pIEx/Bac-3

pIEx/Bac-3

pIEx-Bac-3载体基本信息

pIEx-Bac-3载体质粒图谱和多克隆位点信息

pIEx-Bac-3载体简介

The pIEx/Bac-3 vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

pIEx-Bac-3载体序列

ORIGIN     

          1 GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG

         61 AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC

        121 AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT

        181 GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG

        241 GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC

        301 CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT

        361 AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC

        421 TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT

        481 TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA

        541 TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC

        601 AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT

        661 CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT

        721 ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT

        781 TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT

        841 TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT

        901 TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA

        961 TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT

       1021 TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT

       1081 TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA

       1141 TCAATATATA GTTGCTGATC AGATCTGATC ATGGAGATAA TTAAAATGAT AAGGGCGCGT

       1201 AAAACACAAT CAAGTATGAG TCATAAGCTG ATGTCATGTT TTGCACACGG CTCATAACCG

       1261 AACTGGCTTT ACGAGTAGAA TTCTACTTGT AACGCACGAT CAGTGGATGA TGTCATTTGT

       1321 TTTTCAAATC GAGATGATGT CATGTTTTGC ACACGGCTCA TAAACTCGCT TTACGAGTAG

       1381 AATTCTACGT GTAACGCACG ATCGATTGAT GAGTCATTTG TTTTGCAATA TGATATCATA

       1441 CAATATGACT CATTTGTTTT TCAAAACCGA ACTTGATTTA CGGGTAGAAT TCTACTTGTA

       1501 AAGCACAATC AAAAAGATGA TGTCATTTGT TTTTCAAAAC TGAACTCGCT TTACGAGTAG

       1561 AATTCTACGT GTAAAACACA ATCAAGAAAT GATGTCATTT GTTATAAAAA TAAAAGCTGA

       1621 TGTCATGTTT TGCACATGGC TCATAACTAA ACTCGCTTTA CGGGTAGAAT TCTACGCGCG

       1681 TCGATGTCTT TGTGATGCGC GCGACATTTT TGTAGGTTAT TGATAAAATG AACGGATACG

       1741 TTGCCCGACA TTATCATTAA ATCCTTGGCG TAGAATTTGT CGGGTCCATT GTCCGTGTGC

       1801 GCTAGCATGC CCGTAACGGA CCTCGTACTT TTGGCTTCAA AGGTTTTGCG CACAGACAAA

       1861 ATGTGCCACA CTTGCAGCTC TGCATGTGTG CGCGTTACCA CAAATCCCAA CGGCGCAGTG

       1921 TACTTGTTGT ATGCAAATAA ATCTCGATAA AGGCGCGGCG CGCGAATGCA GCTGATCACG

       1981 TACGTCCCTC GTGTTCCGTT CAAGGACGGT GTTATCGACC TCAGATTAAT GTTTATCGGC

       2041 CGACTGTTTT CGTATCCGCT CACCAAACGC GTTTTTGCAT TAACATTGTA TGTCGGCGGA

       2101 TGTTCTATAT CTAATTTGAA TAAATAAACG ATAACCGCGT TGGTTTTAGA GGGCATAATA

       2161 AAAGAAATAT TGTTATCGTG TTCGCCATTA GGGCAGTATA AATTGACGTT CATGTTGGAT

       2221 ATTGTTTCAG TTGCAAGTTG ACACTGGCGG CGACAAGATC GTGAACAACC AAGTGACTCC

       2281 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC

       2341 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCCATGGCAC

       2401 ATCACCACCA TCATCACCAT CACCACCACG GTGCACTTGA AGTCCTCTTT CAGGGACCCG

       2461 GGTACCAGGA TCCTGTACAA GTCGACGCGG CCGCAGAGCT CGCTCTGGTG CCACGCGGTA

       2521 GTTCCGCTTG GAGCCACCCG CAGTTCGAAA AGTAAGTGAT TAACCTCAGG TTATACATAT

       2581 ATTTTGAATT TAATTAATTA TACATATATT TTATATTATT TTTGTCTTTT ATTATCGAGG

       2641 GGCCGTTGTT GGTGTGGGGT TTTGCATAGA AATAACAATG GGAGTTGGCG ACGTTGCTGC

       2701 GCCAACACCA CCTCCCTTCC CTCCTTTCAT CATGTATCTG TAGATAAAAT AAAATATTAA

       2761 ACCTAAAAAC AAGACCGCGC CTATCAACAA AATGATAGGC ATTAACTTGC CGCTGACGCT

       2821 GTCACTAACG TTGGACGATT TGCCGACTAA ACCTTCATCG CCCAGTAACC AATCTAGGTA

       2881 GCTGAGCGCA TGCAAGCTGA TCCGGGTTAT TAGTACATTT ATTAAGCGCT AGATTCTGTG

       2941 CGTTGTTGAT TTACAGACAA TTGTTGTACG TATTTTAATA ATTCATTAAA TTTATAATCT

       3001 TTAGGGTGGT ATGTTAGAGC GAAAATCAAA TGATTTTCAG CGTCTTTATA TCTGAATTTA

       3061 AATATTAAAT CCTCAATAGA TTTGTAAAAT AGGTTTCGAT TAGTTTCAAA CAAGGGTTGT

       3121 TTTTCCGAAC CGATGGCTGG ACTATCTAAT GGATTTTCGC TCAACGCCAC AAAACTTGCC

       3181 AAATCTTGTA GCAGCAATCT AGCTTTGTCG ATATTCGTTT GTGTTTTGTT TTGTAATAAA

       3241 GGTTCGACGT CGTTCAAAAT ATTATGCGCT TTTGTATTTC TTTCATCACT GTCGTTAGTG

