北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pXINSECT-DEST38
1

pXINSECT-DEST38

pXINSECT-DEST38

pXINSECT-DEST38

 

编号

载体名称

北京华越洋生物

KCVECT101

pXINSECT-DEST38

pXINSECT-DEST38载体基本信息

载体名称:

pXINSECT-DEST38

质粒类型:

昆虫表达载体;Gateway载体

克隆方法:

Gateway

启动子:

Actin

载体大小:

12419 kb

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

无标签

载体抗性:

氨苄青霉素、氯霉素(仅空载体)

真核筛选标记:

克隆菌株:

DB3.1

宿主细胞(系):

High Five(推荐) 等细胞系

备注:

pXINSECT-DEST38载体与pBmA-neo载体共转染,可以建立稳表达细胞系;
 
应用Gateway技术,可快速高效将 目的基因片段(尤其适用于目的基因片段>1kb,用传统的连接酶法效率低的)插入到载体中;

瞬表达/稳表达:

瞬表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

pXINSECT-DEST38载体质粒图谱和多克隆位点信息

 

pXINSECT-DEST38载体简介

Using the Express Insect Kit or the Express Insect Vector Set to achieveprotein expression in insect cells has the following advantages:

 Non-lytic, continuous protein expression

 Minimal degradation of your protein of interest as compared to viralexpression systems

 Higher protein expression level achieved than other similar non-viral insectexpression systems

 Choice of transient or stable protein expression

 Adapted for use with the Gateway Technology or traditional restrictionenzyme-mediated cloning

 

pXINSECT-DEST38载体特征:

pXINSECT-DEST38 and pXINSECT-DEST39 have the following features:

 The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)

 The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)

 A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)

 N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39

 Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone

 Chloramphenicol resistance gene located between the two attR sites for counterselection

 The ccdB gene located between the two attR sites for negative selection

 The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA

 Ampicillin resistance gene for selection of transformants in E. coli

 The pUC origin for high copy replication and maintenance of the plasmid in E. coli

 

pXINSECT-DEST38载体序列:

ATGTATAATTAATTAAATTCAAATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGT

CGGACAACGGAACCAGACCCTGTAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCT

CTCTCTTCGGTTCACTTTACTGATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGA

CTAGCGTCTACATCTTTAATCTCGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACC

GCTCTTCGTTTTTGAAAGACAATACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATA

CTGTTCGACAATGTGCATGCTGCCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTC

GCAATATTGTCATGATATTTATAATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCG

TACTTTTTTTGTTAACAATTAAATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTT

TACACTTTCCGAATACTTTAATATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCA

ATTTCATTACTCTCTTTACGACTAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGT

ACAACTTGCTCAAAAGAAACCCACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTG

CGCGTAATACATATCTAAATTAAAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAG

TGGCATTTTTTACAATTTTGTGCAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGC

CGGTTTCTTTAACCAAATTGTACGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATT

ATCTACCATGTAATAGTTGTTTGTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCA

AACGGCTTTACTTCACTTTTTTTAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACC

TATTGGAATTGTAGTCGTTGTCGTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGT

AATTTCTTGAGTTGAAGCAACCGTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAG

CTTTGAATTGTGCTTTTCTTGTATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTG

AATCGTCCAAGTATTCGTCCAGCTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATG

CTCAAAAGATTCCGCATTATAAGCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCC

GCATTATAAGCAGAACTAACTGCTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGT

TATCATCAGTTAACGAATTGACGCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTAC

CGTGTCGGCTCCATCCGGCGTGGGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAA

AACTCTGAATAGCCGTTGTCGAACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAAT

TAATTTGCGTCATAGTCGTTTGGTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAAT

ATTCAACATGAACGTCAATTTATACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCC

TCTAAAACCAATGCGGTTATCGTTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTA

ATGCAAAAACTCGTTTGGTGAGCGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAAC

ACCGTCCTTGAACGGAACACGAGGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGAT

TTATTTACATACAACAAGTACACTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAG

TGTGGCACATTTTGTCTGTGCGCAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGC

GCACACGGACAACGGACCCGACAAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTG

CATTTTATTAATAACTTACAAAATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCC

CGAACTGCAAATCGATCGATAAGCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAAT

TAATTTCTCCGCGTCGTATTATACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATG

ATTTTGATGATGGAGGTGCGCTCAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCA

ATTTGCTACGACGCAATTATTGTGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAA

AGTATATTGATATTAAATCATATAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATT

AAAATTGTTCAACGATTGTATGAGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATT

CTACTTGTAAAACACAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTT

AAAACTAAACTCGCTTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTT

TTCAAAACTGAACCGGCTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATT

AAACTGATGTCATTTTTATACACGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAAC

TCGCTTTACAAGTAGAATTCTACTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTG

TTTTCGTCATAAAATGTTTATACAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATT

CTACGTTACATTTAAATTCGTTGTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAAC

GTGCAACCAACGATTGTATTTGACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGC

ACGGCTTCTTTTTTTACCACTATCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACAT

AAGCGTACGCGTTTAAAACGTGCGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTT

GCGTAACTCGTTTAAAGCATTAATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGA

ACCGAGTAGTGGCATGAATAAAATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCG

CGGGTAGGTACCACCACCCTGCCTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGC

GGCCGTGGTACTGAGACCTTAGAACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATG

GGCTCCAGTAACCACTTAACATCAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAA

AATAAATATGTTTGAAGTCCGTAACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCT

TCACCGACAATGGAAAATAATCACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAG

TTTCGATAAAGCGAGGCTACCAAGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACA

ACTTTTACCTCTCAAATTGCCCACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAG

CCGCTACGATATCATTATCATACAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATC

TCTTCATAATTATAATTAAAAAGAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGA

GATTAATTAACCGGATTTTCTGGGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGA

AATTCTATGAAACTTCGGCGGGAGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCA

GTTATCAAGGCATCGCCTTATCAAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAA

ACTGAGTCAGCCCGCGATTGGTGGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCT

TTTATATAGTTTATCCTCACGAGTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATC

TACGTACTTGTCACTTATTTAATAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACA

TATTTATTTATTTGTAAAATTTGAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTT

TGAGTGTTTTTTTTTTTTAATAATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGT

GAAGTGAAATTTTTTATAAAAAAAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTAC

TAAATAAGAAATAAGTTTATTGGACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGA

AATTGGGAACAGTGAATTATGAATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAA

ATTTGTCGTTTACTTAGTATAATGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCA

GACTAATTCAAGAGGTGCGACGAAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCG

AATTCCTGCAGCCCAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAA

TGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAA

CATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA

TGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATC

ACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTG

CTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAA

GCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATG

GCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAA

CTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCA

AGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTC

TCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCC

CCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCA

TCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGG

CAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTG

ATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAG

CAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTC

CGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAAT

CAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACT

GGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGA

GTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAA

AGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATG

GCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAA

ACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCG

ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAA

TATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATCGACCCGGGT

CTAGAGCGGCCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAACGAAAGA

TTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACGAAACCA

CATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATTGTCCGG

TGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCATCGACA

ATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCCTCGCCT

CCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCATTGTACA

CAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGGTGGCTC

AAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATATGCATA

TTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTAAAGTTA

CTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACTTGCTGT

CCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGACAGTTA

CGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGAATGCGA

GTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTACTTTCT

ACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTAGAGGTA

CCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACACACAAG

ATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCATTGCGT

AGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTTAGTGAG

GGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAAT

TCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA

TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCG

GCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG

CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCA

GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT

TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG

GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT

CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT

CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT

TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCG

CCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA

AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC

CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT

TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG

ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTA

GATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT

TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC

TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCG

AGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGT

GGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGC

CAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT

GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG

GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGG

CAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAAC

CAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC

GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA

TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTAC

TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACA

CGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCA

TGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA

AGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCT

CATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTT

GAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAA

ACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCC

GTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGT

GGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTG

CGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGC

TGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG

TGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT

GAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATT

GCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACGTTGAAGTACCTTACACAAC

AAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCCAAAATCTAAAGCAAGGAGA

AGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGCAATTTTGCAAAACTTTACT

GTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAATCTTCGCAGAGTCAATAAAT

TGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAAGGCACTCCCGCCGGTTTCA

CAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATGGGCCGGGCTATCAATTATC

TAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAACGATGTCCCCAACATACGC

GGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTGCGGCGCATGGACCAACGTG

CCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAAAACTTCCCGGAGACCAACG

TGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTTACATAATCACATGAGAACC

TTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTTGTTTACCGCCGCCACATTA

GTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTGCAAGGTAGAAACGCCGGAG

AAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCAATCTAGCTCAGTCGGATGT

TAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAACCAACATGTGCCAAGGATTT

AACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAACCCAACTTCGCCTCAATACG

TAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTACACGTTTAGTGATTTAATT

GGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAATCGTCCAACGGTAGTGACA

GCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTGTTTTTAGGTTTAATACTTT

ATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTGGGGCACCAACTCCCATTGT

TGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAAGACAAAAATAATATAAAAT

ATATGTATAATTAATTAAATTCAAAAGAT

 

pXINSECT-DEST38其他昆虫表达载体:

pMT/BioEase-DEST

pVL1393

pXINSECT-DEST38

pBacPAK8

pIEX/Bac-1

pFastBacHT A

pMT/V5-His-TOPO

pCoHygro

pAc5.1-V5-His A

pAc5-V5-HisB

pCoBlast

pIZ/V5-His

pIZT/V5-His

pMIB/V5-His A

pMT/BiP/V5-His B

pIEx/Bac-3

pIEXBac-c-EGFP-1

pAc5.1B-EGFP

pBlueBacHis2 C

pFastBacHT C

pFBDM

pFastBac Dual

pMT/V5-His C

pMT/V5-His A

pAc5.1b

pVL1392-XyIE

pFastBac-N-GST-TEV

pFastBacHT-CAT

pVL1392

pXINSECT-DEST39

pBacPAK9

pIB/V5-His-TOPO

pFastBacHT B

pFastBac1

pMT/BiP/V5-His A

pAc5.1/V5-His C

pAc5/V5-HisA

pAc5-V5-HisC

pAc5.1/V5-His B

pIB/V5-His

pMIB/V5-His C

pMT/BiP/V5-His C

pIEx/Bac-4

pIEXBac-c-EGFP-3

pIEXBac-c-EGFP-4

pBlueBacHis2 A

pBlueBacHis2 B

pMT-Bip-V5-HisA

pUCDM

pMIB/V5-His B

pMT/V5-His B

pAcGP67-A

pAc5.1a

pFastBac-c-His-TEV

pFastBacI-Gus

pBADZ-His6Cre