北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pLOX-TERT-iresTK
1

pLOX-TERT-iresTK

pLOX-TERT-iresTK

pLOX-TERT-iresTK


 

编号

载体名称

北京华越洋VECT231301

pLOX-TERT-iresTK

 

pLOX-TERT-iresTK载体基本信息:

载体名称:

pLOX-TERT-iresTK

质粒类型:

慢病毒系统载体

高拷贝/低拷贝:

高拷贝

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

13117bp

5' 测序引物及序列:

CMV   fwd: CGCAAATGGGCGGTAGGCGTG

3' 测序引物及序列:

SP6:   ATTTAGGTGACACTATAG

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillian

筛选标记:

博来霉素(Zeocin

备注:

克隆菌株为Stbl3HB101

稳定性:

--

组成型:

--

病毒/非病毒:

慢病毒

 

pLOX-TERT-iresTK载体质粒图谱和多克隆位点信息:

 

pLOX-TERT-iresTK载体简介:

CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK.

During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites

end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.

 

pLOX-TERT-iresTK载体序列:

ORIGIN

    1 AGTTGGAAGG GCTAATTCAC TCCCAAAGAA GACAAGATAT CCTTGATCTG TGGATCTACC

   61 ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC

  121 CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG

  181 CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC

  241 CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC

  301 GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT

  361 CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA

  421 GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC

  481 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG

  541 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC

  601 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA

  661 AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA

  721 CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT

  781 AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT

  841 GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT

  901 GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG

  961 GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA

 1021 GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG

 1081 ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC

 1141 AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT

 1201 GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA

 1261 AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG

 1321 TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC

 1381 AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG

 1441 CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG

 1501 GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA

 1561 CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG

 1621 ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA

 1681 ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG

 1741 GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT

 1801 ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA

 1861 CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC

 1921 CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC

 1981 AGAGACAGAT CCATTCGATT AGTGAACGGA TCTCGACGGT ATCGATAAGC TTGGGAGTTC

 2041 CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA

 2101 TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT

 2161 CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG

 2221 CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG

 2281 TACATGACCT TACGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT

 2341 ACCATGGTGA TGCGGTTTTG GCAGTACACC AATGGGCGTG GATAGCGGTT TGACTCACGG

 2401 GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA

 2461 CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT

 2521 GTACGGTGGG AGGTCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA

 2581 CGCCATCCAC GCTGTTTTGA CCTCCATAGA AGACACCGAC TCTAGCTAGA GGATCTACTA

 2641 GTGAATTCCT GCTGCGCACG TGGGAAGCCC TGGCCCCGGC CACCCCCGCG ATGCCGCGCG

 2701 CTCCCCGCTG CCGAGCCGTG CGCTCCCTGC TGCGCAGCCA CTACCGCGAG GTGCTGCCGC

 2761 TGGCCACGTT CGTGCGGCGC CTGGGGCCCC AGGGCTGGCG GCTGGTGCAG CGCGGGGACC

 2821 CGGCGGCTTT CCGCGCGCTG GTGGCCCAGT GCCTGGTGTG CGTGCCCTGG GACGCACGGC

 2881 CGCCCCCCGC CGCCCCCTCC TTCCGCCAGG TGTCCTGCCT GAAGGAGCTG GTGGCCCGAG

 2941 TGCTGCAGAG GCTGTGCGAG CGCGGCGCGA AGAACGTGCT GGCCTTCGGC TTCGCGCTGC

 3001 TGGACGGGGC CCGCGGGGGC CCCCCCGAGG CCTTCACCAC CAGCGTGCGC AGCTACCTGC

 3061 CCAACACGGT GACCGACGCA CTGCGGGGGA GCGGGGCGTG GGGGCTGCTG TTGCGCCGCG

 3121 TGGGCGACGA CGTGCTGGTT CACCTGCTGG CACGCTGCGC GCTCTTTGTG CTGGTGGCTC

 3181 CCAGCTGCGC CTACCAGGTG TGCGGGCCGC CGCTGTACCA GCTCGGCGCT GCCACTCAGG

 3241 CCCGGCCCCC GCCACACGCT AGTGGACCCC GAAGGCGTCT GGGATGCGAA CGGGCCTGGA

 3301 ACCATAGCGT CAGGGAGGCC GGGGTCCCCC TGGGCCTGCC AGCCCCGGGT GCGAGGAGGC

 3361 GCGGGGGCAG TGCCAGCCGA AGTCTGCCGT TGCCCAAGAG GCCCAGGCGT GGCGCTGCCC

 3421 CTGAGCCGGA GCGGACGCCC GTTGGGCAGG GGTCCTGGGC CCACCCGGGC AGGACGCGTG

 3481 GACCGAGTGA CCGTGGTTTC TGTGTGGTGT CACCTGCCAG ACCCGCCGAA GAAGCCACCT

 3541 CTTTGGAGGG TGCGCTCTCT GGCACGCGCC ACTCCCACCC ATCCGTGGGC CGCCAGCACC

 3601 ACGCGGGCCC CCCATCCACA TCGCGGCCAC CACGTCCCTG GGACACGCCT TGTCCCCCGG