       3301 TACAATTGAC TCGACGTAAA CACGTTAAAT AGAGCTTGGA CATATTTAAC ATCGGGCGTG

       3361 TTAGCTTTAT TAGGCCGATT ATCGTCGTCG TCCCAACCCT CGTCGTTAGA AGTTGCTTCC

       3421 GAAGACGATT TTGCCATAGC CACACGACGC CTATTAATTG TGTCGGCTAA CACGTCCGCG

       3481 ATCAAATTTG TAGTTGAGCT TTTTGGAATT ATTTCTGATT GCGGGCGTTT TTGGGCGGGT

       3541 TTCAATCTAA CTGTGCCCGA TTTTAATTCA GACAACACGT TAGAAAGCGA TGGTGCAGGC

       3601 GGTGGTAACA TTTCAGACGG CAAATCTACT AATGGCGGCG GTGGTGGAGC TGATGATAAA

       3661 TCTACCATCG GTGGAGGCGC AGGCGGGGCT GGCGGCGGAG GCGGAGGCGG AGGTGGTGGC

       3721 GGTGATGCAG ACGGCGGTTT AGGCTCAAAT GTCTCTTTAG GCAACACAGT CGGCACCTCA

       3781 ACTATTGTAC TGGTTTCGGG CGCCGTTTTT GGTTTGACCG GTCTGAGACG AGTGCGATTT

       3841 TTTTCGTTTC TAATAGCTTC CAACAATTGT TGTCTGTCGT CTAAAGGTGC AGCGGGTTGA

       3901 GGTTCCGTCG GCATTGGTGG AGCGGGCGGC AATTCAGACA TCGATGGTGG TGGTGGTGGT

       3961 GGAGGCGCTG GAATGTTAGG CACGGGAGAA GGTGGTGGCG GCGGTGCCGC CGGTATAATT

       4021 TGTTCTGGTT TAGTTTGTTC GCGCACGATT GTGGGCACCG GCGCAGGCGC CGCTGGCTGC

       4081 ACAACGGAAG GTCGTCTGCT TCGAGGCAGC GCTTGGGGTG GTGGCAATTC AATATTATAA

       4141 TTGGAATACA AATCGTAAAA ATCTGCTATA AGCATTGTAA TTTCGCTATC GTTTACCGTG

       4201 CCGATATTTA ACAACCGCTC AATGTAAGCA ATTGTATTGT AAAGAGATTG TCTCAAGCTC

       4261 GGATCGATCC CGCACGCCGA TAACAAGCCT TTTCATTTTT ACTACAGCAT TGTAGTGGCG

       4321 AGACACTTCG CTGTCGTCGA GGTTTAAACG CTTCCTCGCT CACTGACTCG CTGCGCTCGG

       4381 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG

       4441 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC

       4501 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA

       4561 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

       4621 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC

       4681 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC

       4741 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC

       4801 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT

       4861 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

       4921 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA

       4981 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA

       5041 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA

       5101 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG

       5161 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

       5221 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG

       5281 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT

       5341 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG

       5401 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA

       5461 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

       5521 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC

       5581 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT

       5641 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA

       5701 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT

       5761 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

       5821 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA

       5881 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG

       5941 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA

       6001 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA

       6061 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

       6121 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC

       6181 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG

       6241 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA

       6301 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC

       6361 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

       6421 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA

       6481 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC

       6541 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA

       6601 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT

       6661 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

       6721 CGTTTACAAT TTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC

       6781 GGGCCTCTTC GCTATTACGC CA

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pIEx-Bac-3其他昆虫表达载体：