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pSPORT2

pSPORT2

pSPORT2



 

编号

载体名称

北京华越洋VECT75141

pSPORT2

 

pSPORT2载体基本信息:

载体名称:

pSPORT2

质粒类型:

原核表达载体;Cre/loxp载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

CMV

载体大小:

4311 bp

5' 测序引物及序列:

SP6 fwd 5’ATTTAGGTGACACTATAG 3’

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素

筛选标记:

--

克隆菌株:

DH5α

备注:

pSPORT2载体可用蓝白斑筛选法,筛选重组载体。

组成型/诱导型:

诱导型

病毒/非病毒:

非病毒

 

pSPORT2载体质粒图谱和多克隆位点信息:


 

 

pSPORT2载体序列:

ORIGIN

        1 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta

       61 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg

      121 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata

      181 gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gtcgacgagc

      241 tcactagtga cagcggccgc cccgggaatt ccggaccggt acctgcaggc gtaccagctt

      301 tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg

      361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc

      421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt

      481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg

      541 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct

      601 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc

      661 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt

      721 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa

      781 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga

      841 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt

      901 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac

      961 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca

     1021 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt

     1081 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt

     1141 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacccgtt

     1201 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg

     1261 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg

     1321 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg

     1381 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt

     1441 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat

     1501 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc

     1561 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga

     1621 tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat tgcaagctct gcattaatga

     1681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc

     1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg

     1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc

     1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc

     1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga

     1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc

     2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa

     2101 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg

     2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc

     2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga

     2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact

     2341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt

     2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag

     2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg

     2521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc ataacttcgt

     2581 atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt cacctagatc

     2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct

     2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca

     2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct

     2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca

     2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc

     2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg

     3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct

     3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa

     3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta

     3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc

     3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg

     3301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa

     3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg

     3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc

     3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg

     3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat

     3601 cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg atccctacac

     3661 agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat tctccctcca

     3721 gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg catgagcgga

     3781 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

     3841 aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt

     3901 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa

     3961 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag

     4021 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt

     4081 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac

     4141 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag

     4201 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg

     4261 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc c

//

 

pSPORT2其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8