pShuttle

pShuttle

pShuttle载体基本信息：

pShuttle载体质粒图谱和多克隆位点信息：

pShuttle载体简介：

pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.

pShuttle载体序列：

ORIGIN     

        1 TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT

       61 GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA

      121 CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG

      181 AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT

      241 AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG

      301 TGTTACTCAT AGCGCGTAAT ACTGGTACCG CGGCCGCCTC GAGTCTAGAG ATATCGAATT

      361 CAAGCTTGTC GACTCGAAGA TCTGGGCGTG GTTAAGGGTG GGAAAGAATA TATAAGGTGG

      421 GGGTCTTATG TAGTTTTGTA TCTGTTTTGC AGCAGCCGCC GCCGCCATGA GCACCAACTC

      481 GTTTGATGGA AGCATTGTGA GCTCATATTT GACAACGCGC ATGCCCCCAT GGGCCGGGGT

      541 GCGTCAGAAT GTGATGGGCT CCAGCATTGA TGGTCGCCCC GTCCTGCCCG CAAACTCTAC

      601 TACCTTGACC TACGAGACCG TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT CCGCCGCCGC

      661 TTCAGCCGCT GCAGCCACCG CCCGCGGGAT TGTGACTGAC TTTGCTTTCC TGAGCCCGCT

      721 TGCAAGCAGT GCAGCTTCCC GTTCATCCGC CCGCGATGAC AAGTTGACGG CTCTTTTGGC

      781 ACAATTGGAT TCTTTGACCC GGGAACTTAA TGTCGTTTCT CAGCAGCTGT TGGATCTGCG

      841 CCAGCAGGTT TCTGCCCTGA AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA ACATAAATAA

      901 AAAACCAGAC TCTGTTTGGA TTTGGATCAA GCAAGTGTCT TGCTGTCTTT ATTTAGGGGT

      961 TTTGCGCGCG CGGTAGGCCC GGGACCAGCG GTCTCGGTCG TTGAGGGTCC TGTGTATTTT

     1021 TTCCAGGACG TGGTAAAGGT GACTCTGGAT GTTCAGATAC ATGGGCATAA GCCCGTCTCT

     1081 GGGGTGGAGG TAGCACCACT GCAGAGCTTC ATGCTGCGGG GTGGTGTTGT AGATGATCCA

     1141 GTCGTAGCAG GAGCGCTGGG CGTGGTGCCT AAAAATGTCT TTCAGTAGCA AGCTGATTGC

     1201 CAGGGGCAGG CCCTTGGTGT AAGTGTTTAC AAAGCGGTTA AGCTGGGATG GGTGCATACG

     1261 TGGGGATATG AGATGCATCT TGGACTGTAT TTTTAGGTTG GCTATGTTCC CAGCCATATC

     1321 CCTCCGGGGA TTCATGTTGT GCAGAACCAC CAGCACAGTG TATCCGGTGC ACTTGGGAAA

     1381 TTTGTCATGT AGCTTAGAAG GAAATGCGTG GAAGAACTTG GAGACGCCCT TGTGACCTCC

     1441 AAGATTTTCC ATGCATTCGT CCATAATGAT GGCAATGGGC CCACGGGCGG CGGCCTGGGC

     1501 GAAGATATTT CTGGGATCAC TAACGTCATA GTTGTGTTCC AGGATGAGAT CGTCATAGGC

     1561 CATTTTTACA AAGCGCGGGC GGAGGGTGCC AGACTGCGGT ATAATGGTTC CATCCGGCCC

     1621 AGGGGCGTAG TTACCCTCAC AGATTTGCAT TTCCCACGCT TTGAGTTCAG ATGGGGGGAT

     1681 CATGTCTACC TGCGGGGCGA TGAAGAAAAC GGTTTCCGGG GTAGGGGAGA TCAGCTGGGA

     1741 AGAAAGCAGG TTCCTGAGCA GCTGCGACTT ACCGCAGCCG GTGGGCCCGT AAATCACACC

     1801 TATTACCGGG TGCAACTGGT AGTTAAGAGA GCTGCAGCTG CCGTCATCCC TGAGCAGGGG

     1861 GGCCACTTCG TTAAGCATGT CCCTGACTCG CATGTTTTCC CTGACCAAAT CCGCCAGAAG

     1921 GCGCTCGCCG CCCAGCGATA GCAGTTCTTG CAAGGAAGCA AAGTTTTTCA ACGGTTTGAG

     1981 ACCGTCCGCC GTAGGCATGC TTTTGAGCGT TTGACCAAGC AGTTCCAGGC GGTCCCACAG

     2041 CTCGGTCACC TGCTCTACGG CATCTCGATC CAGCATATCT CCTCGTTTCG CGGGTTGGGG

     2101 CGGCTTTCGC TGTACGGCAG TAGTCGGTGC TCGTCCAGAC GGGCCAGGGT CATGTCTTTC

     2161 CACGGGCGCA GGGTCCTCGT CAGCGTAGTC TGGGTCACGG TGAAGGGGTG CGCTCCGGGC

     2221 TGCGCGCTGG CCAGGGTGCG CTTGAGGCTG GTCCTGCTGG TGCTGAAGCG CTGCCGGTCT

     2281 TCGCCCTGCG CGTCGGCCAG GTAGCATTTG ACCATGGTGT CATAGTCCAG CCCCTCCGCG

     2341 GCGTGGCCCT TGGCGCGCAG CTTGCCCTTG GAGGAGGCGC CGCACGAGGG GCAGTGCAGA

     2401 CTTTTGAGGG CGTAGAGCTT GGGCGCGAGA AATACCGATT CCGGGGAGTA GGCATCCGCG

     2461 CCGCAGGCCC CGCAGACGGT CTCGCATTCC ACGAGCCAGG TGAGCTCTGG CCGTTCGGGG

     2521 TCAAAAACCA GGTTTCCCCC ATGCTTTTTG ATGCGTTTCT TACCTCTGGT TTCCATGAGC

     2581 CGGTGTCCAC GCTCGGTGAC GAAAAGGCTG TCCGTGTCCC CGTATACAGA CTNNNGTTTA

     2641 AACGAATTCN NNTATAAAAT GCAAGGTGCT GCTCAAAAAA TCAGGCAAAG CCTCGCGCAA

     2701 AAAAGAAAGC ACATCGTAGT CATGCTCATG CAGATAAAGG CAGGTAAGCT CCGGAACCAC

     2761 CACAGAAAAA GACACCATTT TTCTCTCAAA CATGTCTGCG GGTTTCTGCA TAAACACAAA

     2821 ATAAAATAAC AAAAAAACAT TTAAACATTA GAAGCCTGTC TTACAACAGG AAAAACAACC

     2881 CTTATAAGCA TAAGACGGAC TACGGCCATG CCGGCGTGAC CGTAAAAAAA CTGGTCACCG

     2941 TGATTAAAAA GCACCACCGA CAGCTCCTCG GTCATGTCCG GAGTCATAAT GTAAGACTCG

     3001 GTAAACACAT CAGGTTGATT CATCGGTCAG TGCTAAAAAG CGACCGAAAT AGCCCGGGGG

     3061 AATACATACC CGCAGGCGTA GAGACAACAT TACAGCCCCC ATAGGAGGTA TAACAAAATT

