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pMHE2

pMHE2

pMHE2



 

编号

载体名称

北京华越洋VECT75130

pMHE2

 

pMHE2载体基本信息:

载体名称:

pMHE2

质粒类型:

广宿主载体;细菌表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

T7/lac

载体大小:

5878 bp

5' 测序引物及序列:

T7 promoter 5’TAATACGACTCACTATAGGG 3’

3' 测序引物及序列:

T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’

载体标签:

6XHis tag (N ter)EK site

载体抗性:

链霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pMHE2载体质粒图谱和多克隆位点信息:


pMHE2载体序列:

ORIGIN      

        1 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac

       61 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat

      121 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga

      181 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga

      241 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatggggc

      301 atcatcatca tcatcatatc gagggaaggc ctgtcgacgg tatcgataag cttgatatcg

      361 aattcctgca gcccggggga tccggctgct aacaaagccc gaaaggaagc tgagttggct

      421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg

      481 ggttttttgc tgaaaggagg aactatatcc ggatcgccct tcccaacagt tgcgcagcct

      541 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt

      601 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa

      661 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata

      721 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca

      781 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag

      841 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga

      901 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa

      961 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg

     1021 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc

     1081 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac

     1141 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg

     1201 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg

     1261 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg

     1321 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc

     1381 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc

     1441 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc

     1501 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac

     1561 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt

     1621 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc

     1681 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt

     1741 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta

     1801 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa

     1861 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga

     1921 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt

     1981 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc

     2041 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg

     2101 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa

     2161 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc

     2221 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg

     2281 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc

     2341 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg

     2401 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct

     2461 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac

     2521 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg

     2581 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc

     2641 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca

     2701 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa

     2761 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg

     2821 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac

     2881 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc

     2941 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac

     3001 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc

     3061 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc

     3121 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag

     3181 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag

     3241 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag

     3301 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac

     3361 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa

     3421 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa

     3481 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc

     3541 aagaaggccg atcaagccca agagacggcc cgagaccttc gggagcgcct gaagcccgtt

     3601 ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag

     3661 gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag

     3721 cgccagcagg aacgcgggcg cgcacatttc cccgaaaagt gccacctggc ggcgttgtga

     3781 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt

     3841 ggcggtactt gggtcgatag ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa

     3901 taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt

     3961 caaaaggtca tccaccggat cagcttagta aagccctcgc tagattttaa tgcggatgtt

     4021 gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc

     4081 ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat

     4141 agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc

     4201 gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc

     4261 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc

     4321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag

     4381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct

     4441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg

     4501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg

     4561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt

     4621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc

     4681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat

     4741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga

     4801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac

     4861 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga

     4921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac

     4981 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt

     5041 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc

     5101 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca

     5161 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt

     5221 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc

     5281 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt

     5341 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt

     5401 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac

     5461 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt

     5521 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt

     5581 cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa

     5641 ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg

     5701 ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagggga attgatccgg

     5761 tggatgacct tttgaatgac ctttaataga ttatattact aattaattgg ggaccctaga

     5821 ggtccccttt tttattttaa aaattttttc acaaaacggt ttacaagcat aaagctca

//

 

 

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