北京华越洋生物
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pBMTB-3

pBMTB-3

pBMTB-3

 



编号

载体名称

北京华越洋VECT75144

pBMTB-3

 

pBMTB-3载体基本信息:

载体名称:

pBMTB-3

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

4831 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氯霉素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBMTB-3载体质粒图谱和多克隆位点信息:

 

pBMTB-3载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt

      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct

      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct

      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa

      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc

      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt

      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt

      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt

      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct

      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct

      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct

      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg

      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga

      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt

      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc

      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt

     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca

     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt

     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg

     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc

     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc

     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt

     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag

     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc

     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc

     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg

     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc

     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt

     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc

     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg

     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa

     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc

     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt

     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta

     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa

     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc

     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa

     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt

     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg

     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga

     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga

     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca

     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc

     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc

     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt

     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc

     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga

     2881 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt

     2941 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac

     3001 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat

     3061 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct

     3121 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt

     3181 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca

     3241 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat

     3301 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa

     3361 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa

     3421 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg

     3481 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg

     3541 tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt

     3601 caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt cacaaccggc

     3661 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg

     3721 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag

     3781 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg

     3841 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc

     3901 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg

     3961 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg

     4021 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat

     4081 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc

     4141 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa

     4201 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc

     4261 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt

     4321 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg

     4381 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg

     4441 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat

     4501 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg

     4561 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct

     4621 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg

     4681 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt

     4741 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc

     4801 atacccgttt ttttgggcta gagaattcgt c

//

 

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