北京华越洋生物
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首页    载体及质粒    pGL4.20
1

pGL4.20

pGL4.20

pGL4.20

 

编号

载体名称

北京华越洋生物VECT6144

pGL4.20

 

pGL4.20载体基本信息

载体名称:

pGL4.20 pGL4.20[luc2/Puro]

质粒类型:

哺乳细胞表达

高拷贝/低拷贝:

--

启动子:

无启动子

克隆方法:

多克隆位点,限制性内切酶

载体大小:

5404bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

嘌呤霉素(puromycin

备注:

pGL4.20载体含Luciferase荧光素酶报告基因,无启动子,用于研究目的启动子的功能。

稳定性:

--

组成型:

组成型

病毒/非病毒:

非病毒

 

pGL4.20载体质粒图谱和多克隆位点信息


 



 

pGL4.20载体序列

ORIGIN

    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC

   61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT

  121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC

  181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC

  241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG

  301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG

  361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC

  421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC

  481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA

  541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC

  601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC

  661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC

  721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC

  781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC

  841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC

  901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG

  961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC

 1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG

 1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC

 1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC

 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC

 1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT

 1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC

 1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC

 1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC

 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG

 1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA

 1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC

 1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG

 1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA

 1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT

 1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC

 1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA

 1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG

 2041 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT

 2101 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA

 2161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA

 2221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA

 2281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA

 2341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG

 2401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT

 2461 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GACCGAGTAC AAGCCTACCG

 2521 TGCGCCTGGC CACTCGCGAT GATGTGCCCC GCGCCGTCCG CACTCTGGCC GCCGCTTTCG

 2581 CCGACTACCC CGCTACCCGG CACACCGTGG ACCCCGACCG GCACATCGAG CGTGTGACAG

 2641 AGTTGCAGGA GCTGTTCCTG ACCCGCGTCG GGCTGGACAT CGGCAAGGTG TGGGTAGCCG

 2701 ACGACGGCGC GGCCGTGGCC GTGTGGACTA CCCCCGAGAG CGTTGAGGCC GGCGCCGTGT

 2761 TCGCCGAGAT CGGCCCCCGA ATGGCCGAGC TGAGCGGCAG CCGCCTGGCC GCCCAGCAGC

 2821 AAATGGAGGG CCTGCTTGCC CCCCATCGTC CCAAGGAGCC TGCCTGGTTT CTGGCCACTG

 2881 TAGGAGTGAG CCCCGACCAC CAGGGCAAGG GCTTGGGCAG CGCCGTCGTG TTGCCCGGCG

 2941 TAGAGGCCGC CGAACGCGCC GGTGTGCCCG CCTTTCTCGA AACAAGCGCA CCAAGAAACC

 3001 TTCCATTCTA CGAGCGCCTG GGCTTCACCG TGACCGCCGA TGTCGAGGTG CCCGAGGGAC

 3061 CTAGGACCTG GTGTATGACA CGAAAACCTG GCGCCTAATG ATCTAGAACC GGTCATGGCC

 3121 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA

 3181 GATGCTGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC

 3241 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC

 3301 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG

 3361 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG

 3421 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG

 3481 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA

 3541 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT

 3601 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC

 3661 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG

 3721 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC

 3781 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA

 3841 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG

 3901 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT

 3961 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC

 4021 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA

 4081 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA

 4141 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT

 4201 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCGGC

 4261 CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT CAGTGAGGCA CCGATCTCAG

 4321 CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC CGTCGTGTAG ATCACTACGA

 4381 TTCGTGAGGG CTTACCATCA GGCCCCAGCG CAGCAATGAT GCCGCGAGAG CCGCGTTCAC

 4441 CGGCCCCCGA TTTGTCAGCA ATGAACCAGC CAGCAGGGAG GGCCGAGCGA AGAAGTGGTC

 4501 CTGCTACTTT GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG TCGTGATGCT AGAGTAAGAA

 4561 GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC TACTGGCATC GTGGTATCAC

 4621 GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA GCGGTCAAGC CGGGTCACAT

 4681 GATCACCCAT ATTATGAAGA AATGCAGTCA GCTCCTTAGG GCCTCCGATC GTTGTCAGAA

 4741 GTAAGTTGGC CGCGGTGTTG TCGCTCATGG TAATGGCAGC ACTACACAAT TCTCTTACCG

 4801 TCATGCCATC CGTAAGATGC TTTTCCGTGA CCGGCGAGTA CTCAACCAAG TCGTTTTGTG

 4861 AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC TATACGGGAC AACACCGCGC

 4921 CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG TTCTTCGGGG CGGAAAGACT

 4981 CAAGGATCTT GCCGCTATTG AGATCCAGTT CGATATAGCC CACTCTTGCA CCCAGTTGAT

 5041 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CGGGGTGTGC AAAAACAGGC AAGCAAAATG

 5101 CCGCAAAGAA GGGAATGAGT GCGACACGAA AATGTTGGAT GCTCATACTC GTCCTTTTTC

 5161 AATATTATTG AAGCATTTAT CAGGGTTACT AGTACGTCTC TCAAGGATAA GTAAGTAATA

 5221 TTAAGGTACG GGAGGTATTG GACAGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG

 5281 TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG CTCTCCATCA AAACAAAACG

 5341 AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT

 5401 CTCT

//

 

其他哺乳动物表达载体:

pEBVHis   A

pcDNA5/TO

pDsRed2-Bid

pNFκB-MetLuc2-Reporter

pGL4.10

pBApo-CMV-neo

pAcGFP1-N1

pEF1α-IRES-DsRed-Express2

pGL4.29

pDsRed-Monomer

pSecTag2   A

pCMV-DsRed-Express2

pGL4.13

pIRES

pGL4.27

pcDNA3.1/NT-GFP-TOPO

pG5   luciferase

pIRES-hrGFP-1a

pGL4.26

pEF1α-IRES-ZsGreen1

pCMV-AD

pDsRed-Express2-N1

pACT

pCMV-Tag   2A

pRevTet-Off

pCMV-Tag   3B

pBIND-Id   Control

pCMV-Tag   5B

pTet-Off

pCRE-hrGFP

pTRE2

pAcGFP1-C   In-Fusion Ready

pTRE2-hygro

pDsRED2-Mito

pRevTRE

p3XFLAG-CMV-14

pVgRxR

pAcGFP1-F

pTK-hyg

p3XFLAG-CMV-8

pOPI3CAT

pAcGFP1-C1

pTRE3G-Luc

pFLAG-CMV-2

pBK-RSV

pAsRed2-C1

pSwitch

pcDNA3.3-TOPO

pIRES2-DsRed2

pAsRed2-N1

pcDNA4/His   C

pcDNA6.2/cLumio-DEST

pCMV-Myc

pAcGFP1-Lam

c-Flag   pcDNA3

pCMV-tdTomato

pCMV-Tag   2C

pAcGFP1-C

pcDNA4/TO/Myc-His   A

pAcGFP1-Mito

pCMV-Tag   5A

pSEAP2-Basic

pcDNA6/myc-His   B

pAcGFP1-N   In-Fusion Ready

pCMV-Tag   3C

pBI-CMV3

pcDNA6/V5-His   B

pDsRed-Monomer-N   In-Fusion Ready

p3XFLAG-CMV-7

pNFkB-DD-tdTomato

pcDNA6.2/nTC-Tag-DEST

pcDNA4/TO/Myc-His   B

p3XFLAG-CMV-9

pcDNA3.1/His   A

pOptiVEC-TOPO

pIRES2-EGFP

pFLAG-CMV-4

pEBVHis   B

pcDNA5/FRT

pcDNA3.1/His   C

pBI-CMV4

pGL4.75

pGL4.30

pcDNA3.1/CT-GFP-TOPO

pcDNA4/His   A

pGL4.20

pGL4.19

pEF1α-IRES-AcGFP1

pcDNA4/myc-His   B

pCMV-SPORT6

pACT-MyoD

pcDNA3.2/V5/GW/D-TOPO

pcDNA4/HisMax   C

pCMV-SPORT6

pCMV-BD

pcDNA4/TO/Myc-His/LacZ

pCMV-Tag   4A

pBIND

pCMV-Tet3G

pcDNA4/HisMax-TOPO

pcDNA6/myc-His   C

pBD-NF-κB

pTet   on advanced

p3XFLAG-CMV-13

pCMV-Tag   2B

pRevTet-On

pTRE-Tight

p3xFLAG-CMV-10

pGRN145

pTet-On

pIND

pFLAG-CMV-3

pCMV-MEK1

pTRE3G

pGene/V5-His   B

pcDNA4/TO/Myc-His   C

pCMV-Tag   3A

pcDNA4/TO

pOPRSVI

pcDNA6.2/C-YFP-DEST

pCMVLacI

pcDNA4/His   B

pcDNA4/HisMax   A

pcDNA6.2/cTC-Tag-DEST

pBI-CMV1

pcDNA4/HisMax   B

pIRESpuro3

pcDNA6.2/nGeneBLAzer-DEST

pEF1α-AcGFP1-N1

pcDNA4/myc-His   C

pIRESneo3

pCRE-MetLuc2-Reporter

pCMV-LacZ

pcDNA3.1/His   B

pIRESneo2

pEF1α-DsRed-Express2

pCMV-Tag   4B

pcDNA6/V5-His   C

pcDNA4/myc-His   A

pDsRed-Express-C1

pCMV-Tag   5C

pCHO1.0

pCMV-PKA

pEF1α-DsRed-Monomer-N1

plRES2-ZsGreen1

pGL3-Promoter

pAcGFP1-N3

pDD-AmCyan1   Reporter

p3XFLAG-CMV-7.1

pCMV-MEKK1

pcDNA5/FRT/TO

pCRE-DD-AmCyan1

pFLAG-CMV-5a

pFLAG-CMV2

pBApo-CMV-Pur

pIRES2-DsRed-Express2

pBudCE4.1

pAcGFP1-C2

pBApo-EF1α-pur

pDsRed-Express-N1

pREP4

ptdTomato-C1

ptdTomato-N1

pcDNA6.2/nLumio-DEST

pBApo-CMV

pCRE-DD-tdTomato

pAcGFP1-Golgi

pcDNA6/myc-His   A

pIRES2-AcGFP1

pAcGFP1-Hyg-C1

pAcGFP1-p53

pcDNA6/V5-His   A

pIREShyg3

pAcGFP1-Mem

pAcGFP1-Actin

pcDNA5/FRT/TO-TOPO

pcDNA6/TR

pAcGFP1-C3

pBI-CMV2

pcDNA6.2/V5/GW/D-TOPO

pDsRed-Express2-C1

pTT5

pEF1α-tdTomato

pcDNA6.2/cGeneBLAzer-DEST

pBI-CMV5

pSEAP2-Control

pEF1α-tdTomato

pcDNA6.2/nGeneBLAzer-GW/D-TOPO

pAmCyan1-C1

pSEAP2-Control