北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pMHE3
图片7

pMHE3

pMHE3

pMHE3



 

编号

载体名称

北京华越洋VECT75117

pMHE3

 

pMHE3载体基本信息:

载体名称:

pMHE3

质粒类型:

广宿主载体;细菌表达载体;体外表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

6290 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

T7 tag (N ter)6XHis tag (C ter)

载体抗性:

链霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE3载体质粒图谱和多克隆位点信息:


pMHE3载体序列:

ORIGIN      

        1 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc

       61 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact

      121 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt

      181 taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg tcgggatccg

      241 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga

      301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa

      361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga

      421 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg

      481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt

      541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc

      601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg

      661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga

      721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc

      781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa

      841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatattt tgttaaaatt

      901 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg

      961 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac

     1021 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg

     1081 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt

     1141 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc

     1201 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc

     1261 tgataaaaac ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc

     1321 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg

     1381 gctacagccg atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc

     1441 ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg

     1501 gctcatcggg catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca

     1561 aggctggcag gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg

     1621 ggccggtcgg tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat

     1681 gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg

     1741 acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg

     1801 acacggtgcc ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct

     1861 tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc

     1921 tgaccaccac ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg

     1981 ccgtgggttt cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca

     2041 cccagtgacc gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc

     2101 ttatctccat gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt

     2161 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct

     2221 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt

     2281 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc

     2341 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa

     2401 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg

     2461 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg

     2521 cttcgccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca

     2581 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca

     2641 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat

     2701 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag

     2761 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc

     2821 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg

     2881 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag

     2941 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag

     3001 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga

     3061 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa

     3121 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga

     3181 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac

     3241 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt

     3301 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga

     3361 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga

     3421 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt

     3481 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca

     3541 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg

     3601 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg

     3661 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc

     3721 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc

     3781 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg

     3841 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga

     3901 gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc

     3961 cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg

     4021 ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca

     4081 cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt

     4141 gaacgaattg ttaggtggcg gtacttgggt cgatagcttt atgcttgtaa accgttttgt

     4201 gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt agtaatataa

     4261 tctattaaag gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc cctcgctaga

     4321 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc

     4381 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa

     4441 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg

     4501 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga

     4561 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg

     4621 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct

     4681 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt

     4741 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc

     4801 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat

     4861 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat

     4921 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga

     4981 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat

     5041 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct

     5101 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg

     5161 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc

     5221 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct

     5281 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca

     5341 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata

     5401 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact

     5461 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc

     5521 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct

     5581 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc

     5641 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg

     5701 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg

     5761 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc

     5821 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag

     5881 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt

     5941 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca

     6001 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc

     6061 aggggaattg atccggtgga tgaccttttg aatgaccttt aatagattat attactaatt

     6121 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac

     6181 aagcataaag ctcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat

     6241 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt

//

 

pMHE3其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8