北京华越洋生物
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14

pDC311

pDC311

pDC311

 

编号

载体名称

北京华越洋VECT10047

pDC311

 

pDC311载体基本信息:

载体名称:

pDC311

质粒类型:

腺病毒表达载体

高拷贝/低拷贝:

--

启动子:

MCMV

克隆方法:

--

载体大小:

3276 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

克隆宿主菌:DB3.1

稳定性:

--

组成型:

组成型

病毒/非病毒:

腺病毒

 

pDC311载体质粒图谱和多克隆位点信息:

 

pDC311载体序列:

ORIGIN

        1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT

       61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG

      121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG

      181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT

      241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA

      301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG

      361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC

      421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGNTCTAGA GAATTCAGGC CTGCTAGCAG

      481 ATCTAAGCTT GAGCTCGTCG ACTCGAAGAT CCAATAACTT CGTATAGCAT ACATTATACG

      541 AAGTTATAAG TGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA

      601 GCCGGGCCAC CTCGACCTGA ATGGAAGCCG GCGGCACCTC GCTAACGGAT TCACCACTCC

      661 AAGAATTGGA GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA

      721 GAACATATCC ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCT CGGCTGCATT

      781 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT

      841 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA

      901 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA

      961 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC

     1021 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

     1081 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC

     1141 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT

     1201 CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT

     1261 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG

     1321 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

     1381 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT

     1441 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA

     1501 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT

     1561 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA

     1621 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

     1681 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA

     1741 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT

     1801 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA

     1861 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT

     1921 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

     1981 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA

     2041 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT

     2101 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA

     2161 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA

     2221 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

     2281 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT

     2341 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAACACGG GATAATACCG

     2401 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC

     2461 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT

     2521 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

     2581 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT

     2641 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT

     2701 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG

     2761 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACTCTAG

     2821 GCAAAATAGC ACCCTCCCGC TCCAGAACAA CATACAGCGC TTCACAGCGG CAGCCTAACA

     2881 GTCAGCCTTA CCAGTAAAAA AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG

     2941 CTCAATCAGT CACAGTGTAA AAAAGGGCCA AGTGCAGAGC GAGTATATAT AGGACTAAAA

     3001 AATGACGTAA CGGTTAAAGT CCACAAAAAA CACCCAGAAA ACCGCACGCG AACCTACGCC

     3061 CAGAAACGAA AGCCAAAAAA CCCACAACTT CCTCAAATCG TCACTTCCGT TTTCCCACGT

     3121 TACGTAACTT CCCATTTTAA GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT

     3181 AAAACCTACG TCACCCGCCC CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT

     3241 CATTATCATA TTGGCTTCAA TCCAAAATAA GGTATA

//

 

pDC311其他腺病毒表达载体:

pNTAP-Shuttle-B

pAAV-CAG-RFP

pAAV-CaMKIIa-EGFP

pCTAP-Shuttle-A

pAAV-CMV-Rluc

pNTAP-Shuttle-A

pAAV-CAG-GFP

pBHGloxdelE13cre

pCTAP-Shuttle-C

pDC511

pDC515

pDC312

pAdTrack-CMV

pAAV-IRES-hrGFP

pShuttle-IRES-hrGFP-1

pShuttle-CMV-EGFP-C

pacAd5 9.2-100

pacAd5 CMV-GFP

pAAV-Syn-Rluc

pCMV-MCS

pShuttle-IRES-hrGFP-2

pShuttle-CMV

pAAV-Syn-Rluc

pAAV-hSyn-RFP

pAAV-CMV-iRFP

pAAV-UbC

pDC415

pAAV-CAG-Fluc

pAAV-LacZ

pNTAP-Shuttle-B

pDC316

pDC516

pAAV-CA

pDC416

pNTAP-Shuttle-C

pDC311

pDC512

pAAV-hrGFP

pDC411

pAAV-RC

pAdTrack

pacAd5 CMVK-NpA

pAdEasy-1

pDC315

pAAV-MCS

pShuttle

pHelper

pBApo-CMV-neo

pShuttle-CMV-lacZ

pBApo-CMV

pAAV-TRE-Syn-Fluc

pBApo-CMV-Pur

pAAV-minCMV-mCherry