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首页    载体及质粒    pBBR1MCS-6
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pBBR1MCS-6

pBBR1MCS-6

pBBR1MCS-6



 

编号

载体名称

北京华越洋VECT75143

pBBR1MCS-6

 

pBBR1MCS-6载体基本信息:

载体名称:

pBBR1MCS-6

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

lac

载体大小:

5859 bp

5' 测序引物及序列:

M13 Forward 5’TGTAAAACGACGGCCAGT 3’

3' 测序引物及序列:

M13 Reverse 5’CAGGAAACAGCTATGAC 3’

载体标签:

--

载体抗性:

链霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

可用蓝白斑筛选法

组成型/诱导型:

诱导型

 

pBBR1MCS-6载体质粒图谱和多克隆位点信息:


pBBR1MCS-6载体序列:

ORIGIN      

        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg

       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt

      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg

      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg

      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca

      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt

      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg

      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt

      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg

      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct

      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg

      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc

      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca

      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg

      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg

      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc

      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc

     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga

     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc

     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt

     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg

     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac

     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc

     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg

     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg

     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag

     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta

     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg

     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc

     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac

     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga

     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct

     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac

     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt

     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt

     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag

     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct

     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga

     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac

     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct

     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt

     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg

     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga

     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga

     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg

     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa

     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa

     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag

     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc

     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca

     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg

     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg

     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga

     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc

     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga

     3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct

     3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac

     3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac

     3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc

     3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcatt

     3601 catgtttgac agcttatcat ccataaggtt taatgcggta gtttatcaca gttaaattgc

     3661 taacgcagtc aggcaccgtg tatgaatcga actaatattt tttttggtga atcgcattct

     3721 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg

     3781 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac

     3841 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag

     3901 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg

     3961 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac

     4021 agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc

     4081 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt

     4141 acgccgctgc acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa

     4201 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac

     4261 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc

     4321 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa

     4381 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa

     4441 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg

     4501 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc

     4561 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta

     4621 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg

     4681 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc

     4741 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg

     4801 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga

     4861 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc

     4921 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac

     4981 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc

     5041 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct

     5101 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg

     5161 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt

     5221 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta

     5281 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct

     5341 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg

     5401 attccgcccc ttcccgcaaa cgaccccaaa caagatcttg atcccctgcg ccatcagatc

     5461 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc

     5521 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc

     5581 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag

     5641 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc

     5701 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct

     5761 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc

     5821 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggt

//

 

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