北京华越洋生物
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首页    载体及质粒    pBBR1MCS-2
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pBBR1MCS-2

pBBR1MCS-2

pBBR1MCS-2

 



编号

载体名称

北京华越洋VECT75114

pBBR1MCS-2

 

pBBR1MCS-2载体基本信息:

载体名称:

pBBR1MCS-2, pBBR1MCS2, pBBRMCS2

质粒类型:

广宿主蛋白表达或克隆载体质粒,广宿主载体质粒

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

--

载体大小:

5.1 kb

5' 测序引物及序列:

M13-F: TGTAAAACGACGGCCAGT

3' 测序引物及序列:

M13-R: CAGGAAACAGCTATGAC

载体标签:

--

载体抗性:

卡那霉素

筛选标记:

--

克隆菌株:

DH5α

宿主细胞(系):

多种微生物物种,是广宿主质粒。

备注:

参考文献:
[1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

[2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6.

GenBank sequenceU23751

稳定性/瞬表达:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pBBR1MCS-2载体质粒图谱和多克隆位点信息:

 

 

pBBR1MCS-2载体序列:

ORIGIN      

        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg

       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt

      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg

      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg

      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca

      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt

      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg

      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt

      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg

      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct

      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg

      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc

      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca

      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg

      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg

      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc

      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc

     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga

     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc

     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt

     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg

     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac

     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc

     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg

     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg

     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag

     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta

     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg

     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc

     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac

     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga

     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct

     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac

     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt

     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt

     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag

     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct

     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga

     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac

     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct

     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt

     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg

     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga

     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga

     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg

     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa

     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa

     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag

     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc

     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca

     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg

     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg

     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga

     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc

     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga

     3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct

     3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac

     3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac

     3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc

     3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata

     3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat

     3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg

     3721 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc

     3781 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt

     3841 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga

     3901 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa

     3961 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca

     4021 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa

     4081 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat

     4141 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct

     4201 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc

     4261 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca

     4321 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca

     4381 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa

     4441 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct

     4501 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc

     4561 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt

     4621 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt

     4681 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc

     4741 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag

     4801 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct

     4861 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg

     4921 tccagatagc ccagtagctg acattcatcc caggtggcac ttttcgggga aatgtgcgcg

     4981 cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct gttccgtcag

     5041 cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat cccgattcaa

     5101 cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggt

//

 

pBBR1MCS-2其他相关广宿主载体

 

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8