pNTAP-Shuttle-B
pNTAP-Shuttle-B
编号 | 载体名称 |
北京华越洋VECT10044 | pNTAP-Shuttle-B |
pNTAP-Shuttle-B载体基本信息:
载体名称: | pNTAP-Shuttle-B |
质粒类型: | 腺病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 7736 bp |
5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | SBP ; CBP (N-端) |
载体抗性: | 卡那霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | DH5α |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | 腺病毒载体pNTAP-Shuttle-B载体表达表达N端融合链霉亲和素肽和钙结合肽的目的蛋白。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
pNTAP-Shuttle-B载体质粒图谱和多克隆位点信息:
pNTAP-Shuttle-B载体简介:
pNTAP-Shuttle-B限制性酶切位点
pNTAP Shuttle B, 7736 bp version 016001
Enzymes with 1-10 cleavage sites #sites -- Bp position of recognition site --
AarI 1 3179
AatII 4 459, 512, 595, 781
Acc65I 1 1039
AccI 3 1202, 2816, 3754
AccIII 2 3909, 4136
AclI 1 6115
AcuI 6 1792, 1989, 3395, 5524, 6731
7163
AflIII 4 143, 1236, 3949, 4997
AhdI 2 2115, 5885
Alw44I 2 2499, 5311
AlwNI 5 1795, 1953, 2038, 5408, 6225
ApaI 2 2609, 2915
ApoI 7 242, 966, 1434, 2510, 3782
7271, 7282
AseI 3 4279, 6061, 7721
AvaI 6 1182, 1195, 1215, 1930, 2110
4214
AvaII 7 2114, 2138, 3163, 3304, 3382
6028, 7122
BamHI 2 1189, 4814
BanII 9 1153, 1631, 1688, 1844, 2609
2915, 3634, 6967, 7540
BbeI 3 3508, 6357, 6605
BbsI 2 1053, 3409
BciVI 3 2492, 5206, 6814
BclI 2 1277, 6447
BfrBI 3 2405, 2583, 4808
BglI 7 422, 544, 615, 1091, 1986
4064, 6004
BglII 1 934
BlnI 1 4324
Bme1580I 7 1019, 2499, 2609, 2915, 5311
6548, 6641
BmgBI 3 65, 2159, 4729
BmrI 5 632, 5935, 6230, 6311, 6545
BmtI 1 940
BpmI 5 1105, 1126, 1691, 4348, 5975
Bpu10I 3 1841, 2885, 2981
BpuEI 6 3373, 3763, 5103, 5365, 5642
6997
BsaAI 6 676, 1078, 2389, 6177, 6908
7467
BsaBI 4 1278, 2051, 4176, 6461
BsaHI 9 459, 512, 595, 781, 1199
2555, 3508, 6357, 6605
BsaI 5 1746, 2122, 3110, 3610, 5957
BsaWI 8 1261, 2495, 3909, 4136, 5203
5350, 6339, 6637
BseMII 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BseRI 3 3211, 3503, 4123
BseYI 9 1173, 2287, 2374, 2441, 2866
3063, 5301, 6352, 7110
BsgI 4 1861, 2471, 3526, 4482
BsiEI 7 1209, 1243, 2127, 4202, 4910
5334, 6512
BsiHKAI 9 1129, 1601, 1631, 2499, 3259
3634, 5311, 6715, 6905
BsmBI 3 978, 2206, 2553
BsmFI 8 499, 650, 818, 2113, 3011
3164, 3748, 6743
BsmI 4 1365, 1464, 2585, 3616
BspCNI 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BspHI 1 5717
BspMI 8 1976, 2820, 2878, 3180, 3899
6273, 6502, 6865
BsrBI 5 1212, 4345, 4928, 7217, 7611
BsrDI 4 2604, 5944, 6126, 6832
BsrGI 1 192
BssHII 5 2096, 2098, 3354, 3485, 7670