 3661 TGTACGCCGA GACCAAGCAC TTCCTCTACT CCTCAGGCGA CAAGGAGCAG CTGCGGCCCT

 3721 CCTTCCTACT CAGCTCTCTG AGGCCCAGCC TGACTGGCGC TCGGAGGCTC GTGGAGACCA

 3781 TCTTTCTGGG TTCCAGGCCC TGGATGCCAG GGACTCCCCG CAGGTTGCCC CGCCTGCCCC

 3841 AGCGCTACTG GCAAATGCGG CCCCTGTTTC TGGAGCTGCT TGGGAACCAC GCGCAGTGCC

 3901 CCTACGGGGT GCTCCTCAAG ACGCACTGCC CGCTGCGAGC TGCGGTCACC CCAGCAGCCG

 3961 GTGTCTGTGC CCGGGAGAAG CCCCAGGGCT CTGTGGCGGC CCCCGAGGAG GAGGACACAG

 4021 ACCCCCGTCG CCTGGTGCAG CTGCTCCGCC AGCACAGCAG CCCCTGGCAG GTGTACGGCT

 4081 TCGTGCGGGC CTGCCTGCGC CGGCTGGTGC CCCCAGGCCT CTGGGGCTCC AGGCACAACG

 4141 AACGCCGCTT CCTCAGGAAC ACCAAGAAGT TCATCTCCCT GGGGAAGCAT GCCAAGCTCT

 4201 CGCTGCAGGA GCTGACGTGG AAGATGAGCG TGCGGGGCTG CGCTTGGCTG CGCAGGAGCC

 4261 CAGGGGTTGG CTGTGTTCCG GCCGCAGAGC ACCGTCTGCG TGAGGAGATC CTGGCCAAGT

 4321 TCCTGCACTG GCTGATGAGT GTGTACGTCG TCGAGCTGCT CAGGTCTTTC TTTTATGTCA

 4381 CGGAGACCAC GTTTCAAAAG AACAGGCTCT TTTTCTACCG GAAGAGTGTC TGGAGCAAGT

 4441 TGCAAAGCAT TGGAATCAGA CAGCACTTGA AGAGGGTGCA GCTGCGGGAG CTGTCGGAAG

 4501 CAGAGGTCAG GCAGCATCGG GAAGCCAGGC CCGCCCTGCT GACGTCCAGA CTCCGCTTCA

 4561 TCCCCAAGCC TGACGGGCTG CGGCCGATTG TGAACATGGA CTACGTCGTG GGAGCCAGAA

 4621 CGTTCCGCAG AGAAAAGAGG GCCGAGCGTC TCACCTCGAG GGTGAAGGCA CTGTTCAGCG

 4681 TGCTCAACTA CGAGCGGGCG CGGCGCCCCG GCCTCCTGGG CGCCTCTGTG CTGGGCCTGG

 4741 ACGATATCCA CAGGGCCTGG CGCACCTTCG TGCTGCGTGT GCGGGCCCAG GACCCGCCGC

 4801 CTGAGCTGTA CTTTGTCAAG GTGGATGTGA CGGGCGCGTA CGACACCATC CCCCAGGACA

 4861 GGCTCACGGA GGTCATCGCC AGCATCATCA AACCCCAGAA CACGTACTGC GTGCGTCGGT

 4921 ATGCCGTGGT CCAGAAGGCC GCCCATGGGC ACGTCCGCAA GGCCTTCAAG AGCCACGTCT

 4981 CTACCTTGAC AGACCTCCAG CCGTACATGC GACAGTTCGT GGCTCACCTG CAGGAGACCA

 5041 GCCCGCTGAG GGATGCCGTC GTCATCGAGC AGAGCTCCTC CCTGAATGAG GCCAGCAGTG

 5101 GCCTCTTCGA CGTCTTCCTA CGCTTCATGT GCCACCACGC CGTGCGCATC AGGGGCAAGT

 5161 CCTACGTCCA GTGCCAGGGG ATCCCGCAGG GCTCCATCCT CTCCACGCTG CTCTGCAGCC

 5221 TGTGCTACGG CGACATGGAG AACAAGCTGT TTGCGGGGAT TCGGCGGGAC GGGCTGCTCC

 5281 TGCGTTTGGT GGATGATTTC TTGTTGGTGA CACCTCACCT CACCCACGCG AAAACCTTCC

 5341 TCAGGACCCT GGTCCGAGGT GTCCCTGAGT ATGGCTGCGT GGTGAACTTG CGGAAGACAG

 5401 TGGTGAACTT CCCTGTAGAA GACGAGGCCC TGGGTGGCAC GGCTTTTGTT CAGATGCCGG

 5461 CCCACGGCCT ATTCCCCTGG TGCGGCCTGC TGCTGGATAC CCGGACCCTG GAGGTGCAGA

 5521 GCGACTACTC CAGCTATGCC CGGACCTCCA TCAGAGCCAG TCTCACCTTC AACCGCGGCT

 5581 TCAAGGCTGG GAGGAACATG CGTCGCAAAC TCTTTGGGGT CTTGCGGCTG AAGTGTCACA

