241 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
6301 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG
6361 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
6421 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
6481 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA
6541 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT
6601 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT
6661 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT
6721 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC
6781 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG
6841 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG
6901 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
6961 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC
7021 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG
7081 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG
7141 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT
7201 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT
7261 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG
7321 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA
7381 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC
7441 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGTGG AATGTGTGTC AGTTAGGGTG
7501 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
7561 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
7621 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC
7681 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC
7741 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT
7801 AGGCTTTTGC AAAAAGCTTG GACACAAGAC AGGCTTGCGA GATATGTTTG AGAATACCAC
7861 TTTATCCCGC GTCAGGGAGA GGCAGTGCGT AAAAAGACGC GGACTCATGT GAAATACTGG
7921 TTTTTAGTGC GCCAGATCTC TATAATCTCG CGCAACCTAT TTTCCCCTCG AACACTTTTT
7981 AAGCCGTAGA TAAACAGGCT GGGACACTTC ACATGAGCGA AAAATACATC GTCACCTGGG
8041 ACATGTTGCA GATCCATGCA CGTAAACTCG CAAGCCGACT GATGCCTTCT GAACAATGGA
8101 AAGGCATTAT TGCCGTAAGC CGTGGCGGTC TGTACCGGGT GCGTTACTGG CGCGTGAACT
8161 GGGTATTCGT CATGTCGATA CCGTTTGTAT TTCCAGCTAC GATCACGACA ACCAGCGCGA
8221 GCTTAAAGTG CTGAAACGCG CAGAAGGCGA TGGCGAAGGC TTCATCGTTA TTGATGACCT
8281 GGTGGATACC GGTGGTACTG CGGTTGCGAT TCGTGAAATG TATCCAAAAG CGCACTTTGT
8341 CACCATCTTC GCAAAACCGG CTGGTCGTCC GCTGGTTGAT GACTATGTTG TTGATATCCC
8401 GCAAGATACC TGGATTGAAC AGCCGTGGGA TATGGGCGTC GTATTCGTCC CGCCAATCTC
8461 CGGTCGCTAA TCTTTTCAAC GCCTGGCACT GCCGGGCGTT GTTCTTTTTA ACTTCAGGCG
8521 GGTTACAATA GTTTCCAGTA AGTATTCTGG AGGCTGCATC CATGACACAG GCAAACCTGA
8581 GCGAAACCCT GTTCAAACCC CGCTTTAAAC ATCCTGAAAC CTCGACGCTA GTCCGCCGCT
8641 TTAATCACGG CGCACAACCG CCTGTGCAGT CGGCCCTTGA TGGTAAAACC ATCCCTCACT
8701 GGTATCGCAT GATTAACCGT CTGATGTGGA TCTGGCGCGG CATTGACCCA CGCGAAATCC
8761 TCGACGTCCA GGCACGTATT GTGATGAGCG ATGCCGAACG TACCGACGAT GATTTATACG
8821 ATACGGTGAT TGGCTACCGT GGCGGCAACT GGATTTATGA GTGGGCCCCG GATCTTTGTG
8881 AAGGAACCTT ACTTCTGTGG TGTGACATAA TTGGACAAAC TACCTACAGA GATTTAAAGC
8941 TCTAAGGTAA ATATAAAATT TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT
9001 TGTGTATTTT AGATTCCAAC CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT
9061 AATGAGGAAA ACCTGTTTTG CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT
9121 GACTCTCAAC ATTCTACTCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT
9181 CCTTCAGAAT TGCTAAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC
9241 TTTGCTATTT ACACCACAAA GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA
9301 TATTCTGTAA CCTTTATAAG TAGGCATAAC AGTTATAATC ATAACATACT GTTTTTTCTT
9361 ACTCCACACA GGCATAGAGT GTCTGCTATT AATAACTATG CTCAAAAATT GTGTACCTTT
9421 AGCTTTTTAA TTTGTAAAGG GGTTAATAAG GAATATTTGA TGTATAGTGC CTTGACTAGA
9481 GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA
9541 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC
9601 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT
9661 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT
9721 CAACTGGATA ACTCAAGCTA ACCAAAATCA TCCCAAACTT CCCACCCCAT ACCCTATTAC
9781 CACTGCCAAT TACCTAGTGG TTTCATTTAC TCTAAACCTG TGATTCCTCT GAATTATTTT
9841 CATTTTAAAG AAATTGTATT TGTTAAATAT GTACTACAAA CTTAGT
//
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