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pMHE7Tc

pMHE7Tc

pMHE7Tc

 



编号

载体名称

北京华越洋VECT75116

pMHE7Tc

 

pMHE7Tc载体基本信息:

载体名称:

pMHE7Tc

质粒类型:

广宿主载体;细菌表达载体;体外表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

6014 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

FLAG-tagStrep-tag II (N-ter)EK site

载体抗性:

四环素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE7Tc载体质粒图谱和多克隆位点信息:


pMHE7Tc载体序列:

ORIGIN      

        1 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga

       61 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac

      121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc

      181 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga

      241 aataattttg tttaacttta agaaggagat ataccatgga cgactacaag gacgacgacg

      301 acaagctcga gggcaactgg agccatccgc agttcgagaa gatcgaggga aggcctgtcg

      361 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccggc tgctaacaaa

      421 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt

      481 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg

      541 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg

      601 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc

      661 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct

      721 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt

      781 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt

      841 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca

      901 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc

      961 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc

     1021 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg

     1081 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa

     1141 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag

     1201 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt

     1261 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc

     1321 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag

     1381 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact

     1441 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac

     1501 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac

     1561 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt

     1621 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag

     1681 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc

     1741 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt

     1801 ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc

     1861 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca

     1921 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta

     1981 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct

     2041 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc

     2101 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc

     2161 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg

     2221 cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt

     2281 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc

     2341 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa

     2401 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg

     2461 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc

     2521 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc

     2581 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc

     2641 ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca

     2701 aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc

     2761 cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg

     2821 cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc

     2881 gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc

     2941 agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag

     3001 gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc

     3061 agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac

     3121 ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc

     3181 gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac

     3241 aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg

     3301 caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc

     3361 ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc

     3421 tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa

     3481 gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc

     3541 gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac

     3601 cttcgggagc gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag

     3661 gccaaggccg ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa

     3721 gtccagcgcc agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa

     3781 aagtgccacc tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat

     3841 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac

     3901 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag

     3961 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc

     4021 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct

     4081 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct

     4141 cactgatccg ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg

     4201 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt

     4261 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc

     4321 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa

     4381 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg

     4441 gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat

     4501 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg

     4561 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga

     4621 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt

     4681 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa

     4741 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga

     4801 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga

     4861 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca

     4921 tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga

     4981 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc

     5041 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt

     5101 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg

     5161 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg

     5221 tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc

     5281 ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat

     5341 atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca

     5401 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc

     5461 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat

     5521 ttaactgtga taaactaccg cattaaagct cgcgcagatc agttggaaga atttgtccac

     5581 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc

     5641 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct

     5701 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc

     5761 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg

     5821 cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccggtg

     5881 gatgaccttt tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg

     5941 tccccttttt tattttaaaa attttttcac aaaacggttt acaagcataa agctcaagga

     6001 gatggcgccc aaca

//

 

 

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