 5641 GCCTGTTTCT GGATTTGCAG GTGAACAGCC TCCAGACGGT GTGCACCAAC ATCTACAAGA

 5701 TCCTCCTGCT GCAGGCGTAC AGGTTTCACG CATGTGTGCT GCAGCTCCCA TTTCATCAGC

 5761 AAGTTTGGAA GAACCCCACA TTTTTCCTGC GCGTCATCTC TGACACGGCC TCCCTCTGCT

 5821 ACTCCATCCT GAAAGCCAAG AACGCAGGGA TGTCGCTGGG GGCCAAGGGC GCCGCCGGCC

 5881 CTCTGCCCTC CGAGGCCGTG CAGTGGCTGT GCCACCAAGC ATTCCTGCTC AAGCTGACTC

 5941 GACACCGTGT CACCTACGTG CCACTCCTGG GGTCACTCAG GACAGCCCAG ACGCAGCTGA

 6001 GTCGGAAGCT CCCGGGGACG ACGCTGACTG CCCTGGAGGC CGCAGCCAAC CCGGCACTGC

 6061 CCTCAGACTT CAAGACCATC CTGGACTGAT GGCGTCGACA TTAATTCCGG TTATTTTCCA

 6121 CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA

 6181 GCATTCCTAG GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA

 6241 AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA

 6301 GGCAGCGGAA CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG

 6361 ATACACCTGC AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA

 6421 GAGTCAAATG GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC

 6481 CCCATTGTAT GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA

 6541 GGTTAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG

 6601 ATGATAATAC CATGGCTTCG TACCCCTGCC ATCAACACGC GTCTGCGTTC GACCAGGCTG

 6661 CGCGTTCTCG CGGCCATAGC AACCGACGTA CGGCGTTGCG CCCTCGCCGG CAGCAAGAAG

 6721 CCACGGAAGT CCGCCTGGAG CAGAAAATGC CCACGCTACT GCGGGTTTAT ATAGACGGTC

 6781 CTCACGGGAT GGGGAAAACC ACCACCACGC AACTGCTGGT GGCCCTGGGT TCGCGCGACG

 6841 ATATCGTCTA CGTACCCGAG CCGATGACTT ACTGGCAGGT GCTGGGGGCT TCCGAGACAA

 6901 TCGCGAACAT CTACACCACA CAACACCGCC TCGACCAGGG TGAGATATCG GCCGGGGACG

 6961 CGGCGGTGGT AATGACAAGC GCCCAGATAA CAATGGGCAT GCCTTATGCC GTGACCGACG

 7021 CCGTTCTGGC TCCTCATATC GGGGGGGAGG CTGGGAGCTC ACATGCCCCG CCCCCGGCCC

 7081 TCACCCTCAT CTTCGACCGC CATCCCATCG CCGCCCTCCT GTGCTACCCG GCCGCGCGAT

 7141 ACCTTATGGG CAGCATGACC CCCCAGGCCG TGCTGGCGTT CGTGGCCCTC ATCCCGCCGA

 7201 CCTTGCCCGG CACAAACATC GTGTTGGGGG CCCTTCCGGA GGACAGACAC ATCGACCGCC

 7261 TGGCCAAACG CCAGCGCCCC GGCGAGCGGC TTGACCTGGC TATGCTGGCC GCGATTCGCC

 7321 GCGTTTACGG GCTGCTTGCC AATACGGTGC GGTATCTGCA GGGCGGCGGG TCGTGGCGGG

 7381 AGGATTGGGG ACAGCTTTCG GGGACGGCCG TGCCGCCCCA GGGTGCCGAG CCCCAGAGCA

 7441 ACGCGGGCCC ACGACCCCAT ATCGGGGACA CGTTATTTAC CCTGTTTCGG GCCCCCGAGT

 7501 TGCTGGCCCC CAACGGCGAC CTGTATAACG TGTTTGCCTG GGCTTTGGCT CGACGGTACC

 7561 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG

 7621 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT GGGATCAATT CACCATGGGA

