pFastBacHT A
pFastBacHT A
编号 | 载体名称 |
北京华越洋生物 KCVECT130 | pFastBacHT A |
pFastBacHTA载体基本信息
载体名称: | pFastBacHTA, pFastBac HTA, pFastBacHT A, pFastBac HT A |
质粒类型: | 昆虫细胞表达载体；杆状病毒表达载体 |
启动子: | Polyhedrin |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 4856 bp |
5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
载体标签: | N-His tag |
载体抗性: | Ampicillin 氨苄 |
筛选标记: | Gentamicin 庆大霉素 |
备注: | 含TEV切割位点 |
稳定性: | 稳表达 Stable |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 杆状病毒 |
pFastBacHTA载体质粒图谱和多克隆位点信息
pFastBacHTA载体简介
Bac-to-BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后，在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具
有以下特点：
Þ 用于表达蛋白质的多角体强启动子
Þ 用于简化克隆的三个读码框
Þ 用于轻松纯化重组融合蛋白的 N-端 6xHis 标签
Þ 用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点
引用及参考文献
DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F,
J Biol Chem (2012) 287:31073-31084
Product usage: na
Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A;
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M;
J Biol Chem (2002) 277:9780-9789
Product usage:
Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu, and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.
pFastBacHTA载体序列
ORIGIN
1 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
61 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
121 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
181 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
241 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
301 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
361 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
421 AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT
481 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
541 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
601 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
661 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
721 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
781 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
841 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
901 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
961 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
1021 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
1081 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
1141 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
1201 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
1261 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
1321 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
1381 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
1441 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT
1501 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT
1561 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
1621 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
1681 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
1741 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
1801 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
1861 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
1921 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
1981 TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG
2041 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
2101 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
2161 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
2221 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG
2281 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC
2341 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG
2401 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCAGACCAGC CGCGTAACCT
2461 GGCAAAATCG GTTACGGTTG AGTAATAAAT GGATGCCCTG CGTAAGCGGG TGTGGGCGGA
2521 CAATAAAGTC TTAAACTGAA CAAAATAGAT CTAAACTATG ACAATAAAGT CTTAAACTAG
2581 ACAGAATAGT TGTAAACTGA AATCAGTCCA GTTATGCTGT GAAAAAGCAT ACTGGACTTT
2641 TGTTATGGCT AAAGCAAACT CTTCATTTTC TGAAGTGCAA ATTGCCCGTC GTATTAAAGA
2701 GGGGCGTGGC CAAGGGCATG GTAAAGACTA TATTCGCGGC GTTGTGACAA TTTACCGAAC
2761 AACTCCGCGG CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG
2821 TCGATATCAA AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG
2881 GGATCGTCAC CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA
2941 TGCTTGAGGA GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT
3001 GCGAGATCAT AGATATAGAT CTCACTACGC GGCTGCTCAA ACCTGGGCAG AACGTAAGCC
3061 GCGAGAGCGC CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA
3121 CGGAGCAAGT TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT
3181 CCGAACTCAC GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG
3241 AGCCTACATG TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG
3301 CCCTGCTGCG TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA
3361 TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA
3421 ACAGTCATAA CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA
3481 GGTTCTGGAC CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA
3541 GGCTTATGTC AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC
3601 CTTGGGCAGC AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC
3661 GGTCTCCACG CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG
3721 CACGGATCTG CCCTGGCTTC AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT
3781 GGTGCTGACC CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT
3841 GTTCGCCCAG GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CGTATACTCC GGAATATTAA
3901 TAGATCATGG AGATAATTAA AATGATAACC ATCTCGCAAA TAAATAAGTA TTTTACTGTT
3961 TTCGTAACAG TTTTGTAATA AAAAAACCTA TAAATATTCC GGATTATTCA TACCGTCCCA
4021 CCATCGGGCG CGGATCTCGG TCCGAAACCA TGTCGTACTA CCATCACCAT CACCATCACG
4081 ATTACGATAT CCCAACGACC GAAAACCTGT ATTTTCAGGG CGCCATGGAT CCGGAATTCA
4141 AAGGCCTACG TCGACGAGCT CAACTAGTGC GGCCGCTTTC GAATCTAGAG CCTGCAGTCT
4201 CGAGGCATGC GGTACCAAGC TTGTCGAGAA GTACTAGAGG ATCATAATCA GCCATACCAC
4261 ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA
4321 TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA
4381 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
4441 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC TGATCACTGC TTGAGCCTAG
4501 GAGATCCGAA CCAGATAAGT GAAATCTAGT TCCAAACTAT TTTGTCATTT TTAATTTTCG
4561 TATTAGCTTA CGACGCTACA CCCAGTTCCC ATCTATTTTG TCACTCTTCC CTAAATAATC
4621 CTTAAAAACT CCATTTCCAC CCCTCCCAGT TCCCAACTAT TTTGTCCGCC CACAGCGGGG
4681 CATTTTTCTT CCTGTTATGT TTTTAATCAA ACATCCTGCC AACTCCATGT GACAAACCGT
4741 CATCTTCGGC TACTTTTTCT CTGTCACAGA ATGAAAATTT TTCTGTCATC TCTTCGTTAT
4801 TAATGTTTGT AATTGACTGA ATATCAACGC TTATTTGCAG CCTGAATGGC GAATGG
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pFastBacHT A其他昆虫表达载体：
