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首页    载体及质粒    pMHE7
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pMHE7

pMHE7

pMHE7



 

编号

载体名称

北京华越洋VECT75129

pMHE7

 

pMHE7载体基本信息:

载体名称:

pMHE7

质粒类型:

广宿主载体;细菌表达载体;体外表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

5920 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

T7 tagN-ter);FLAG-tagStrep-tag II (C-ter)

载体抗性:

链霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE7载体质粒图谱和多克隆位点信息:

pMHE7载体序列:

ORIGIN      

        1 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga

       61 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa

      121 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct

      181 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc

      241 tctagaaata attttgttta actttaagaa ggagatatac catggacgac tacaaggacg

      301 acgacgacaa gctcgagggc aactggagcc atccgcagtt cgagaagatc gagggaaggc

      361 ctgtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccggctgct

      421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa

      481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc

      541 ggatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat

      601 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc

      661 gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa

      721 acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag

      781 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg

      841 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg

      901 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc

      961 tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt

     1021 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat

     1081 agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca

     1141 acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc

     1201 gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc

     1261 ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg

     1321 cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg

     1381 gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc

     1441 ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt

     1501 gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc

     1561 ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga

     1621 aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg

     1681 cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt

     1741 gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga

     1801 ctgcgtggcc atgcttatct ccatgcggta gggtgccgca cggttgcggc accatgcgca

     1861 atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca

     1921 attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt

     1981 tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct

     2041 agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc

     2101 tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc

     2161 cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg

     2221 ggcaggcggg cgggcttcgc cttcgactgc ccccactcgc ataggcttgg gtcgttccag

     2281 gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg

     2341 gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa

     2401 ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt

     2461 cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg

     2521 tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc

     2581 gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga ccgcgcaggg

     2641 cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc gtaggcggtc acgactttgc

     2701 gaagcaaagt ctagtgagta tactcaagca ttgagtggcc cgccggaggc accgccttgc

     2761 gctgcccccg tcgagccggt tggacaccaa aagggagggg caggcatggc ggcatacgcg

     2821 atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg cggccagtct caagcacgcc

     2881 taccgcgagc gcgagacgcc caacgctgac gccagcagga cgccagagaa cgagcactgg

     2941 gcggccagca gcaccgatga agcgatgggc cgactgcgcg agttgctgcc agagaagcgg

     3001 cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg ccagcccgga atggtggaag

     3061 tcggccagcc aagaacagca ggcggcgttc ttcgagaagg cgcacaagtg gctggcggac

     3121 aagtacgggg cggatcgcat cgtgacggcc agcatccacc gtgacgaaac cagcccgcac

     3181 atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc tgtcggccaa ggagttcatc

     3241 ggcaacaaag cgcagatgac ccgcgaccag accacgtttg cggccgctgt ggccgatcta

     3301 gggctgcaac ggggcatcga gggcagcaag gcacgtcaca cgcgcattca ggcgttctac

     3361 gaggccctgg agcggccacc agtgggccac gtcaccatca gcccgcaagc ggtcgagcca

     3421 cgcgcctatg caccgcaggg attggccgaa aagctgggaa tctcaaagcg cgttgagacg

     3481 ccggaagccg tggccgaccg gctgacaaaa gcggttcggc aggggtatga gcctgcccta

     3541 caggccgccg caggagcgcg tgagatgcgc aagaaggccg atcaagccca agagacggcc

     3601 cgagaccttc gggagcgcct gaagcccgtt ctggacgccc tggggccgtt gaatcgggat

     3661 atgcaggcca aggccgccgc gatcatcaag gccgtgggcg aaaagctgct gacggaacag

     3721 cgggaagtcc agcgccagaa acaggcccag cgccagcagg aacgcgggcg cgcacatttc

     3781 cccgaaaagt gccacctggc ggcgttgtga caatttaccg aacaactccg cggccgggaa

     3841 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatag ctttatgctt

     3901 gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg gtccccaatt

     3961 aattagtaat ataatctatt aaaggtcatt caaaaggtca tccaccggat cagcttagta

     4021 aagccctcgc tagattttaa tgcggatgtt gcgattactt cgccaactat tgcgataaca

     4081 agaaaaagcc agcctttcat gatatatctc ccaatttgtg tagggcttat tatgcacgct

     4141 taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt

     4201 gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaattgttag

     4261 acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa

     4321 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc

     4381 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc

     4441 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac

     4501 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag

     4561 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac

     4621 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt

     4681 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc

     4741 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac

     4801 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa

     4861 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact

     4921 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg

     4981 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat

     5041 caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc

     5101 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc

     5161 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc

     5221 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta

     5281 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg

     5341 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc

     5401 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt

     5461 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag

     5521 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct

     5581 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg

     5641 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat

     5701 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc

     5761 cagcctgcgc gagcagggga attgatccgg tggatgacct tttgaatgac ctttaataga

     5821 ttatattact aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc

     5881 acaaaacggt ttacaagcat aaagctcaag gagatggcgc

//

 

 

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