 7681 ATAACTTCGT ATAGCATACA TTATACGAAG TTATGCTGCT TTTTGCTTGT ACTGGGTCTC

 7741 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA

 7801 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT

 7861 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATCT

 7921 AGAATTAATT CCGTGTATTC TATAGTGTCA CCTAAATCGT ATGTGTATGA TACATAAGGT

 7981 TATGTATTAA TTGTAGCCGC GTTCTAACGA CAATATGTAC AAGCCTAATT GTGTAGCATC

 8041 TGGCTTACTG AAGCAGACCC TATCATCTCT CTCGTAAACT GCCGTCAGAG TCGGTTTGGT

 8101 TGGACGAACC TTCTGAGTTT CTGGTAACGC CGTCCCGCAC CCGGAAATGG TCAGCGAACC

 8161 AATCAGCAGG GTCATCGCTA GCCAGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC

 8221 CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG

 8281 GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT

 8341 GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT

 8401 CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG

 8461 TCGAATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC

 8521 ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA

 8581 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA

 8641 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA

 8701 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT

 8761 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA

 8821 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA

 8881 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG

 8941 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA

 9001 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA

 9061 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC

 9121 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC

 9181 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA

 9241 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC

 9301 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA

 9361 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC

 9421 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG

 9481 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG

 9541 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG

 9601 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC

 9661 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC

 9721 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT

 9781 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC

 9841 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC

 9901 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG

 9961 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA

10021 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC

10081 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT

10141 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA

10201 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC

10261 TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC

10321 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT

10381 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT

10441 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC

10501 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG

10561 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC

10621 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG

10681 CGCGTTGGCC GATTCATTAA TGCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC

10741 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG

10801 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA

10861 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC

10921 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC

10981 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG

11041 CAAAAAGCTT GGACACAAGA CAGGCTTGCG AGATATGTTT GAGAATACCA CTTTATCCCG

11101 CGTCAGGGAG AGGCAGTGCG TAAAAAGACG CGGACTCATG TGAAATACTG GTTTTTAGTG

11161 CGCCAGATCT CTATAATCTC GCGCAACCTA TTTTCCCCTC GAACACTTTT TAAGCCGTAG

11221 ATAAACAGGC TGGGACACTT CACATGAGCG AAAAATACAT CGTCACCTGG GACATGTTGC

11281 AGATCCATGC ACGTAAACTC GCAAGCCGAC TGATGCCTTC TGAACAATGG AAAGGCATTA

11341 TTGCCGTAAG CCGTGGCGGT CTGTACCGGG TGCGTTACTG GCGCGTGAAC TGGGTATTCG

11401 TCATGTCGAT ACCGTTTGTA TTTCCAGCTA CGATCACGAC AACCAGCGCG AGCTTAAAGT

11461 GCTGAAACGC GCAGAAGGCG ATGGCGAAGG CTTCATCGTT ATTGATGACC TGGTGGATAC

11521 CGGTGGTACT GCGGTTGCGA TTCGTGAAAT GTATCCAAAA GCGCACTTTG TCACCATCTT

11581 CGCAAAACCG GCTGGTCGTC CGCTGGTTGA TGACTATGTT GTTGATATCC CGCAAGATAC

11641 CTGGATTGAA CAGCCGTGGG ATATGGGCGT CGTATTCGTC CCGCCAATCT CCGGTCGCTA

11701 ATCTTTTCAA CGCCTGGCAC TGCCGGGCGT TGTTCTTTTT AACTTCAGGC GGGTTACAAT

11761 AGTTTCCAGT AAGTATTCTG GAGGCTGCAT CCATGACACA GGCAAACCTG AGCGAAACCC

11821 TGTTCAAACC CCGCTTTAAA CATCCTGAAA CCTCGACGCT AGTCCGCCGC TTTAATCACG

11881 GCGCACAACC GCCTGTGCAG TCGGCCCTTG ATGGTAAAAC CATCCCTCAC TGGTATCGCA

11941 TGATTAACCG TCTGATGTGG ATCTGGCGCG GCATTGACCC ACGCGAAATC CTCGACGTCC

12001 AGGCACGTAT TGTGATGAGC GATGCCGAAC GTACCGACGA TGATTTATAC GATACGGTGA

12061 TTGGCTACCG TGGCGGCAAC TGGATTTATG AGTGGGCCCC GGATCTTTGT GAAGGAACCT

12121 TACTTCTGTG GTGTGACATA ATTGGACAAA CTACCTACAG AGATTTAAAG CTCTAAGGTA

12181 AATATAAAAT TTTTAAGTGT ATAATGTGTT AAACTACTGA TTCTAATTGT TTGTGTATTT

12241 TAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT TAATGAGGAA

12301 AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC TGACTCTCAA

12361 CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT TCCTTCAGAA

12421 TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG CTTTGCTATT

12481 TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA ATATTCTGTA

12541 ACCTTTATAA GTAGGCATAA CAGTTATAAT CATAACATAC TGTTTTTTCT TACTCCACAC

12601 AGGCATAGAG TGTCTGCTAT TAATAACTAT GCTCAAAAAT TGTGTACCTT TAGCTTTTTA

12661 ATTTGTAAAG GGGTTAATAA GGAATATTTG ATGTATAGTG CCTTGACTAG AGATCATAAT

12721 CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT

12781 GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA

12841 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA

12901 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCAACTGGAT

12961 AACTCAAGCT AACCAAAATC ATCCCAAACT TCCCACCCCA TACCCTATTA CCACTGCCAA

13021 TTACCTAGTG GTTTCATTTA CTCTAAACCT GTGATTCCTC TGAATTATTT TCATTTTAAA

13081 GAAATTGTAT TTGTTAAATA TGTACTACAA ACTTAGT

//


pLOX-TERT-iresTK其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-MetLuc   Control