BssSI 4 3515, 3622, 5170, 7198
Bst1107I 1 3754
BstAPI 2 1854, 3073
BstEII 2 3176, 4092
BstXI 1 2593
Bsu36I 1 1138
BtgI 9 698, 1043, 1659, 1814, 2613
3292, 3444, 3468, 7038
BtgZI 7 687, 1885, 2829, 6892, 7043
7236, 7455
BtsI 5 1460, 1858, 2229, 3523, 6284
ClaI 1 1230
Csp6I 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
DdeI 10 981, 1139, 1842, 1951, 2525
2886, 2982, 5272, 5681, 5847
DraI 6 1320, 2017, 3775, 3999, 5754
5773
DraIII 2 1078, 7464
DrdI 4 3442, 5099, 6627, 7418
EagI 2 1209, 6512
EarI 3 4875, 6955, 7165
Eco47III 3 2284, 3399, 4364
EcoICRI 2 1631, 3634
EcoO109I 5 1088, 2137, 2340, 3303, 3597
EcoRI 1 3782
EcoRV 1 1249
FspI 2 6110, 6706
HgaI 10 855, 1200, 1205, 1238, 1673
2555, 3422, 5099, 5677, 7696
HincII 3 1202, 1381, 1895
HindIII 1 1224
HpaI 1 1381
KasI 3 3508, 6357, 6605
KpnI 1 1039
MluI 1 1236
MmeI 5 1766, 1961, 5187, 5371, 7421
MscI 3 268, 3360, 6686
MslI 5 699, 2594, 2659, 3880, 7039
MunI 2 1370, 1914
NaeI 4 1096, 4069, 7106, 7570
NarI 3 3508, 6357, 6605
NcoI 5 698, 1043, 1659, 3444, 7038
NdeI 2 571, 4819
NgoMIV 4 1096, 4069, 7106, 7570
NheI 1 940
NotI 1 1208
NsiI 3 2405, 2583, 4808
NspI 9 143, 1651, 3007, 3022, 3127
3949, 4806, 4997, 7007
PacI 2 4799, 7729
PciI 3 143, 3949, 4997
PfoI 2 2154, 2670
PmeI 1 3774
PmlI 1 1078
PpuMI 2 2137, 3303
PsiI 3 1401, 4041, 7339
PspOMI 2 2609, 2915
PstI 5 1774, 1801, 2231, 2964, 6131
PvuI 1 1243
PvuII 7 1111, 1956, 2864, 2891, 2967
6350, 6710
RsaI 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
RsrII 1 7121
SacI 2 1631, 3634
SacII 2 1814, 3468
SalI 1 1202
SapI 3 4874, 6955, 7165
SexAI 1 3659
SfcI 8 1774, 1801, 2231, 2964, 5262
5453, 6131, 7687
SfoI 3 3508, 6357, 6605
SgrAI 1 186
SmaI 5 1182, 1195, 1930, 2110, 4214
SmlI 7 1215, 3373, 3763, 5103, 5365
5642, 6997
SnaBI 1 676
SphI 3 1651, 3127, 7007
SrfI 1 1181
StyI 10 698, 1043, 1659, 2344, 3444
3480, 3498, 4324, 6415, 7038
TatI 5 192, 555, 635, 668, 719
TfiI 7 1920, 2461, 3569, 4176, 4972
7091, 7225
TspGWI 4 154, 3743, 4054, 4616
Tth111I 2 1748, 6721
Van91I 3 1058, 1077, 2155
XbaI 1 1267
XcmI 3 2037, 3628, 6415
XhoI 1 1215
XmaI 5 1182, 1195, 1930, 2110, 4214
XmnI 1 7719
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BsiWI BstBI EcoNI
FseI FspAI NruI PshAI SanDI
SbfI ScaI SfiI SpeI SspI
StuI SwaI
pNTAP-Shuttle-B载体序列:
PNTAP SHUTTLE B, 7736 BP VERSION 016001
NOTE: THE FOLLOWING SEQUENCE HAS BEEN VERIFIED FOR ACCURACY
AT THE JUNCTIONS. THE REMAINDER OF THE SEQUENCE HAS BEEN
OBTAINED FROM EXISTING DATA.
