pXINSECT-DEST39
pXINSECT-DEST39
编号 | 载体名称 |
北京华越洋生物 KCVECT142 | pXINSECT-DEST39 |
pXINSECT-DEST39载体基本信息
载体名称: | pXINSECT-DEST39 |
质粒类型: | 昆虫表达载体；Gateway载体 |
克隆方法: | Gateway |
启动子: | Actin |
载体大小: | 12447 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | 6XHis tag （N-末端） |
载体抗性: | 氨苄青霉素、氯霉素（仅空载体） |
真核筛选标记: | 无 |
克隆菌株: | DB3.1 |
宿主细胞（系）: | High Five（推荐） 等细胞系 |
备注: | pXINSECT-DEST39载体与pBmA-neo载体共转染，可以建立稳表达细胞系；应用Gateway技术，可快速高效将 目的基因片段（尤其适用于目的基因片段>1kb，用传统的连接酶法效率低的）插入到载体中； |
瞬表达/稳表达: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pXINSECT-DEST39载体质粒图谱和多克隆位点信息
pXINSCET-DEST39载体简介
pXINSCET-DEST39载体优势：
Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
l Non-lytic, continuous protein expression
l Minimal degradation of your protein of interest as compared to viral expression systems
l Higher protein expression level achieved than other similar non-viral insect expression systems
l Choice of transient or stable protein expression
l Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning
pXINSCET-DEST39载体特征：
l The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
l The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
l A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
l N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
l Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
l Chloramphenicol resistance gene located between the two attR sites for counterselection
l The ccdB gene located between the two attR sites for negative selection
l The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
l Ampicillin resistance gene for selection of transformants in E. coli
l The pUC origin for high copy replication and maintenance of the plasmid in E. coli
pXINSCET-DEST39载体序列
GAGGTCGACGGTATCGATTGCAGGTCGATATTTAAAAAAAATTATAATAATGTTAAAGTTGCTTCATACG
TTGAAGTACCTTACACAACAAATAATGCAGACGTCGAAATGACTGACATAACAAATGCGCCCTTTCGCCC
AAAATCTAAAGCAAGGAGAAGATTAGATTTTACCAACATGGCGCCGCAGCCGTGTTGTATTGACGACGGC
AATTTTGCAAAACTTTACTGTGATATATTTATTAAATTAAGTTTGCTTTAAAATGAGTTTTTTTACAAAT
CTTCGCAGAGTCAATAAATTGTATCCTAATCAGGCCAGTTTTCTTGCTGATAATACGCGTCTTTTAACAA
GGCACTCCCGCCGGTTTCACAAATGTGCTCAACGCGCCCAGTGTACGCAACCTTGGAAACAACAGATATG
GGCCGGGCTATCAATTATCTAACAACCGGTTTGTGAGCACTTCAGACATAAACAGAATTACTCGTAACAA
CGATGTCCCCAACATACGCGGAGTATTTCAGGGCATTTCAGACCCTCAAATAAACTCATTGAGCCAATTG
CGGCGCATGGACCAACGTGCCAGACTTTCATTACCACACCAAACAGACGCGATCCAATGCAGTCAGACAA
AACTTCCCGGAGACCAACGTGCGCACGCCCGAAGGTGTTCAAAATGCACTGCAGCAAAACCCCCCCGTTT
ACATAATCACATGAGAACCTTGAAAGTAGCAGGAGTGGGCATACTCTTGGGCCGGCGGCGGTTATCTTTT
GTTTACCGCCGCCACATTAGTACAAGATATAATCAACGCCATCAATAGAACCGGCGGAAGTTATTATGTG
CAAGGTAGAAACGCCGGAGAAAACGCCGAGGCCTGTTTGTTATTGCAGCGCACTTGTCGTCAAGACCGCA
ATCTAGCTCAGTCGGATGTTAACATTTGCTCAAGAGACCCCTTGTTGGCTAACGATTCGCCCCTACTAAC
CAACATGTGCCAAGGATTTAACTATGAAACAGAAAAAACAGTTTGTCGCGGCAGCAATCCGGCCGCTAAC
CCAACTTCGCCTCAATACGTAGATATTAGCGATCTTCTGCGGGCCAAACAATCATGTGCATCGAACCTTA
CACGTTTAGTGATTTAATTGGCGACTTGCGTTTACATTGGTTACTGGGAAGAGAAGGTTTAATCGGCAAA
TCGTCCAACGGTAGTGACAGCATCCGCAACAAAATAATGCCTCATCATTATGATGATAGGCGCGTTCTTG
TTTTTAGGTTTAATACTTTATTTTATCTACAGATACATGACAAAAGGAGGAGGAGGAGGAGGAAGCGGTG
GGGCACCAACTCCCATTGTTGTTATTATGCAACACCCCACATCAACAGCGGCCCCTCGTCGATAATAAAA
GACAAAAATAATATAAAATATATGTATAATTAATTAAATTCAAAAGATATGTATAATTAATTAAATTCAA
ATTTTTTATATTTACAATTTAGTTTTTGTTCCGCAAACGTTATAGCGTCGGACAACGGAACCAGACCCTG
TAATATTAAAGCTAACAATTTTAACAAATTATTGTGCAATGTAGTGCTCTCTCTTCGGTTCACTTTACTG
ATTACAAACATGTGATGCTTAAATCTATTATATTTTTGAATTACTTGACTAGCGTCTACATCTTTAATCT
CGCCAGAAATCCAATAAAACTCTTCGTTTTTCTTAGCTATAGTCAACCGCTCTTCGTTTTTGAAAGACAA
TACTATAAAATTGTGACCTTTTACATTATCCACATTCTGAGTCAAATACTGTTCGACAATGTGCATGCTG
CCGTCCTCCTTCTTAACCTTTTTTAAATTTTCAGCGTTATTATTACTCGCAATATTGTCATGATATTTAT
AATTATTAAACAAAAGATTAGCGACACTACTGTATTTGTACGTGAGCGTACTTTTTTTGTTAACAATTAA
ATTTAAATTGTCCACCACATATTTGTTTGGGGGATTGTCGGGAAACTTTACACTTTCCGAATACTTTAAT
ATTTGACTCACATACGGCGATACAAAAAAATTATTAGATGCAGTCTCAATTTCATTACTCTCTTTACGAC
TAAGCATAATAGGCAAAGTAAATAAATTTTTATCTTGATACATTTCGTACAACTTGCTCAAAAGAAACCC
ACACTTTCTTTCGCCCAACGATTGTAACAAAGTCACAAATGTGGTTTGCGCGTAATACATATCTAAATTA
AAATATGAAGTCAGAGCAGCTTTAAACGTGTGATGCACATCGACAAAGTGGCATTTTTTACAATTTTGTG
CAGCCGTCTCGTCGTTGCACACATCTTGAGAATGAGGAATTTCTATGCCGGTTTCTTTAACCAAATTGTA
CGAGATCATAAATCTAATTTTATCAAAAGTTACCACAAACACGCGATTATCTACCATGTAATAGTTGTTT
GTATATTCGTACACCACATTGCTCACGTACTTGGCAAATATAATTTCAAACGGCTTTACTTCACTTTTTT
TAACCACAAACATGTAATAACCAGTTTCGGACATATGGTCGGAGAACCTATTGGAATTGTAGTCGTTGTC
GTCGAAACGCATCAAATACGGCGCAAAATCATTAGTAAAATAATGCGTAATTTCTTGAGTTGAAGCAACC
GTGCAAATGTTCGTGTTGTGATTAATTGTCTGCTCAAGGGTTGCACAGCTTTGAATTGTGCTTTTCTTGT
ATTTAGGCTTCAATTTATTCTTGTTAAATTGGCCCACCACACTTTGTGAATCGTCCAAGTATTCGTCCAG
CTTCCGTTTAGTTCCAGTTGCCGATGGTTGGTTCACACCAACAGGATGCTCAAAAGATTCCGCATTATAA
GCAGAACTGGGCGATGGTTGCTCCGCAACAGGCAGCTCAAAAGATTCCGCATTATAAGCAGAACTAACTG
CTTCTCCGAGATTATCAGTGGTCTTGAGCAAACATTCCATTATATCGTTATCATCAGTTAACGAATTGAC
GCTTGCCAAAAAGTTTGAAGCTGCCTGCAGTCTGCTGTCAGATACTACCGTGTCGGCTCCATCCGGCGTG
GGATTGTTATAATAATTCAAATAGTCGTTGGGCTGTTGTTTATCACAAAACTCTGAATAGCCGTTGTCGA
ACGACGCTCGGGACGGCGTCGGAGCACTGGTGTACGACGCGTTAAAATTAATTTGCGTCATAGTCGTTTG
GTTGTTCACGATCGTGTCCCGCCAATGTCAACTTGCAACTGAAACAATATTCAACATGAACGTCAATTTA
TACTGCCCTAATGGCGAACACGATAATAATATTTTTTTTATTATGCCCTCTAAAACCAATGCGGTTATCG
TTTATTTATTCAAATTAGATACAGAACATCCGCCGACATACAATGTTAATGCAAAAACTCGTTTGGTGAG
CGGATACGAAAACAGTCGGCCGATAAACATTAATCTGAGGTCGATAACACCGTCCTTGAACGGAACACGA