     3121 AATAGGAGAG AAAAACACAT AAACACCTGA AAAACCCTCC TGCCTAGGCA AAATAGCACC

     3181 CTCCCGCTCC AGAACAACAT ACAGCGCTTC ACAGCGGCAG CCTAACAGTC AGCCTTACCA

     3241 GTAAAAAAGA AAACCTATTA AAAAAACACC ACTCGACACG GCACCAGCTC AATCAGTCAC

     3301 AGTGTAAAAA AGGGCCAAGT GCAGAGCGAG TATATATAGG ACTAAAAAAT GACGTAACGG

     3361 TTAAAGTCCA CAAAAAACAC CCAGAAAACC GCACGCGAAC CTACGCCCAG AAACGAAAGC

     3421 CAAAAAACCC ACAACTTCCT CAAATCGTCA CTTCCGTTTT CCCACGTTAC GTAACTTCCC

     3481 ATTTTAAGAA AACTACAATT CCCAACACAT ACAAGTTACT CCGCCCTAAA ACCTACGTCA

     3541 CCCGCCCCGT TCCCACGCCC CGCGCCACGT CACAAACTCC ACCCCCTCAT TATCATATTG

     3601 GCTTCAATCC AAAATAAGGT ATATTATTGA TGATNNNTTA ATTAAGGATC CNNNCGGTGT

     3661 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG

     3721 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG

     3781 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA

     3841 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC

     3901 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA

     3961 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG

     4021 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT

     4081 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT

     4141 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG

     4201 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC

     4261 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC

     4321 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA

     4381 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC

     4441 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG

     4501 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA

     4561 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT

     4621 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA

     4681 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG

     4741 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA

     4801 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT

     4861 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT

     4921 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGNNNNNN AAAAAGGATC TTCACCTAGA

     4981 TCCTTTTCAC GTAGAAAGCC AGTCCGCAGA AACGGTGCTG ACCCCGGATG AATGTCAGCT

     5041 ACTGGGCTAT CTGGACAAGG GAAAACGCAA GCGCAAAGAG AAAGCAGGTA GCTTGCAGTG

     5101 GGCTTACATG GCGATAGCTA GACTGGGCGG TTTTATGGAC AGCAAGCGAA CCGGAATTGC

     5161 CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA AGTAAACTGG ATGGCTTTCT

     5221 CGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGCTCTGA TCAAGAGACA GGATGAGGAT

     5281 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA

     5341 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC

     5401 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA

     5461 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG

     5521 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC

     5581 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG

     5641 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA

     5701 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC

     5761 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA

     5821 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG

     5881 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT

     5941 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG

     6001 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC

     6061 GCCTTCTTGA CGAGTTCTTC TGAATTTTGT TAAAATTTTT GTTAAATCAG CTCATTTTTT

     6121 AACCAATAGG CCGAAATCGG CAACATCCCT TATAAATCAA AAGAATAGAC CGCGATAGGG

     6181 TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC

     6241 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCAAATCA

     6301 AGTTTTTTGC GGTCGAGGTG CCGTAAAGCT CTAAATCGGA ACCCTAAAGG GAGCCCCCGA

     6361 TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA

     6421 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC

     6481 GCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCATTCGCCA TTCAGGATCG AATTAATTCT

     6541 TAATTAACAT CATCAATAAT ATACCTTA

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pShuttle其他腺病毒表达载体：