pAdTrack-CMV
pAdTrack-CMV
pAdTrack-CMV
编号 | 载体名称 |
北京华越洋VECT10010 | pAdTrack-CMV |
pAdTrack-CMV载体基本信息:
载体名称: | pAdTrack-CMV, pAdTrack CMV, pAdTrackCMV |
质粒类型: | 哺乳动物细胞腺病毒表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 9220 bp |
5' 测序引物及序列: | / |
3' 测序引物及序列: | / |
载体标签: | EGFP |
载体抗性: | 卡那 |
备注: | pAdTrack-CMV载体用来转基因腺病毒细胞表达，载体拥有CMV启动子和EGFP绿色荧光蛋白跟踪基因表达结果，和pAdeasy1载体共同使用时最大能够插入 5.9kb，pAdeasy2载体共同使用时能够最大插入8.6kb片段。具体使用方法可以参考pAdeasy载体系统。 |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 腺病毒 |
pAdTrack-CMV载体质粒图谱和多克隆位点信息:
pAdTrack-CMV载体序列:
ORIGIN
1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA
61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GCGTGGGAAC GGGGCGGGTG ACGTAGTAGT
121 GTGGCGGAAG TGTGATGTTG CAAGTGTGGC GGAACACATG TAAGCGACGG ATGTGGCAAA
181 AGTGACGTTT TTGGTGTGCG CCGGTGTACA CAGGAAGTGA CAATTTTCGC GCGGTTTTAG
241 GCGGATGTTG TAGTAAATTT GGGCGTAACC GAGTAAGATT TGGCCATTTT CGCGGGAAAA
301 CTGAATAAGA GGAAGTGAAA TCTGAATAAT TTTGTGTTAC TCATAGCGCG TAANNNCGCG
361 TTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
421 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
481 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT
541 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA
601 TCCGGTGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC
661 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC
721 GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT
781 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT
841 GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT
901 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT
961 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA
1021 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA
1081 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA
1141 GGTCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA
1201 CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT
1261 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC
1321 CTCGCCCTTG CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA
1381 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
1441 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA
1501 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
1561 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
1621 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
1681 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
1741 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT
1801 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
1861 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT
1921 GACCCCGTAA TTGATTACTA TTANNNCGCG TTAAGATACA TTGATGAGTT TGGACAAACC
1981 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
2041 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
2101 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
2161 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CTGAGTCCGG ACTTGTACAG
2221 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCANNGATC GGATATCTTA
2281 TCTAGAAGCT TAGGCTCGAG CGGCCGCGTC GACGGTACCA GATCTCTAGC GGATCTGACG
2341 GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG
2401 CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA
2461 CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA
2521 TCCACGCCCA TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC
2581 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC
2641 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG
2701 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC
2761 TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG
2821 CGGTCAGCCA GGCGGGCCAT TTACCAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG
2881 ACCCCGTAAT TGATTACTAT TANNNTAAGG GTGGGAAAGA ATATATAAGG TGGGGGTCTT
2941 ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA TGAGCACCAA CTCGTTTGAT
3001 GGAAGCATTG TGAGCTCATA TTTGACAACG CGCATGCCCC CATGGGCCGG GGTGCGTCAG
3061 AATGTGATGG GCTCCAGCAT TGATGGTCGC CCCGTCCTGC CCGCAAACTC TACTACCTTG
3121 ACCTACGAGA CCGTGTCTGG AACGCCGTTG GAGACTGCAG CCTCCGCCGC CGCTTCAGCC
3181 GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT TCCTGAGCCC GCTTGCAAGC
3241 AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA CGGCTCTTTT GGCACAATTG
3301 GATTCTTTGA CCCGGGAACT TAATGTCGTT TCTCAGCAGC TGTTGGATCT GCGCCAGCAG
3361 GTTTCTGCCC TGAAGGCTTC CTCCCCTCCC AATGCGGTTT AAAACATAAA TAAAAAACCA
3421 GACTCTGTTT GGATTTGGAT CAAGCAAGTG TCTTGCTGTC TTTATTTAGG GGTTTTGCGC
3481 GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG TCCTGTGTAT TTTTTCCAGG
3541 ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA TAAGCCCGTC TCTGGGGTGG
3601 AGGTAGCACC ACTGCAGAGC TTCATGCTGC GGGGTGGTGT TGTAGATGAT CCAGTCGTAG
3661 CAGGAGCGCT GGGCGTGGTG CCTAAAAATG TCTTTCAGTA GCAAGCTGAT TGCCAGGGGC
3721 AGGCCCTTGG TGTAAGTGTT TACAAAGCGG TTAAGCTGGG ATGGGTGCAT ACGTGGGGAT
3781 ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT TCCCAGCCAT ATCCCTCCGG
3841 GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG TGCACTTGGG AAATTTGTCA
3901 TGTAGCTTAG AAGGAAATGC GTGGAAGAAC TTGGAGACGC CCTTGTGACC TCCAAGATTT
3961 TCCATGCATT CGTCCATAAT GATGGCAATG GGCCCACGGG CGGCGGCCTG GGCGAAGATA
4021 TTTCTGGGAT CACTAACGTC ATAGTTGTGT TCCAGGATGA GATCGTCATA GGCCATTTTT
4081 ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG TTCCATCCGG CCCAGGGGCG
4141 TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT CAGATGGGGG GATCATGTCT
4201 ACCTGCGGGG CGATGAAGAA AACGGTTTCC GGGGTAGGGG AGATCAGCTG GGAAGAAAGC
4261 AGGTTCCTGA GCAGCTGCGA CTTACCGCAG CCGGTGGGCC CGTAAATCAC ACCTATTACC
4321 GGGTGCAACT GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT CCCTGAGCAG GGGGGCCACT