1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
51 GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
101 TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
151 GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
201 GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
251 CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
351 GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
401 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
451 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
501 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
551 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
601 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
651 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
701 TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
751 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
801 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
851 CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
901 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA
951 GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA
1001 AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC
1051 GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
1101 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC
1151 GGGAGCCCTC CGGCGGCTGC AAGCTGGGCT GCCCGGGCGG ATCCCCCGGG
1201 CGTCGACGCG GCCGCTCGAG CCGAAGCTTA TCGATACGCG TCCGATCGGA
1251 TATCCGATCC ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT
1301 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC
1351 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC
1401 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
1451 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
1501 TCTTAACGCG GATCTGGGCG TGGTTAAGGG TGGGAAAGAA TATATAAGGT
1551 GGGGGTCTTA TGTAGTTTTG TATCTGTTTT GCAGCAGCCG CCGCCGCCAT
1601 GAGCACCAAC TCGTTTGATG GAAGCATTGT GAGCTCATAT TTGACAACGC
1651 GCATGCCCCC ATGGGCCGGG GTGCGTCAGA ATGTGATGGG CTCCAGCATT
1701 GATGGTCGCC CCGTCCTGCC CGCAAACTCT ACTACCTTGA CCTACGAGAC
1751 CGTGTCTGGA ACGCCGTTGG AGACTGCAGC CTCCGCCGCC GCTTCAGCCG
1801 CTGCAGCCAC CGCCCGCGGG ATTGTGACTG ACTTTGCTTT CCTGAGCCCG
1851 CTTGCAAGCA GTGCAGCTTC CCGTTCATCC GCCCGCGATG ACAAGTTGAC
1901 GGCTCTTTTG GCACAATTGG ATTCTTTGAC CCGGGAACTT AATGTCGTTT
1951 CTCAGCAGCT GTTGGATCTG CGCCAGCAGG TTTCTGCCCT GAAGGCTTCC
2001 TCCCCTCCCA ATGCGGTTTA AAACATAAAT AAAAAACCAG ACTCTGTTTG
2051 GATTTGGATC AAGCAAGTGT CTTGCTGTCT TTATTTAGGG GTTTTGCGCG
2101 CGCGGTAGGC CCGGGACCAG CGGTCTCGGT CGTTGAGGGT CCTGTGTATT
2151 TTTTCCAGGA CGTGGTAAAG GTGACTCTGG ATGTTCAGAT ACATGGGCAT
2201 AAGCCCGTCT CTGGGGTGGA GGTAGCACCA CTGCAGAGCT TCATGCTGCG
2251 GGGTGGTGTT GTAGATGATC CAGTCGTAGC AGGAGCGCTG GGCGTGGTGC
2301 CTAAAAATGT CTTTCAGTAG CAAGCTGATT GCCAGGGGCA GGCCCTTGGT
2351 GTAAGTGTTT ACAAAGCGGT TAAGCTGGGA TGGGTGCATA CGTGGGGATA
2401 TGAGATGCAT CTTGGACTGT ATTTTTAGGT TGGCTATGTT CCCAGCCATA
2451 TCCCTCCGGG GATTCATGTT GTGCAGAACC ACCAGCACAG TGTATCCGGT
2501 GCACTTGGGA AATTTGTCAT GTAGCTTAGA AGGAAATGCG TGGAAGAACT
2551 TGGAGACGCC CTTGTGACCT CCAAGATTTT CCATGCATTC GTCCATAATG
2601 ATGGCAATGG GCCCACGGGC GGCGGCCTGG GCGAAGATAT TTCTGGGATC
2651 ACTAACGTCA TAGTTGTGTT CCAGGATGAG ATCGTCATAG GCCATTTTTA
2701 CAAAGCGCGG GCGGAGGGTG CCAGACTGCG GTATAATGGT TCCATCCGGC
2751 CCAGGGGCGT AGTTACCCTC ACAGATTTGC ATTTCCCACG CTTTGAGTTC
2801 AGATGGGGGG ATCATGTCTA CCTGCGGGGC GATGAAGAAA ACGGTTTCCG
2851 GGGTAGGGGA GATCAGCTGG GAAGAAAGCA GGTTCCTGAG CAGCTGCGAC
2901 TTACCGCAGC CGGTGGGCCC GTAAATCACA CCTATTACCG GGTGCAACTG
2951 GTAGTTAAGA GAGCTGCAGC TGCCGTCATC CCTGAGCAGG GGGGCCACTT
3001 CGTTAAGCAT GTCCCTGACT CGCATGTTTT CCCTGACCAA ATCCGCCAGA
3051 AGGCGCTCGC CGCCCAGCGA TAGCAGTTCT TGCAAGGAAG CAAAGTTTTT
3101 CAACGGTTTG AGACCGTCCG CCGTAGGCAT GCTTTTGAGC GTTTGACCAA
3151 GCAGTTCCAG GCGGTCCCAC AGCTCGGTCA CCTGCTCTAC GGCATCTCGA
3201 TCCAGCATAT CTCCTCGTTT CGCGGGTTGG GGCGGCTTTC GCTGTACGGC
3251 AGTAGTCGGT GCTCGTCCAG ACGGGCCAGG GTCATGTCTT TCCACGGGCG
3301 CAGGGTCCTC GTCAGCGTAG TCTGGGTCAC GGTGAAGGGG TGCGCTCCGG
3351 GCTGCGCGCT GGCCAGGGTG CGCTTGAGGC TGGTCCTGCT GGTGCTGAAG
3401 CGCTGCCGGT CTTCGCCCTG CGCGTCGGCC AGGTAGCATT TGACCATGGT
3451 GTCATAGTCC AGCCCCTCCG CGGCGTGGCC CTTGGCGCGC AGCTTGCCCT
3501 TGGAGGAGGC GCCGCACGAG GGGCAGTGCA GACTTTTGAG GGCGTAGAGC
3551 TTGGGCGCGA GAAATACCGA TTCCGGGGAG TAGGCATCCG CGCCGCAGGC
3601 CCCGCAGACG GTCTCGCATT CCACGAGCCA GGTGAGCTCT GGCCGTTCGG
3651 GGTCAAAAAC CAGGTTTCCC CCATGCTTTT TGATGCGTTT CTTACCTCTG
3701 GTTTCCATGA GCCGGTGTCC ACGCTCGGTG ACGAAAAGGC TGTCCGTGTC
3751 CCCGTATACA GACTTGAGAG GGAGTTTAAA CGAATTCAAT AGCTTGTTGC
3801 ATGGGCGGCG ATATAAAATG CAAGGTGCTG CTCAAAAAAT CAGGCAAAGC
3851 CTCGCGCAAA AAAGAAAGCA CATCGTAGTC ATGCTCATGC AGATAAAGGC
3901 AGGTAAGCTC CGGAACCACC ACAGAAAAAG ACACCATTTT TCTCTCAAAC