GGAGCGTACGTGATCAGCTGCATTCGCGCGCCGCGCCTTTATCGAGATTTATTTACATACAACAAGTACA
CTGCGCCGTTGGCATTTGTGGTAACGCGCACACAAGCAGAGCTGCAAGTGTGGCACATTTTGTCTGTGCG
CAAAACCTTTGAAGCCAAAAGCACAAGGTCCGTTACGGGCATGCTAGCGCACACGGACAACGGACCCGAC
AAATTCTACGCCAAGGATTTAATGATAATGTCGGGCAACGTGTCGGTGCATTTTATTAATAACTTACAAA
ATGTCGCGCGCATCACAAAGACATTGATATATTTAAACATTTATGTCCCGAACTGCAAATCGATCGATAA
GCTTGATATCGAATTCCTGCAGCCCAATATTACGTTCGTGCCAGAAATTAATTTCTCCGCGTCGTATTAT
ACGATTTATACGGTACAGCAGCTTGGCCCACAAATAGATCGTTTTATGATTTTGATGATGGAGGTGCGCT
CAAGATGAAACCCATTCAGACGTTATTAGTTGCGTCAAGTATTTGGCAATTTGCTACGACGCAATTATTG
TGGAAGAAGCGTAATTTGTGAACAGCCCATTCGAGGCTAGATTGAAAAAGTATATTGATATTAAATCATA
TAAATTGTTTATGAGGCCTTCAAACGAATCTTGTAAAGATTATTTATTAAAATTGTTCAACGATTGTATG
AGAGGGTCATTTGTTTTTCAAAACTGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAG
AGATGATGTCATTTGTTTTTCAAAACTGAATGATGTCATTTGTTTTTTAAAACTAAACTCGCTTTTACGA
GTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAACCGGCTTTAC
GAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATCATTAAACTGATGTCATTTTTATACA
CGATTGTTAACATGTTTAATAATGACTAATTTGTTTTTCAAATTAAACTCGCTTTACAAGTAGAATTCTA
CTTGTAACGCACGATTAAAATTATTATAATCAGGAATGATGTCATTTGTTTTCGTCATAAAATGTTTATA
CAACGGAATCTTCTTGTAAATTATCCAAATAATATAATTTATCCGATTCTACGTTACATTTAAATTCGTT
GTTATCGTACAATTCTTCAGGACACGCCATGTATTGGCCGTTTTTAACGTGCAACCAACGATTGTATTTG
ACGCCGTCGTTGGATTGCGTGTTCAGGTTGGCGTACACGTGACTGGGCACGGCTTCTTTTTTTACCACTA
TCGCATCTTCGTCGTACGCGGATCTACAACCAATCCCGTTGCCCACATAAGCGTACGCGTTTAAAACGTG
CGATAGGTCTTTGGCCAATTCGCAATCAGCGTCCACTTTAACGTTGTTGCGTAACTCGTTTAAAGCATTA
ATAATGACGTCATTTTCCGCATGACAACTGGTTAGCTTGAAAAACGGAACCGAGTAGTGGCATGAATAAA
ATAAATCTTTGTTGTCTAATATTGGGGGGGAGCTCTTGTGAGTCCTCGCGGGTAGGTACCACCACCCTGC
CTATTTCTGCCGTGAAGCAGTAATGCGTTTCGGTTTGAAGAGTGGGGCGGCCGTGGTACTGAGACCTTAG
AACTCATATCTGAAGGTGGGTGGCACATTTACGTTGTAGATGTCTATGGGCTCCAGTAACCACTTAACAT
CAGGTGGGCTGTGAGCTCTTACACCCATCTACGCAATAAAAAATTAAAAATAAATATGTTTGAAGTCCGT
AACATAGATTCCGTATTTTTACAGTTGTTTTTCACGTTTTTCATTTCTTCACCGACAATGGAAAATAATC
ACACACAAATACACTGTATAGTAACAACGAGCAGAGCCGATTTTGGAGTTTCGATAAAGCGAGGCTACCA
AGAATGCGGCAGATAAGATTTACGTACATTCAAGAGTCGCTGATAACAACTTTTACCTCTCAAATTGCCC
ACAGTGCGATCACAAGAAACATAGACGAACGGATCTGTGCGCAACGAGCCGCTACGATATCATTATCATA
CAGATTTTTATCTTTTCATCTAGCTTCAGTTAGTGATGCTTTCTGATCTCTTCATAATTATAATTAAAAA
GAATAAATTATCTAGTAATATAGTTCTACTACGGTACACGAATTTTGAGATTAATTAACCGGATTTTCTG
GGTTATGATTTACATCGGTACAGAATCTAGTGAAAGCACGTCGAGTGAAATTCTATGAAACTTCGGCGGG
AGTCGGGGAGAGGTTACAAGCGACCGCGAGGTGCCGCTAACTTAATCAGTTATCAAGGCATCGCCTTATC
AAAAGATGCGAGCTGATAGCGTGCGCGTTACCATATATGGTGACAAAAACTGAGTCAGCCCGCGATTGGT
GGAAAAACAAACTGGAGCCGATACTGTGTAAATTGTGATAACGGCTCTTTTATATAGTTTATCCTCACGA
GTCGGTTCTCATTTACTAAGGTGTGCTCGAACAGTGCGCATTCGCATCTACGTACTTGTCACTTATTTAA
TAATACTATGTAAGTTTTAATTTTAAAATTGCGAAAGAAAAAAAAACATATTTATTTATTTGTAAAATTT
GAATTTCGAAGGTTCTCCGTCCCTTTACCTTTAAGTATTACATATGTTTGAGTGTTTTTTTTTTTTAATA
ATACGCTAATGATAACGTGTTACGTTACATAATTGTTGCATAACTAGTGAAGTGAAATTTTTTATAAAAA
AAAACATTTTTCGGAATTTAGTGTACTGCAGATGTTAATAAACACTACTAAATAAGAAATAAGTTTATTG
GACGCACATTTCAAAGTGTCCACTCGCATCGATCAATTCGGAAACAGAAATTGGGAACAGTGAATTATGA
ATCTTATACAGTTTTCTTTAACGTCACTAAATAGATGGACGCAAATAAATTTGTCGTTTACTTAGTATAA
TGTATGGAATGAGAATGTAGTTTGAATTGTTTTTTTTCTTTTCTTGCAGACTAATTCAAGAGGTGCGACG
AAGAAGTTGCCGCGTTGGTAGTAGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCAGCATATG
CGCGGTTCGCATCACCATCACCATCACAGCGCTGGATCCTCGATCACAAGTTTGTACAAAAAAGCTGAAC
GAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATA
CTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTT
CCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCA
TTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCG
TAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC
GGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTT
TTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTAC
ACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAA
TATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGAC
AACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG
CGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTA
CTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCG
TATTTGCGCGCACCGGTGCTAGCGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTAT
TACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGG
TAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGG
GATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAAT
GCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATT
ATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCC
GTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGT
GCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATT
AACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGT
GACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGA
TATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAATTAATTAAGATCGAAT
CTAGAGCGGCCGCCACCGCCTCGAGAAGTCTTACGAACTTCCCGACGGTCAGGTCATCACCATCGGAAAC
GAAAGATTCCGTTGCCCAGAGGCCCTCTTCCAACCCTCGTTCTTGGGTATGGAAGCCAACGGAATCCACG
AAACCACATACAACTCCATCATGAAGTGCGACGTGGACATCCGTAAGGACTTGTACGCCAACACCGTATT
GTCCGGTGGTACCACCATGTACCCTGGAATCGCCGACCGTATGCAAAAGGAAATCACACGTCTCGCCCCA
TCGACAATGAAGATTAAGATCATCGCTCCCCCAGAGAGGAAGTACTCCGTATGGATCGGTGGATCGATCC
TCGCCTCCCTCTCTACCTTCCAACAGATGTGGATCTCGAAACAGGAGTACGACGAGTCTGGTCCCTCCAT
TGTACACAGGAAGTGCTTCTAAGCGTTGAGACTTTAAGTTATGATGCCCTACAGCAGAACCTCAAGAGGG
TGGCTCAAATTACGCTTGTGATCTTGTAAATAAATTCAGTATTTAATGTAGGTTGTAAGGTATTGTAATA
