pGL4.75

pGL4.75载体基本信息

pGL4.75载体质粒图谱和多克隆位点信息

pGL4.75载体简介

The pGL4.75[hRluc/CMV] Vector(a-c) encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.

The pGL4.75[hRluc/CMV] Vector contains the hRluc reporter gene and a CMV immediate-early enhancer/promoter and can be used as an expression control or a co-reporter vector.

pGL4.75载体序列

ORIGIN

    1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT ATAGCATAAA

   61 TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA TCATAATATG TACATTTATA

  121 TTGGCTCATG TCCAATATGA CCGCCATGTT GGCATTGATT ATTGACTAGT TATTAATAGT

  181 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA

  241 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA

  301 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT

  361 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA

  421 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG

  481 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT

  541 TTTGGCAGTA CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC

  601 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT

  661 GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT

  721 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCACTAG AAGCTTTATT GCGGTAGTTT

  781 ATCACAGTTA AATTGCTAAC GCAGTCAGTG GGCCTCGGCG GCCAAGCTTG GCAATCCGGT

  841 ACTGTTGGTA AAGCCACCAT GGCTTCCAAG GTGTACGACC CCGAGCAACG CAAACGCATG

  901 ATCACTGGGC CTCAGTGGTG GGCTCGCTGC AAGCAAATGA ACGTGCTGGA CTCCTTCATC

  961 AACTACTATG ATTCCGAGAA GCACGCCGAG AACGCCGTGA TTTTTCTGCA TGGTAACGCT

 1021 GCCTCCAGCT ACCTGTGGAG GCACGTCGTG CCTCACATCG AGCCCGTGGC TAGATGCATC

 1081 ATCCCTGATC TGATCGGAAT GGGTAAGTCC GGCAAGAGCG GGAATGGCTC ATATCGCCTC

 1141 CTGGATCACT ACAAGTACCT CACCGCTTGG TTCGAGCTGC TGAACCTTCC AAAGAAAATC

 1201 ATCTTTGTGG GCCACGACTG GGGGGCTTGT CTGGCCTTTC ACTACTCCTA CGAGCACCAA

 1261 GACAAGATCA AGGCCATCGT CCATGCTGAG AGTGTCGTGG ACGTGATCGA GTCCTGGGAC

 1321 GAGTGGCCTG ACATCGAGGA GGATATCGCC CTGATCAAGA GCGAAGAGGG CGAGAAAATG

 1381 GTGCTTGAGA ATAACTTCTT CGTCGAGACC ATGCTCCCAA GCAAGATCAT GCGGAAACTG

 1441 GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG AGAAGGGCGA GGTTAGACGG

 1501 CCTACCCTCT CCTGGCCTCG CGAGATCCCT CTCGTTAAGG GAGGCAAGCC CGACGTCGTC

 1561 CAGATTGTCC GCAACTACAA CGCCTACCTT CGGGCCAGCG ACGATCTGCC TAAGATGTTC

 1621 ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC GCTATTGTCG AGGGAGCTAA GAAGTTCCCT

 1681 AACACCGAGT TCGTGAAGGT GAAGGGCCTC CACTTCAGCC AGGAGGACGC TCCAGATGAA

 1741 ATGGGTAAGT ACATCAAGAG CTTCGTGGAG CGCGTGCTGA AGAACGAGCA GTAATTCTAG

 1801 AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA

 1861 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT

 1921 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT

 1981 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA

 2041 TGTGGTAAAA TCGATAAGGA TCCGTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG

 2101 CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT

 2161 CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC

 2221 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT

 2281 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG

 2341 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG

 2401 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT

 2461 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA

 2521 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT

 2581 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC

 2641 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA

 2701 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG

 2761 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG

 2821 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT

 2881 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA

 2941 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC

 3001 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA

 3061 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA

 3121 CTTGGTCTGA CAGCGGCCGC AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG

 3181 TGAGGCACCG ATCTCAGCGA TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT

 3241 CGTGTAGATC ACTACGATTC GTGAGGGCTT ACCATCAGGC CCCAGCGCAG CAATGATGCC

 3301 GCGAGAGCCG CGTTCACCGG CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC

 3361 CGAGCGAAGA AGTGGTCCTG CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG

 3421 TGATGCTAGA GTAAGAAGTT CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC

 3481 TGGCATCGTG GTATCACGCT CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG

 3541 GTCAAGCCGG GTCACATGAT CACCCATATT ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC

 3601 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT

 3661 ACACAATTCT CTTACCGTCA TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC

 3721 AACCAAGTCG TTTTGTGAGT AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT

 3781 ACGGGACAAC ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC

 3841 TTCGGGGCGG AAAGACTCAA GGATCTTGCC GCTATTGAGA TCCAGTTCGA TATAGCCCAC

 3901 TCTTGCACCC AGTTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA

 3961 AACAGGCAAG CAAAATGCCG CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT

 4021 CATACTCGTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA

 4081 AGGATAAGTA AGTAATATTA AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT

 4141 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGTACTA ACATACGCTC

 4201 TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG

 4261 CAGGTGCCAG AACATTTCTC T

//

其他哺乳动物表达载体：