3951 ATGTCTGCGG GTTTCTGCAT AAACACAAAA TAAAATAACA AAAAAACATT
4001 TAAACATTAG AAGCCTGTCT TACAACAGGA AAAACAACCC TTATAAGCAT
4051 AAGACGGACT ACGGCCATGC CGGCGTGACC GTAAAAAAAC TGGTCACCGT
4101 GATTAAAAAG CACCACCGAC AGCTCCTCGG TCATGTCCGG AGTCATAATG
4151 TAAGACTCGG TAAACACATC AGGTTGATTC ACATCGGTCA GTGCTAAAAA
4201 GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT AGAGACAACA
4251 TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA
4301 TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC
4351 CAGAACAACA TACAGCGCTT CCACAGCGGC AGCCATAACA GTCAGCCTTA
4401 CCAGTAAAAA AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG
4451 CTCAATCAGT CACAGTGTAA AAAAGGGCCA AGTGCAGAGC GAGTATATAT
4501 AGGACTAAAA AATGACGTAA CGGTTAAAGT CCACAAAAAA CACCCAGAAA
4551 ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA CCCACAACTT
4601 CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTCACTT CCCATTTTAA
4651 GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG
4701 TCACCCGCCC CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT
4751 CATTATCATA TTGGCTTCAA TCCAAAATAA GGTATATTAT TGATGATGTT
4801 AATTAACATG CATGGATCCA TATGCGGTGT GAAATACCGC ACAGATGCGT
4851 AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT
4901 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
4951 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
5001 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
5051 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
5101 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
5151 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
5201 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
5251 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
5301 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
5351 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
5401 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
5451 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
5501 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
5551 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
5601 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
5651 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
5701 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
5751 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
5801 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
5851 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
5901 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
5951 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
6001 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
6051 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
6101 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGCCAT GAGATTATCA
6151 AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA GTCCGCAGAA
6201 ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG
6251 AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG
6301 CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC
6351 AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA
6401 TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGCTCTGAT
6451 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
6501 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG
6551 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
6601 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
6651 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG
6701 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
6751 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
6801 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA
6851 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
6901 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
6951 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA
7001 AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC
7051 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
7101 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
7151 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
7201 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
7251 CCTTCTTGAC GAGTTCTTCT GAATTTTGTT AAAATTTTTG TTAAATCAGC
7301 TCATTTTTTA ACCAATAGGC CGAAATCGGC ACCATCCCTT ATAAATCAAA
7351 AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
7401 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT
7451 CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGTG
7501 GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT
7551 TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG
7601 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT
7651 GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
7701 CATTCGCCAT TCAGGATCGA ATTAATTCTT AATTAA
pNTAP-Shuttle-B其他腺病毒表达载体: