北京华越洋生物
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首页    载体及质粒    pBTB-2
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pBTB-2

pBTB-2

pBTB-2



 

编号

载体名称

北京华越洋VECT75134

pBTB-2

 

pBTB-2载体基本信息:

载体名称:

pBTB-2

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

3740 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

卡那霉素

克隆菌株:

DH5α 等

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

                                                                                         

pBTB-2载体质粒图谱和多克隆位点信息:

 

pBTB-2载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc

     1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg

     1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg

     1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga

     1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact

     1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg

     1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg

     2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa

     2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca

     2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg

     2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt

     2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg

     2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga

     2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct

     2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt

     2521 tttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc

     2581 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac

     2641 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct

     2701 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa

     2761 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat

     2821 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg

     2881 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat

     2941 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa

     3001 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa

     3061 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact

     3121 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga

     3181 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct

     3241 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa

     3301 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg

     3361 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat

     3421 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact

     3481 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg

     3541 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc

     3601 acattgatta tttgcacggc gtcacacttt gctatgccat aacattttta tccataagat

     3661 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt

     3721 tttgggctag agaattcgtc

//

 

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