4381 TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA AATCCGCCAG AAGGCGCTCG
4441 CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT TCAACGGTTT GAGACCGTCC
4501 GCCGTAGGCA TGCTTTTGAG CGTTTGACCA AGCAGTTCCA GGCGGTCCCA CAGCTCGGTC
4561 ACCTGCTCTA CGGCATCTCG ATCCAGCATA TCTCCTCGTT TCGCGGGTTG GGGCGGCTTT
4621 CGCTGTACGG CAGTAGTCGG TGCTCGTCCA GACGGGCCAG GGTCATGTCT TTCCACGGGC
4681 GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG GTGCGCTCCG GGCTGCGCGC
4741 TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA GCGCTGCCGG TCTTCGCCCT
4801 GCGCGTCGGC CAGGTAGCAT TTGACCATGG TGTCATAGTC CAGCCCCTCC GCGGCGTGGC
4861 CCTTGGCGCG CAGCTTGCCC TTGGAGGAGG CGCCGCACGA GGGGCAGTGC AGACTTTTGA
4921 GGGCGTAGAG CTTGGGCGCG AGAAATACCG ATTCCGGGGA GTAGGCATCC GCGCCGCAGG
4981 CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC TGGCCGTTCG GGGTCAAAAA
5041 CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT GGTTTCCATG AGCCGGTGTC
5101 CACGCTCGGT GACGAAAAGG CTGTCCGTGT CCCCGTATAC AGACTNNNGT TTAAACGAAT
5161 TCNNNTATAA AATGCAAGGT GCTGCTCAAA AAATCAGGCA AAGCCTCGCG CAAAAAAGAA
5221 AGCACATCGT AGTCATGCTC ATGCAGATAA AGGCAGGTAA GCTCCGGAAC CACCACAGAA
5281 AAAGACACCA TTTTTCTCTC AAACATGTCT GCGGGTTTCT GCATAAACAC AAAATAAAAT
5341 AACAAAAAAA CATTTAAACA TTAGAAGCCT GTCTTACAAC AGGAAAAACA ACCCTTATAA
5401 GCATAAGACG GACTACGGCC ATGCCGGCGT GACCGTAAAA AAACTGGTCA CCGTGATTAA
5461 AAAGCACCAC CGACAGCTCC TCGGTCATGT CCGGAGTCAT AATGTAAGAC TCGGTAAACA
5521 CATCAGGTTG ATTCATCGGT CAGTGCTAAA AAGCGACCGA AATAGCCCGG GGGAATACAT
5581 ACCCGCAGGC GTAGAGACAA CATTACAGCC CCCATAGGAG GTATAACAAA ATTAATAGGA
5641 GAGAAAAACA CATAAACACC TGAAAAACCC TCCTGCCTAG GCAAAATAGC ACCCTCCCGC
5701 TCCAGAACAA CATACAGCGC TTCACAGCGG CAGCCTAACA GTCAGCCTTA CCAGTAAAAA
5761 AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG CTCAATCAGT CACAGTGTAA
5821 AAAAGGGCCA AGTGCAGAGC GAGTATATAT AGGACTAAAA AATGACGTAA CGGTTAAAGT
5881 CCACAAAAAA CACCCAGAAA ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA
5941 CCCACAACTT CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTAACTT CCCATTTTAA
6001 GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG TCACCCGCCC
6061 CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT CATTATCATA TTGGCTTCAA
6121 TCCAAAATAA GGTATATTAT TGATGATNNN TTAATTAAGG ATCCNNNCGG TGTGAAATAC
6181 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG
6241 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
6301 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
6361 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
6421 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
6481 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
6541 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
6601 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
6661 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
6721 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
6781 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
6841 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
6901 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
6961 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
7021 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
7081 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
7141 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
7201 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
7261 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
7321 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
7381 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
7441 CAGTTAATAG TTTGCGCAAC GTTGTTGNNN NNNAAAAAGG ATCTTCACCT AGATCCTTTT
7501 CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC
7561 TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC
7621 ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG
7681 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTCGCCGCC
7741 AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG
7801 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
7861 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
7921 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
7981 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
8041 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
8101 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
8161 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
8221 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
8281 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
8341 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
8401 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
8461 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
8521 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
8581 TGACGAGTTC TTCTGAATTT TGTTAAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT
8641 AGGCCGAAAT CGGCAACATC CCTTATAAAT CAAAAGAATA GACCGCGATA GGGTTGAGTG
8701 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC
8761 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT
8821 TGCGGTCGAG GTGCCGTAAA GCTCTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG
8881 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG
8941 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCGCGCT
9001 TAATGCGCCG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
9061 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
9121 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
9181 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
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pAdTrack-CMV其他腺病毒表达载体：
