pMT/BioEase-DEST
pMT/BioEase-DEST
编号 | 载体名称 |
北京华越洋生物 KCVECT100 | pMT/BioEase-DEST |
pMT/BioEase-DEST载体基本信息
载体名称: | pMT/BioEase-DEST |
质粒类型: | 昆虫表达载体；Gateway载体；分泌表达载体 |
克隆方法: | Gateway |
启动子: | MT |
载体大小: | 5375 bp |
5' 测序引物及序列: | MT Forward：CATCTCAGTGCAACTAAA |
3' 测序引物及序列: | -- |
载体标签: | ioEase Tag（N-末端） |
载体抗性: | 氨苄青霉素、氯霉素（仅空载体） |
真核筛选标记: | 无 |
克隆菌株: | DB3.1(载体复制）；TOP10；DH5-T1R |
宿主细胞（系）: | 果蝇细胞系S2 |
备注: | 与pCoBlast或者pCoHygro共转染，可筛选建立稳定表达的细胞系。 |
瞬表达/稳表达: | 瞬表达 |
组成型/诱导型: | 诱导型（硫酸铜或氯化镉） |
病毒/非病毒: | 非病毒 |
pMT/BioEase-DEST载体质粒图谱和多克隆位点信息
pMT/BioEase-DEST载体简介
The DES BioEase Expression Kit with pCoBlast provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pMT/BioEase-DEST vector incorporates a 72-amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. Proteins produced in pMT/BioEase-DEST are expressed as fusion proteins to this sequence. Biotinylated fusion proteins can be purified on streptavidin agarose or detected on western blots using streptavidin-HRP or streptavidin-AP conjugates. In addition to the BioEase tag, the pMT/BioEase-DEST vector offers the following features: attR sites for fast and efficient recombination with any attL-flanked Gateway vector metallothionein (MT) promoter for high level, inducible expression in S2 cells Enterokinase (EK) cleavage site for efficient removal of the N-terminal BioEasefusion tag A Blasticidin vector (pCoBlast) for rapid selection of stable cells
pMT/BioEase-DEST载体序列
ORIGIN
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAG TGAATTAATT CGTTGCAGGA
421 CAGGATGTGG TGCCCGATGT GACTAGCTCT TTGCTGCAGG CCGTCCTATC CTCTGGTTCC
481 GATAAGAGAC CCAGAACTCC GGCCCCCCAC CGCCCACCGC CACCCCCATA CATATGTGGT
541 ACGCAAGTAA GAGTGCCTGC GCATGCCCCA TGTGCCCCAC CAAGAGTTTT GCATCCCATA
601 CAAGTCCCCA AAGTGGAGAA CCGAACCAAT TCTTCGCGGG CAGAACAAAA GCTTCTGCAC
661 ACGTCTCCAC TCGAATTTGG AGCCGGCCGG CGTGTGCAAA AGAGGTGAAT CGAACGAAAG
721 ACCCGTGTGT AAAGCCGCGT TTCCAAAATG TATAAAACCG AGAGCATCTG GCCAATGTGC
781 ATCAGTTGTG GTCAGCAGCA AAATCAAGTG AATCATCTCA GTGCAACTAA AGGGGGGATC
841 TAGCGTTTAA ACTTAAGCTT ACCATGGGCG CCGGCACCCC GGTGACCGCC CCGCTGGCGG
901 GCACTATCTG GAAGGTGCTG GCCAGCGAAG GCCAGACGGT GGCCGCAGGC GAGGTGCTGC
961 TGATTCTGGA AGCCATGAAG ATGGAAACCG AAATCCGCGC CGCGCAGGCC GGGACCGTGC
1021 GCGGTATCGC GGTGAAAGCC GGCGACGCGG TGGCGGTCGG CGACACCCTG ATGACCCTGG
1081 CGGGCTCTGG ATCCGATCTG TACGACGATG ACGATAAGGT ACATCAAACA AGTTTGTACA
1141 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC
1201 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC
1261 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG
1321 TTAGGATCCG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG
1381 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC
1441 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC
1501 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT
1561 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG
1621 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG
1681 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA
1741 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC
1801 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT
1861 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT
1921 GGCGATTCAG GTTCATCATG CCGTCTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA
1981 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA ACGCGTGGAT CCGGCTTACT
2041 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTCGCGAA CCGGTGTATA CCCGAAGTAT
2101 GTCAAAAAGA GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
2161 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA
2221 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
2281 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA
2341 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
2401 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
2461 CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
2521 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
2581 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
2641 ATATAAATGT CAGGCTCCGT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT
2701 ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT
2761 GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TGATAATTAA
2821 TTAAGATCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAAGATC
2881 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA
2941 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA
3001 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT
3061 GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT
3121 CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
3181 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG
3241 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
3301 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
3361 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
3421 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
3481 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
3541 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
3601 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
3661 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
3721 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
3781 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
3841 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
3901 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
3961 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
4021 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
4081 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
4141 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
4201 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
4261 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
4321 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
4381 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
4441 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
4501 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
4561 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
4621 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
4681 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
4741 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
4801 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
4861 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
4921 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
4981 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
5041 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
5101 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
5161 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
5221 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
5281 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
5341 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGT
//
pMT/BioEase-DEST其他昆虫表达载体：
