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pMMB207

pMMB207

pMMB207




编号

载体名称

北京华越洋VECT75125

pMMB207

 

pMMB207载体基本信息:

载体名称:

pMMB207

质粒类型:

细菌表达载体;广宿主载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

Tac

载体大小:

9075 bp

5' 测序引物及序列

Tac promoterGAGCGGATAACAATTTCACACAGG

3' 测序引物及序列:

pBAD Reverse: GATTTAATCTGTATCAGG

载体标签:

--

载体抗性:

氨苄青霉素和氯霉素(10 mg/ml Chloramphenicol

表达菌株:

原核细胞

备注:

--

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pMMB207载体质粒图谱和多克隆位点信息:

pMMB207载体序列:

ORIGIN

    1 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc

   61 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc

  121 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa

  181 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg

  241 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg

  301 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag

  361 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat

  421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg

  481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc

  541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga

  601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga

  661 gagttttcgc cccgaagaac gtgatgtccg gcggtgcttt tgccgttacg caccaccccg

  721 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc aagcacctca

  781 aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg

  841 aataaatacc tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg

  901 ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag

  961 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga

 1021 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg

 1081 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta

 1141 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata

 1201 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg

 1261 aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt

 1321 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg

 1381 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg

 1441 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga

 1501 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca

 1561 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc

 1621 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact

 1681 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattgtctt caaattcccg

 1741 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta

 1801 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc

 1861 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc

 1921 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa

 1981 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga

 2041 acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg

 2101 tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc

 2161 catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc

 2221 tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta

 2281 ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc

 2341 ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc

 2401 cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc

 2461 agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt

 2521 caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct

 2581 tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct

 2641 gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga

 2701 gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt

 2761 aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg

 2821 taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg

 2881 gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag

 2941 catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag

 3001 ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt

 3061 gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc

 3121 tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc

 3181 ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac

 3241 cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca

 3301 ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg

 3361 gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct

 3421 caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc

 3481 attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat

 3541 cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat

 3601 ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga

 3661 cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca

 3721 gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg

 3781 ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg

 3841 agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc

 3901 gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt

 3961 ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc

 4021 tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct

 4081 ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct

 4141 caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat

 4201 gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat

 4261 gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt

 4321 gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc

 4381 tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc

 4441 cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga

 4501 cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga

 4561 caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa

 4621 gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca

 4681 gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag

 4741 ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg

 4801 cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg

 4861 ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg

 4921 caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc

 4981 cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat

 5041 ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata

 5101 ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc

 5161 ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc

 5221 aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg

 5281 ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga

 5341 ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg

 5401 tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct

 5461 ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc

 5521 agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc

 5581 aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct

 5641 ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg

 5701 tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct

 5761 gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc

 5821 caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca

 5881 agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc

 5941 cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga

 6001 cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat

 6061 cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc

 6121 cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc

 6181 ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc

 6241 cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa

 6301 ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct

 6361 caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta

 6421 agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc

 6481 tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact

 6541 acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc

 6601 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac

 6661 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca

 6721 ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag

 6781 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg

 6841 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg

 6901 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg

 6961 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg

 7021 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca

 7081 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca

 7141 tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga

 7201 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac

 7261 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc

 7321 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa

 7381 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca

 7441 gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt

 7501 gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa

 7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta

 7621 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc

 7681 accgcctggc cctgagagag ttgcagcaag cggtccacgt ggtttgcccc agcaggcgaa

 7741 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt

 7801 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg

 7861 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca

 7921 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta

 7981 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg

 8041 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat

 8101 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct

 8161 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg

 8221 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat

 8281 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc

 8341 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca

 8401 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg

 8461 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt

 8521 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg

 8581 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct

 8641 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt

 8701 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg

 8761 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg

 8821 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg

 8881 cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc

 8941 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg

 9001 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggctgttttg

 9061 gcggatgaga gaaga

//

 

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pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

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pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

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pMMB207

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pCS2+

pCS2-

pCS111

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pCS107

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