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首页    载体及质粒    pCS111
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pCS111

pCS111

pCS111

 



编号

载体名称

北京华越洋VECT75126

pCS111

 

pCS111载体基本信息:

载体名称:

pCS111

质粒类型:

克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

simian CMV IE94lacT3SP6

载体大小:

4146 bp

5' 测序引物及序列:

SP6 fwdMCS1)、T3 fwdMCS2

3' 测序引物及序列:

EBV revMCS1)、SV40 polyA revMCS2

载体标签:

载体抗性:

氨苄青霉素

克隆菌株:

DH10B

表达细胞:

原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。

备注:

pCS111载体能够在多种细胞中进行高水平的瞬表达。pCS111载体同时也能进行体外转录和翻译。

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pCS111载体质粒图谱和多克隆位点信息:


pCS111载体序列:

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcccgggaat ctggccgcgg

      121 ccgcaaggcc tgatatctct agaccgcggc tcgagcctct cgccctatag tgagtcgtat

      181 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg

      241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt

      301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag

      361 ggggaggtgt gggaggtttt ttaattcgcg gcgcgccgcg gcgccaatgc attgggcccg

      421 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca

      481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga

      541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg

      601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc

      661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac

      721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata

      781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa

      841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct

      901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa

      961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg

     1021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca

     1081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa

     1141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg

     1201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg

     1261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg

     1321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc

     1381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag

     1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac

     1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc

     1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag

     1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt

     1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag

     1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca

     1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact

     1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca

     1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg

     1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc

     2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg

     2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca

     2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt

     2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc

     2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc

     2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca

     2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa

     2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat

     2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa

     2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg

     2641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat

     2701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg

     2761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc

     2821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt

     2881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag

     2941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg

     3001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc

     3061 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag

     3121 ggcgatcggt gcgggcctct tcgctattac gccagtcgat cgaccatagc caattcaata

     3181 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata

     3241 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata

     3301 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga

     3361 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc

     3421 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat

     3481 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta

     3541 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc

     3601 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca

     3661 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg

     3721 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct

     3781 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca

     3841 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac

     3901 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaagtac

     3961 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc

     4021 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca

     4081 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta

     4141 gggaac

//

pCS111其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8