北京华越洋生物
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pBTB-3

pBTB-3

pBTB-3



 

编号

载体名称

北京华越洋VECT75121

pBTB-3

 

pBTB-3载体基本信息:

载体名称:

pBTB-3

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

3586 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氯霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBTB-3载体质粒图谱和多克隆位点信息:

pBTB-3载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca

     1681 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt

     1741 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc

     1801 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat

     1861 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga

     1921 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct

     1981 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg

     2041 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga

     2101 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta

     2161 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga

     2221 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg

     2281 cggggcgtaa acgcgtggat ccccctcaag tcaaaagcct ccggtcggag gcttttgact

     2341 ttctgctatg gaggtcaggt atgattttta tgacaacttg acggctacat cattcacttt

     2401 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg

     2461 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg

     2521 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac

     2581 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg

     2641 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca

     2701 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg

     2761 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc

     2821 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc

     2881 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta

     2941 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc

     3001 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc

     3061 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt

     3121 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa

     3181 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc

     3241 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag

     3301 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct

     3361 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag

     3421 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta

     3481 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact

     3541 ctctactgtt tctccatacc cgtttttttg ggctagagaa ttcgtc

//

 

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