pLVX-EF1α-AcGFP1-N1

pLVX-Het-2

pLVX-IRES-Neo

pSIH1-H1-CopGFP

pLentilox   3.7

pRSV-rev

pLVX-DD-AmCyan1   Reporter

pLVX-Het-1

pMDLg-pRRE

pLVX-PTuner

pLVX-PTuner-Green

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-DD-tdTomato   Control

pLVX-TetOne

pLVX-DD-AmCyan1    Control

pLVX-rHom-Nuc1

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-mCherry

pLVX-CherryPicker   Control

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

FUGW

pLenti6.3-EmGFP-BveI   miR

pGIPZ

pLenti6.3-MCS

pLenti6.3-BveI     miR

pSicoR

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

VSV-G

pLentG-KOSM

pMDLg/pRRE

pFUGW

pLVX-TRE3G-Luc     Control

pLP2

pSico

pLenti6.3-MCS-IRES2-DsRed2

pGensil-1

pcDNA6.2-EmGFP-NC-   I

FUW-tetO-hOCT4

pSico   PGK Puro

FUW

pLVX-shRNA2

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLKO.1-TRC     control

pPACKH1-GAG

pMD2.G

pLKO.1-TRC

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLKO.1-hygro

FUW-tetO-hKLF4

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1    Control

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pLVX-Hom-1

pLVX-shRNA1

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI   miR

pLVX-CherryPicker1

pLVX-TRE3G

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1

pLVX-IRES-mCherry

pLP1

pLVX-tdTomato-C1

pLOX-Ttag-iresTK

pLVX-Tet-On-Advanced

pCMV-dR8.91

pLVX-IRES-Hyg

pCgpv

pLVX-EF1α-AcGFP1-C1

pLVTHM

pLVX-EF1α-IRES-ZsGreen1

pPACKH1-REV

pLVX-Hom-Mem1

pLKO.1-puro-GFP-siRNA

pCDH-CMV-MCS-EF1-Puro

FUW-tetO-hMYC

pPRIME-TET-GFP-FF3

FUW-M2rtTA

pLOX-CW-CRE

pLVX-AmCyan1-C1

pLL3.7

pLVX-DsRed-Monomer-C1

pLVX-DD-tdTomato     Reporter

pLVX-ZsGreen1-C1

pLVX-CherryPicker2

pLVX-IRES-tdTomato

pLVX-TetOne-Puro

pLVX-tdTomato-N1

pLVX-rHom-Sec1

pLVX-Tight-Puro

pLVX-Het-Nuc1

pLVX-EF1α-mCherry-N1

pLVX-DD-ZsGreen1     Reporter

pLVX-EF1α-IRES-Puro

pCDH-CMV-MCS-EF1-Neo

pLVX-Het-Mem1

pCDF1-MCS2-EF1-Puro

pLP/VSVG

pLVX-TRE3G-ZsGreen1

pcDNA6.2-EmGFP-MCS1   miR

pLenti6/V5-GW/lacZ

pcDNA6.2-BsaI   miR

pLenti6.3-DsRed2-BveI   miR

pTRIPZ

psPAX2

LeGO-iC2

pcDNA6.2-DsRed2-MCS1   miR

pLKO.3G

pcDNA6.2-EmGFP-BsaI   miR

pLVX-Puro

pLEX-MCS

pSicoR   PGK Puro