FUW-M2rtTA
FUW-M2rtTA
编号 | 载体名称 |
北京华越洋VECT231287 | FUW-M2rtTA |
FUW-M2rtTA载体基本信息：
载体名称: | FUW-M2rtTA |
质粒类型: | 慢病毒载体；四环素调控载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | Ubc |
载体大小: | 7979 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 大部分哺乳动物细胞 |
备注: | FUW-M2rtTA vector expressing the reverse tetracycline transactivator |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
FUW-M2rtTA载体质粒图谱和多克隆位点信息：
FUW-M2rtTA载体简介：
Lentiviral plasmid expressing the reverse tetracycline transactivator.
FUW-M2rtTA载体序列：
ORIGIN
1 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
61 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
121 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA
181 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC
241 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT
301 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG
361 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT
421 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC
481 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG
541 TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA
601 CCGTCGACCT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC
661 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC
721 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT
781 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA
841 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC
901 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA
961 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA
1021 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT
1081 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT
1141 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
1201 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
1261 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGG GCCCGTTTAA ACCCGCTGAT
1321 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
1381 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
1441 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
1501 GGGAGGATTG GGAAGACAAT AGCAGGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
1561 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
1621 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
1681 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
1741 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
1801 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
1861 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
1921 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
1981 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
2041 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
2101 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
2161 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
2221 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
2281 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
2341 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
2401 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
2461 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
2521 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
2581 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
2641 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
2701 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
2761 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
2821 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
2881 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
2941 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
3001 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
3061 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
3121 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
3181 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
3241 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
3301 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC
3361 CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT
3421 ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA
3481 CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGGGTT AGGCGTTTTG
3541 CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA TTGATTATTG ACTAGTTATT
3601 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT
3661 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
3721 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG
3781 AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
3841 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT
3901 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA
3961 TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA
4021 GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC
4081 CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG
4141 AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA
4201 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT
4261 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC
4321 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG
4381 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG
4441 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA
4501 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG
4561 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG
4621 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT
4681 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT
4741 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA
4801 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC
4861 AAGCGGCCGC TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG GAGAAGTGAA
4921 TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG
4981 AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTC
5041 TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA
5101 CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGAGGCGCAA
5161 CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG AATCCTGGCT
5221 GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC TGGAAAACTC
5281 ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT GGAACAGATT
5341 TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTAATA
5401 CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA ATTATTGGAA
5461 TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT GTGGTATATA
5521 AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT TGCTGTACTT
5581 TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC CCACCTCCCA
5641 ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA GAGAGACAGA
5701 GACAGATCCA TTCGATTAGT GAACGGATCG GCACTGCGTG CGCCAATTCT GCAGACAAAT
5761 GGCAGTATTC ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA
5821 AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC
5881 AAAAATTCAA AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT GGTTAATTAA
5941 CCCGTGTCGG CTCCAGATCT GGCCTCCGCG CCGGGTTTTG GCGCCTCCCG CGGGCGCCCC
6001 CCTCCTCACG GCGAGCGCTG CCACGTCAGA CGAAGGGCGC AGCGAGCGTC CTGATCCTTC
6061 CGCCCGGACG CTCAGGACAG CGGCCCGCTG CTCATAAGAC TCGGCCTTAG AACCCCAGTA
6121 TCAGCAGAAG GACATTTTAG GACGGGACTT GGGTGACTCT AGGGCACTGG TTTTCTTTCC
6181 AGAGAGCGGA ACAGGCGAGG AAAAGTAGTC CCTTCTCGGC GATTCTGCGG AGGGATCTCC
6241 GTGGGGCGGT GAACGCCGAT GATTATATAA GGACGCGCCG GGTGTGGCAC AGCTAGTTCC
6301 GTCGCAGCCG GGATTTGGGT CGCGGTTCTT GTTTGTGGAT CGCTGTGATC GTCACTTGGT
6361 GAGTAGCGGG CTGCTGGGCT GGCCGGGGCT TTCGTGGCCG CCGGGCCGCT CGGTGGGACG
6421 GAAGCGTGTG GAGAGACCGC CAAGGGCTGT AGTCTGGGTC CGCGAGCAAG GTTGCCCTGA
6481 ACTGGGGGTT GGGGGGAGCG CAGCAAAATG GCGGCTGTTC CCGAGTCTTG AATGGAAGAC
6541 GCTTGTGAGG CGGGCTGTGA GGTCGTTGAA ACAAGGTGGG GGGCATGGTG GGCGGCAAGA
6601 ACCCAAGGTC TTGAGGCCTT CGCTAATGCG GGAAAGCTCT TATTCGGGTG AGATGGGCTG
6661 GGGCACCATC TGGGGACCCT GACGTGAAGT TTGTCACTGA CTGGAGAACT CGGTTTGTCG
6721 TCTGTTGCGG GGGCGGCAGT TATGGCGGTG CCGTTGGGCA GTGCACCCGT ACCTTTGGGA
6781 GCGCGCGCCC TCGTCGTGTC GTGACGTCAC CCGTTCTGTT GGCTTATAAT GCAGGGTGGG
6841 GCCACCTGCC GGTAGGTGTG CGGTAGGCTT TTCTCCGTCG CAGGACGCAG GGTTCGGGCC
6901 TAGGGTAGGC TCTCCTGAAT CGACAGGCGC CGGACCTCTG GTGAGGGGAG GGATAAGTGA
6961 GGCGTCAGTT TCTTTGGTCG GTTTTATGTA CCTATCTTCT TAAGTAGCTG AAGCTCCGGT
7021 TTTGAACTAT GCGCTCGGGG TTGGCGAGTG TGTTTTGTGA AGTTTTTTAG GCACCTTTTG
7081 AAATGTAATC ATTTGGGTCA ATATGTAATT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA
7141 AATTCTGGCC GTTTTTGGCT TTTTTGTTAG ACGAAGCTTG GGCCCGGGAA TTAATTCACC
7201 ATGTCTAGAC TGGACAAGAG CAAAGTCATA AACGGCGCTC TGGAATTACT CAATGGAGTC
7261 GGTATCGAAG GCCTGACGAC AAGGAAACTC GCTCAAAAGC TGGGAGTTGA GCAGCCTACC
7321 CTGTACTGGC ACGTGAAGAA CAAGCGGGCC CTGCTCGATG CCCTGCCAAT CGAGATGCTG
7381 GACAGGCATC ATACCCACTT CTGCCCCCTG GAAGGCGAGT CATGGCAAGA CTTTCTGCGG
7441 AACAACGCCA AGTCATTCCG CTGTGCTCTC CTCTCACATC GCGACGGGGC TAAAGTGCAT
7501 CTCGGCACCC GCCCAACAGA GAAACAGTAC GAAACCCTGG AAAATCAGCT CGCGTTCCTG
7561 TGTCAGCAAG GCTTCTCCCT GGAGAACGCA CTGTACGCTC TGTCCGCCGT GGGCCACTTT
7621 ACACTGGGCT GCGTATTGGA GGAACAGGAG CATCAAGTAG CAAAAGAGGA AAGAGAGACA
7681 CCTACCACCG ATTCTATGCC CCCACTTCTG AGACAAGCAA TTGAGCTGTT CGACCGGCAG
7741 GGAGCCGAAC CTGCCTTCCT TTTCGGCCTG GAACTAATCA TATGTGGCCT GGAGAAACAG
7801 CTAAAGTGCG AAAGCGGCGG GCCGGCCGAC GCCCTTGACG ATTTTGACTT AGACATGCTC
7861 CCAGCCGATG CCCTTGACGA CTTTGACCTT GATATGCTGC CTGCTGACGC TCTTGACGAT
7921 TTTGACCTTG ACATGCTCCC CGGGTAACTA AGTAAGGATC AATTCGATAT CAAGCTTAT
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FUW-M2rtTA其他相关慢病毒载体：
