北京华越洋生物
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pBBR1MCS

pBBR1MCS

pBBR1MCS



 

编号

载体名称

北京华越洋VECT75113

pBBR1MCS

 

pBBR1MCS载体基本信息:

载体名称:

pBBR1MCS, pBBRMCS1, pBBRMCS, pBBR1MCS-1, pBBRMCS-1

质粒类型:

广宿主蛋白表达或克隆载体质粒,广宿主载体质粒

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

--

载体大小:

4.7 kb

5' 测序引物及序列:

M13-F: TGTAAAACGACGGCCAGT

3' 测序引物及序列:

M13-R: CAGGAAACAGCTATGAC

载体标签:

--

载体抗性:

氯霉素

筛选标记:

--

克隆菌株:

DH5α

宿主细胞(系):

多种微生物物种,是广宿主质粒。

备注:

参考文献:
Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

GenBank sequenceXXU02374

稳定性/瞬表达:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pBBR1MCS载体质粒图谱和多克隆位点信息:

 

pBBR1MCS载体序列:

ORIGIN

    1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg

   61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt

  121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg

  181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg

  241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca

  301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt

  361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg

  421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt

  481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg

  541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct

  601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg

  661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc

  721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca

  781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg

  841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg

  901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc

  961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc

 1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga

 1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc

 1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt

 1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg

 1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac

 1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc

 1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg

 1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg

 1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag

 1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta

 1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg

 1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc

 1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac

 1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga

 1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct

 1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac

 1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt

 2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt

 2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag

 2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct

 2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga

 2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac

 2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct

 2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt

 2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg

 2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga

 2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga

 2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg

 2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa

 2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa

 2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag

 2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc

 2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca

 3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg

 3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg

 3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga

 3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc

 3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga

 3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct

 3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac

 3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac

 3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc

 3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata

 3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat

 3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg

 3721 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac

 3781 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg

 3841 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat

 3901 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt

 3961 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt

 4021 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct

 4081 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg

 4141 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat

 4201 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc

 4261 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac

 4321 ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat

 4381 caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt

 4441 cgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg

 4501 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc

 4561 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg

 4621 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca

 4681 gaacgggctt caggcgctcc cgaaggt

//

 

pBBR1MCS其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8