TGCATATTACGTAAAACGAACGGAATGTTGTTGTTGCCGTTTTTTTTTTGACAAAGATTTTTATTTATTA
AAGTTACTAACCCCAAAACTTTTTAATAAAATAAATTTATATACCGGTATAATAACTGACGTTTTTCACT
TGCTGTCCCCGCTCCCGACTAACAGTACGTCGTGTGCACCGAAATTACCGATTTCGTACACCGTTTGAGA
CAGTTACGCTAGGAGCACAAATCTCCCAGCTGCATACCGTTGTTTACTGCAGCTGCAGTCTTTAATTGGA
ATGCGAGTCGTTGACCGCTTAATACGAAACATTCTAAAATTCGCAAAATGCAAAGGAAACTGGTTCTGTA
CTTTCTACCTTTCAAAAGATTCACCAAATTAATTTTATGCGGACTCACTAATTCCGTAGAAATCTGTGTA
GAGGTACCCAGGTTACGCTTAGGCATAAGATGACTGTTCGCGTTTTTACAATACATACGAGCAGGTTACA
CACAAGATGAACATCCTTTGATGCGTCTGTGTCTTGACCCGTCTGAGATTTGAGTGACTTGTCAACGTCA
TTGCGTAGTGTCACCGGTCGTCGAGATCCCCGCCGCGGTGGAGCTACGAGCTCCAGCTTTTGTTCCCTTT
AGTGAGGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT
CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAA
CTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAAT
GAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTC
GCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACA
GAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT
CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACC
CCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGAC
TTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT
TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTT
CACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCT
GACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG
CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT
ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGC
AGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA
GTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT
TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA
AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG
TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTA
CTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGAT
AATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCT
CAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC
TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG
GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATT
GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCC
CCGAAAAGTGCCACCTGGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAA
TCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGAT
AGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGG
CGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGA
GGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGC
GAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTC
ACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCAT
TCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG
GCCAGTGAATTGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTC
pXINSECT-DEST39其他昆虫表达载体：
